FlyBase Allele Report: Dmel\arm[8]

arm⁸ somatic stem cell (SSC) clones in the ovary are lost slightly more rapidly than wild-type clones, but are lost at a slower rate than arm² clones. arm⁸ follicle cell clones have normal morphology compared to neighbouring wild-type follicle cells.

(Song and Xie, 2003)

arm² has no dramatic effect on germline stem cell (GSC) maintenance in the ovary; approximately 60% of mutant GSCs observed in ovaries during the first week after clone induction are maintained for two more weeks (compared to 70% for controls).

(Song et al., 2002)

Mutant embryos have segment polarity defects, although head involution and dorsal closure are normal.

(Grevengoed et al., 2001)

arm⁸ embryos show a similar tracheal branching phenotype to arm⁴ embryos.

(Chihara and Hayashi, 2000)

Homozygous clones in the wing show no disturbance in planar polarity.

(Axelrod et al., 1998)

Homozygous clones in eyes show normal ommatidial polarity.

(Boutros et al., 1998)

arm⁸ embryos derived from homozygous female germ line clones (lacking both zygotic and maternal arm function) lack RP2 neurons. They show a broad array of central nervous system defects, including gaps in the longitudinals and poorly formed commissures.

(Loureiro and Peifer, 1998)

Embryos incubated at the restrictive temperature after acridine orange injection show increased cell death in a similar pattern to that seen for wg^l-12 embryos shifted at stage 11. Cell death in the rest of the segment is similar to wild type.

(Pazdera et al., 1998)

Homozygous clones in the eye are extremely small and only infrequently observed.

(Wehrli and Tomlinson, 1998)

Temperature-sensitive allele. Homozygous clones are recovered at reasonable frequency in the wing pouch of discs shifted to the restrictive temperature 18 hours before the end of the third larval instar stage, but not in discs shifted to the restrictive temperature 48 hours before the end of the third larval instar stage. Homozygous clones are produced in the part of the disc that gives rise to the dorsal thorax under both conditions.

(Neumann and Cohen, 1997)

Embryos that are zygotically and maternally mutant for arm⁸ have a very strong segment polarity phenotype, and secrete only denticles with no naked cuticle. Embryos that are zygotically and maternally mutant for arm⁸ and are expressing arm^{S10.Scer\UAS.T:Hsap\MYC} (using Scer\GAL4^e22c) essentially secrete only naked cuticle.

(Pai et al., 1997)

Embryos exhibit severe segment polarity defects.

(Orsulic and Peifer, 1996)

Dorsal tracheal trunks exhibit irregular width and the connections between lateral trunks is incomplete.

(Uemura et al., 1996)

When arm⁸; arm^BCD7/+ flies are shifted to 25^oC during the third larval instar the resulting adults have missing pattern elements along the wing margin and alula. The abdomens lack sternites.

(Couso et al., 1994)

Embryos lacking both maternal and zygotic sgg and arm function have an embryonic cuticle phenotype like that of arm mutant embryos alone. Embryos lacking maternal sgg and arm but receiving wild type paternal copies develop into normal embryos that survive until adulthood. Embryos lacking maternal sgg and arm but receiving a wild type paternal copy of arm⁺ only, show a sgg naked cuticle phenotype.

(Peifer et al., 1994)

Clonal analysis reveals that oogenesis is normal, including with respect to cytoplasmic actin distribution, but reduction in maternal contribution enhances the embryonic phenotype. Eggs derived from mutant germlines are laid, and have normal anterior-posterior and dorso-ventral patterning.

(Peifer et al., 1993)

Very strong segment polarity phenotype. Homozygous clones survive at a higher frequency in the dorsal regions of the leg disc, this biased distribution is produced by differential survival, not directed migration.

(Peifer et al., 1991)

Late third instar larvae have very small imaginal discs, pupae die without differentiating any adult cuticle.

(Peifer and Wieschaus, 1990)

temperature-sensitive allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

arm⁸ has lethal | embryonic stage | recessive phenotype, non-enhanceable by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Jones et al., 2010)

Suppressed by

Statement

Reference

arm⁸ has lethal | recessive | embryonic stage phenotype, suppressible by tum[+]/tum^AR2

(Jones et al., 2010)

arm⁸ has lethal | recessive | embryonic stage phenotype, suppressible by pav[+]/pav^B200

(Jones et al., 2010)

NOT suppressed by

Statement

Reference

arm⁸ has lethal | recessive | embryonic stage phenotype, non-suppressible by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Jones et al., 2010)

Enhancer of

Statement

Reference

arm⁸/arm[+] is an enhancer of partially lethal phenotype of lqfR⁰³⁶⁸⁵/lqfR^Δ117

(Lee and Fischer, 2012)

NOT Enhancer of

Statement

Reference

arm⁸/arm[+] is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Suppressor of

Statement

Reference

arm⁸/arm[+] is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Apc2^g10/Apc2[+], brat^DG19310/brat¹¹

(Komori et al., 2014)

arm⁸ is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^wor.PA, klu^UAS.Tag:HA

(Komori et al., 2014)

NOT Suppressor of

Statement

Reference

arm⁸/arm[+] is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Other

Statement

Reference

arm⁸/arm[+], lqfR⁰³⁶⁸⁵/lqfR^Δ117 has lethal - all die during P-stage phenotype

(Lee and Fischer, 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

arm⁸ has abdominal ventral denticle belt | embryonic stage phenotype, non-enhanceable by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Jones et al., 2010)

arm⁸ has phenotype, non-enhanceable by Apc2^ΔS

(McCartney et al., 1999)

Suppressed by

Statement

Reference

arm⁸ has abdominal ventral denticle belt | embryonic stage phenotype, suppressible by tum[+]/tum^AR2

(Jones et al., 2010)

arm⁸ has abdominal ventral denticle belt | embryonic stage phenotype, suppressible by pav[+]/pav^B200

(Jones et al., 2010)

NOT suppressed by

Statement

Reference

arm⁸ has abdominal ventral denticle belt | embryonic stage phenotype, non-suppressible by Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Jones et al., 2010)

arm⁸ has phenotype, non-suppressible by Apc2^ΔS

(McCartney et al., 1999)

Enhancer of

Statement

Reference

arm⁸ is an enhancer of eye phenotype of Src42A^KR.hs.2sev

(Takahashi et al., 2005)

NOT Enhancer of

Statement

Reference

arm⁸/arm[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Suppressor of

Statement

Reference

arm⁸ is a suppressor | partially of type II neuroblast | increased number phenotype of Scer\GAL4^wor.PA, klu^UAS.Tag:HA

(Komori et al., 2014)

arm⁸/arm[+] is a suppressor | partially of type II neuroblast | increased number phenotype of Apc2^g10/Apc2[+], brat^DG19310/brat¹¹

(Komori et al., 2014)

arm⁸ is a suppressor of ventral denticle belt | germline clone | rescuable maternal effect phenotype of sgg^M11

(Tolwinski et al., 2003)

arm⁸ is a suppressor of embryonic/first instar larval cuticle | germline clone | rescuable maternal effect phenotype of sgg^M11

(Tolwinski et al., 2003)

NOT Suppressor of

Statement

Reference

arm⁸/arm[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

arm⁸ is a non-suppressor of phenotype of Apc^Q8

(Ahmed et al., 1998)

arm⁸ is a non-suppressor of phenotype of dsh^hs.sev.B

(Boutros et al., 1998)

Other

Statement

Reference

arm⁸/arm[+], lqfR⁰³⁶⁸⁵/lqfR^Δ117 has wing | P-stage phenotype

(Lee and Fischer, 2012)

arm⁸/arm[+], lqfR⁰³⁶⁸⁵/lqfR^Δ117 has adult head capsule | P-stage phenotype

(Lee and Fischer, 2012)

arm⁸/arm[+], lqfR⁰³⁶⁸⁵/lqfR^Δ117 has eye | P-stage phenotype

(Lee and Fischer, 2012)

arm⁸/arm[+], lqfR⁰³⁶⁸⁵/lqfR^Δ117 has leg | P-stage phenotype

(Lee and Fischer, 2012)

Src42A^KR.hs.2sev, arm⁸ has ommatidium phenotype

(Takahashi et al., 2005)

Src42A^KR.hs.2sev, arm⁸ has interommatidial bristle phenotype

(Takahashi et al., 2005)

arm⁸, ena^GC1 has embryo | dorsal closure stage phenotype

(Grevengoed et al., 2001)

arm⁸, ena^GC1 has embryo | embryonic stage 14 phenotype

(Grevengoed et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of supernumerary neuroblasts which is seen in clones derived from single type II neuroblasts expressing klu^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wor.PA is partially suppressed if the clones are also mutant for arm⁸.

(Komori et al., 2014)

arm⁸/+ ; lqfR⁰³⁶⁸⁵/lqfR^Δ117 animals die in the pupal case. They have small or absent eyes, small head capsules and also have morphological defects in the head cuticle, wings and legs.

(Lee and Fischer, 2012)

Heterozygosity for either tum^AR2 or pav^B200 restores areas of naked cuticle between the belts to almost wide-type levels, and many more arm⁸ mutants are able to hatch into the first instar stage.

Heterozygosity for a Rac1^J10 Rac2^Δ Mtl^Δ triple mutant chromosome does not alter arm⁸ mutant patterning nor its rate of hatching.

(Jones et al., 2010)

p130CAS¹ homozygotes in combination with heterozygous arm⁸ severely reduces viability.

(Tikhmyanova et al., 2010)

Expression of the Src42A^KR.hs.2sev transgene in a arm⁸ background results in a rough eye phenotype.

(Takahashi et al., 2005)

arm⁸/Y ; ena^GC1/ena^GC1 embryos have strong defects in dorsal closure and head involution, with no change in the segment polarity phenotype compared to arm⁸/Y embryos.

(Grevengoed et al., 2001)

The arm⁸ mutant phenotype is not affected if the embryos are also maternally and zygotically homozygous for Apc2^ΔS.

(McCartney et al., 1999)

Photoreceptors are not rescued from death in Apc^Q8 homozygotes carrying one copy of arm⁸.

(Ahmed et al., 1998)

Embryos derived from homozygous doubly mutant germ lines, receiving neither maternal or zygotic wild type product, for sgg^M11 and arm⁸ the ventral cuticle is covered with denticles.

(Siegfried et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8557

arm⁸ P{FRT(w^hs)}101/FM7a

68159

y¹ arm⁸ P{neoFRT}19A/FM7a

Notes on Origin

Discoverer

Comments

There is a phenotypic series in the clonal germline phenotype that parallels the affect in embryos.

(Peifer et al., 1993)

arm⁸; arm^BCD7/+ is a synthetic temperature sensitive allelic combination: flies are wild type at 17^oC and mutant at 25^oC.

(Couso et al., 1994)

Encodes a truncated protein that can still be phosphorylated.

(Peifer et al., 1994)

Association of arm protein with α-Cat and shg in vivo is assayed by SDS-PAGE and immunoblotting.

(Pai et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

arm⁸

(Greco et al., 2022, Arbeille and Bashaw, 2018, Komori et al., 2014, Lee and Fischer, 2012, Djiane and Mlodzik, 2010, Jones et al., 2010, Popodi et al., 2010-, Tikhmyanova et al., 2010, Chao et al., 2007, Corrigall et al., 2007)

arm^H8.6

(Chao et al., 2007, Takahashi et al., 2005, Bajpai et al., 2004, Kubota et al., 2003, Prasad et al., 2003, Tolwinski et al., 2003, Yang et al., 2002, Grevengoed et al., 2001, Tolwinski and Wieschaus, 2001, Chihara and Hayashi, 2000, Cox et al., 1999, Gallet et al., 1999, McCartney et al., 1999, Sato et al., 1999, Ahmed et al., 1998, Axelrod et al., 1998, Boutros et al., 1998, Loureiro and Peifer, 1998, Pazdera et al., 1998, Waltzer and Bienz, 1998, Wehrli and Tomlinson, 1998, Neumann and Cohen, 1997, Pai et al., 1997, Azpiazu et al., 1996, Cox et al., 1996, Miller and Moon, 1996, Orsulic and Peifer, 1996, Pai et al., 1996, Uemura et al., 1996, Peifer, 1995, Couso et al., 1994, Peifer et al., 1994, Peifer et al., 1994, Siegfried et al., 1994, Peifer et al., 1993, Peifer et al., 1993, van den Heuvel et al., 1993)

arm^H8.6

(Jones et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (53)

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