FlyBase Allele Report: Dmel\ph-p[409]

General Information

Symbol

Dmel\ph-p⁴⁰⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0013767

Feature type

allele

Associated gene

Dmel\ph-p

Associated Insertion(s)

Carried in Construct

Also Known As

ph⁴⁰⁹

Key Links

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Cheng et al., 1994)

loss of function allele

(O'Dor et al., 2006)

Mutagen

X ray

(Dura et al., 1987)

Progenitor genotype

Caused by aberration

In(1)ph⁴⁰⁹

Cytology

Polytene chromosomes normal? Chromosome mutant for ph and wapl but not for the intervening Pgd; probably carries an inversion with breaks in ph and wapl (Dura et al., 1987).

Description

Inversion breakpoint in ph-p.

(Hodgson et al., 1997)

Breakpoint within ph-p.

(de Camillis and Brock, 1994)

Mutation in ph-p.

(Santamaria, 1993.6)

Inversion breaks ph-p and wapl.

(Dura et al., 1987)

Deletion in the proximal repeat.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | embryonic stage

(O'Dor et al., 2006)

viable

(Hodgson et al., 1997, Fauvarque et al., 1995)

Phenotype Manifest In

anaphase | embryonic stage

(O'Dor et al., 2006)

nucleus | embryonic stage

(O'Dor et al., 2006)

sex comb

(Wang and Brock, 2003)

sex comb (with Df(3R)ry27)

(Wang and Brock, 2003)

sex comb (with Df(3R)ry619)

(Wang and Brock, 2003)

sex comb (with Tp(3;3)MRS)

(Wang and Brock, 2003)

telophase | embryonic stage

(O'Dor et al., 2006)

Detailed Description

Statement

Reference

ph-p⁴⁰⁹ embryos from homozygous or heterozygous mothers exhibit segregation defects at anaphase and telophase. Late anaphase nuclei are frequently connected by chromatin bridges; these bridges can be thick which make the joined nuclei an irregular size, or be thin and appear as threadlike chromatin connections between sets of sister chromatids. These chromatin bridges persist to telophase. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance.

ph-p⁴⁰⁹ embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage. 2% of these embryos show a "mitotic catastrophe" phenotype, characterized by completely disorganized chromosomes that have no regular pattern or normal ploidy and very few, abnormally large nuclei that may be amorphous or may have a normal, spherical shape.

(O'Dor et al., 2006)

ph-p⁴⁰⁹/+ heterozygous mutant flies exhibit a higher than wild-type average number of legs with sex combs, with an average of 2.7 legs with sex combs, compared to 2.0 in wild-type, although this is not statistically significant.

ph-p⁴⁰⁹/Df(3R)ry27 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 3.6 legs with sex combs, compared to 2.8 in ph-p⁴⁰⁹/+ heterozygotes.

ph-p⁴⁰⁹/Df(3R)ry619 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 5.1 legs with sex combs, compared to 4.1 in ph-p⁴⁰⁹/Tp(3;3)MRS heterozygotes.

(Wang and Brock, 2003)

Mutation changes the level of w expression in ph-p^lac+3 flies; eye colour is darker.

(Fauvarque et al., 1995)

Viable.

(Cheng et al., 1994)

Viable when hemizygous and homozygous.

(Dura et al., 1987)

Adults viable.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scm⁴, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm⁵, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^R5-13, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^D1, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^H1, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^M56, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^Suz302, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^D2, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^ET50, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^K2, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Scm^M36, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

ph-p⁴⁰⁹ has sex comb phenotype, enhanceable by Hsc70-4¹⁹⁵/Hsc70-4[+]

(Wang and Brock, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

ph-p⁴⁰⁹/Hsc70-4¹⁹⁵ mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 4.8 legs with sex combs, compared to 2.7 in ph-p⁴⁰⁹/+ heterozygotes.

(Wang and Brock, 2003)

ph-p⁴⁰⁹ completely suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Pc, and substantially suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Scm or esc.

(Roseman et al., 2001)

ph-p⁴⁰⁹ males that are also heterozygous for Scm^M56, Scm^D1, Scm^Suz302, Scm^M36, Scm⁴, Scm^D2, Scm^H1, Scm^ET50, Scm^R5-13, Scm⁵ or Scm^K2 show partial lethality.

(Bornemann et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ph-p⁴⁰⁹

ph⁴⁰⁹

(Doheny et al., 2008, O'Dor et al., 2006, Wang and Brock, 2003, Maurange and Paro, 2002, Hodgson et al., 2001, Roseman et al., 2001, Randsholt et al., 2000, Bornemann et al., 1998, Hodgson et al., 1997, Fauvarque et al., 1995, de Camillis and Brock, 1994)

Name Synonyms

Secondary FlyBase IDs

FBal0032414

References (14)

Report Sections

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