Open Close
General Information
Symbol
Hsap\TP53UAS.cYa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Yamaguchi
FlyBase ID
FBal0104764
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a wild-type Hsap\TP53 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Hsap\TP53Scer\UAS.cYa results in a dosage dependent eye degeneration phenotype.

Flies expressing one copy of Hsap\TP53Scer\UAS.cYa under the control of one copy of Scer\GAL4GMR.PS have severely abnormal eyes which are very rough in appearance. The ommatidia lack the regular hexagonal shape seen in wild-type flies and either additional or missing bristles are apparent. The phenotype is more severe if the flies are carrying two copies of Hsap\TP53Scer\UAS.cYa; many ommatidia appear fused, rhabdomeres with irregular shapes are seen and many ommatidia lack pigment cells. In eye discs expressing one copy of Hsap\TP53Scer\UAS.cYa under the control of one copy of Scer\GAL4GMR.PS incorporation of BrdU in the S phase zone corresponding to the second mitotic wave is reduced compared to wild type. In eye discs expressing two copies of Hsap\TP53Scer\UAS.cYa under the control of one copy of Scer\GAL4GMR.PS the S phase zone corresponding to the second mitotic wave is almost abolished. There is a significant increase in cell death posterior to the furrow in these eye discs. Extensive cell death is only seen in the very posterior of the disc.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Hsap\TP53UAS.cYa has eye phenotype, non-suppressible by TER94[+]/TER9426-8

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

BacA\p35GMR.PH is a strong suppressor of the rough eye phenotype caused by expression of Hsap\TP53Scer\UAS.cYa under the control of Scer\GAL4GMR.PS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\TP53Scer\UAS.cYa
Hsap\TP53UAS.cYa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Yamaguchi
Secondary FlyBase IDs
    References (2)