Reference Report

Reference
Citation	Arya, G.H., Lodico, M.J.P., Ahmad, O.I., Amin, R., Tomkiel, J.E. (2006). Molecular characterization of teflon, a gene required for meiotic autosome segregation in male Drosophila melanogaster. Genetics 174(1): 125--134. (Export to RIS)
FlyBase ID	FBrf0194210
Publication Type	Research paper
PubMed ID	16816414
PubMed Abstract	Drosophila melanogaster males lack recombination and have evolved a mechanism of meiotic chromosome segregation that is independent of both the chiasmatic and achiasmatic segregation systems of females. The teflon (tef) gene is specifically required in males for proper segregation of autosomes and provides a genetic tool for understanding recombination-independent mechanisms of pairing and segregation as well as differences in sex chromosome vs. autosome segregation. Here we report on the cloning of the tef gene and the molecular characterization of tef mutations. Rescue experiments using a GAL4-driven pUAS transgene demonstrate that tef corresponds to predicted Berkeley Drosophila Genome Project (BDGP) gene CG8961 and that tef expression is required in the male germ line prior to spermatocyte stage S4. Consistent with this early prophase requirement, expression of tef was found to be independent of regulators of meiotic M phase initiation or progression. The predicted Tef protein contains three C2H2 zinc-finger motifs, one at the amino terminus and two in tandem at the carboxyl terminus. In addition to the zinc-finger motifs, a 44- to 45-bp repeat is conserved in three related Drosophila species. On the basis of these findings, we propose a role for Tef as a bridging molecule that holds autosome bivalents together via heterochromatic connections.
DOI	10.1534/genetics.106.061556
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(2R)P803-Δ15
Alleles (25)
	aly^unspecified bol^unspecified can^unspecified fat-spondin^k15914 mia^unspecified Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} pelo^unspecified sa^unspecified Scer\GAL4^c135 Scer\GAL4^c729 Scer\GAL4^T76 Scer\GAL4^T98 Scer\GAL4^T100 Scer\GAL4^T110 Scer\GAL4^T155 T:Avic\GFP^EGFP tef^k15914 tef^{Scer\UAS.T:Avic\GFP-EGFP} tef^Z1869 tef^Z3455 tef^Z4169 tef^Z5549 tef^Z5864 twe^unspecified w^+mC
Constructs (2)
	P{UAS-mCD8::GFP.L} P{UAS-tef.EGFP}
Genes (20)
	aly bol can Dpse\fat-spondin Dpse\GA21233 Dpse\GA21437 Dsim\fat-spondin Dsim\tef Dyak\fat-spondin Dyak\tef fat-spondin mia Mmus\Cd8a pelo rgr sa Scer\GAL4 tef twe w
Insertions (8)
	P{GawB}c135 P{GawB}c729 P{GawB}l(2)T76^T76 P{GawB}T98 P{GawB}T100 P{GawB}T110 P{GawB}T155 P{lacW}tef^k15914
Natural transposons (1)
	P-element