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Citation
Petesch, S.J., Lis, J.T. (2008). Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci.  Cell 134(1): 74--84.
FlyBase ID
FBrf0205070
Publication Type
Research paper
Abstract

To efficiently transcribe genes, RNA Polymerase II (Pol II) must overcome barriers imposed by nucleosomes and higher-order chromatin structure. Many genes, including Drosophila melanogaster Hsp70, undergo changes in chromatin structure upon activation. To characterize these changes, we mapped the nucleosome landscape of Hsp70 after an instantaneous heat shock at high spatial and temporal resolution. Surprisingly, we find an initial disruption of nucleosomes across the entire gene within 30 s after activation, faster than the rate of Pol II transcription, followed by a second further disruption within 2 min. This initial change occurs independently of Pol II transcription. Furthermore, the rapid loss of nucleosomes extends beyond Hsp70 and halts at the scs and scs' insulating elements. An RNAi screen of 28 transcription and chromatin-related factors reveals that depletion of heat shock factor, GAGA Factor, or Poly(ADP)-Ribose Polymerase or its activity abolishes the loss of nucleosomes upon Hsp70 activation.

PubMed ID
PubMed Central ID
PMC2527511 (PMC) (EuropePMC)
Related Publication(s)
Note

Clearing the way for unpaused polymerases.
Weake and Workman, 2008, Cell 134(1): 16--18 [FBrf0205544]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference