FlyBase Allele Report: Dmel\cic[fet-U6]

General Information

Symbol

Dmel\cic^fet-U6

Species

D. melanogaster

Name

FlyBase ID

FBal0130456

Feature type

allele

Associated gene

Dmel\cic

Associated Insertion(s)

Carried in Construct

Also Known As

cic^fetU6

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Goff et al., 2001)

Mutagen

ethyl methanesulfonate

(Goff et al., 2001)

Progenitor genotype

Cytology

Description

Amber mutation in exon 4.

(Goff et al., 2001)

Amino acid replacement: ??term.

(Goff et al., 2001)

Nucleotide substitution: C3007T.

(Goff et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:20,296,056..20,296,056 [+]

Nucleotide change:

C20296056T

Reported nucleotide change:

C?T

Amino acid change:

Comment:

Approximate location of amber mutation cic^fet-U6 in cic based on FBrf0141486.

(Goff et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage | somatic clone

(Jin et al., 2015)

increased occurrence of cell division | adult stage (with cic^fet-E11)

(Jin et al., 2015)

lethal | embryonic stage | germline clone | maternal effect | recessive

(Goff et al., 2001)

lethal | larval stage | recessive

(Goff et al., 2001)

lethal - all die during embryonic stage | maternal effect (with cic^fet-E11)

(Goff et al., 2001)

some die during embryonic stage | maternal effect (with cic^fet-E11)

(Goff et al., 2001)

some die during embryonic stage | maternal effect | recessive | somatic clone

(Goff et al., 2001)

viable (with cic^fet-E11)

(Goff et al., 2001)

visible (with cic^fet-E11)

(Goff et al., 2001)

Phenotype Manifest In

adult midgut (with cic^fet-E11)

(Jin et al., 2015)

adult midgut | somatic clone

(Jin et al., 2015)

dorsal appendage (with cic^BA53)

(Atkey et al., 2006)

dorsal appendage (with cic^fet-E11)

(Atkey et al., 2006, Goff et al., 2001)

dorsal appendage (with cic^fet-T6)

(Atkey et al., 2006)

egg (with cic^BA53)

(Atkey et al., 2006)

egg (with cic^fet-E11)

(Atkey et al., 2006)

egg (with cic^fet-T6)

(Atkey et al., 2006)

egg chorion (with cic^fet-E11)

(Goff et al., 2001)

egg chorion | maternal effect | somatic clone

(Lachance et al., 2009)

egg chorion | somatic clone

(Goff et al., 2001)

embryonic/first instar larval cuticle (with cic¹)

(Goff et al., 2001)

embryonic/first instar larval cuticle | somatic clone

(Goff et al., 2001)

embryonic epidermis (with cic^fet-E11)

(Goff et al., 2001)

intestinal stem cell | adult stage (with cic^fet-E11)

(Jin et al., 2015)

intestinal stem cell | somatic clone

(Jin et al., 2015)

wing (with cic^fet-E11)

(Goff et al., 2001)

wing vein (with cic^fet-E11)

(Goff et al., 2001)

Detailed Description

Statement

Reference

Homozygous cic^fet-U6 intestinal stem cell somatic clones (generated by MARCM) are larger than control clones and contain more cells.

Number of mitotic cells per midgut is significantly increased in cic^fet-U6/cic^fet-E11 transheterozygous adults.

(Jin et al., 2015)

Eggs derived from egg chambers containing complete homozygous follicle cell clones have a dorsalised eggshell.

(Lachance et al., 2009)

Eggs from mosaic homozygous cic^fet-U6 females exhibit ventral and lateral foci of ectopic appendage-like materail. Analysis of homozygous cic^fet-U6 follicle cell clones confirms that these foci are associated with ventral and lateral clones of cic^fet-U6 mutant clones. This ectopic appendage material is restricted to the anterior half of the eggshell, while posterior clones exhibit no detectable phenotype. Interestingly, ectopic appendage material at the extreme anterior of the eggshell often appears as appendage-like projections, suggesting that only cic^fet-U6 mutant follicle cells in the corresponding region of the columnar follicular epithelium can form an appendage-producing epithelial tube.

With the exception of those at the dorsal midline, cic^fet-U6 mutant follicle cell clones undergo apical constriction reminiscent of that observed in the endogenous appendage-producing populations. In addition, the follicle cell nuclei appear more closely packed in both the endogenous appendage roof primordia and in regions of anterior cic^fet-U6 mutant cells, presumably as a consequence of this apical constriction.

Approximately 12.9% of cic^fet-E11/cic^fet-U6 females exhibit a weak eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally with a trace of ventral collar material. The dorsalmost region remains free of appendage material.

Approximately 19.3% of cic^fet-U6/cic^fet-E11 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 30.0% of cic^fet-U6/cic^fet-E11 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 14.1% of cic^fet-U6/cic^BA53 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 85.9% of cic^fet-U6/cic^BA53 exhibit a severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.

Approximately 32.8% of cic^fet-U6/cic^fet-T6 females exhibit a weak-moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 20.9% of cic^fet-U6/cic^fet-T6 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 1.7% of cic^fet-U6/cic^fet-T6 exhibit a severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.

(Atkey et al., 2006)

Eggs derived from cic^fet-U6/cic^fet-E11 females are dorsalised. The eggs are shorter than normal and have broader dorsal appendages that are shifted laterally, with appendage base material encircling the entire anterior circumference of the egg. Embryos derived from cic^fet-U6/cic^fet-E11 females fail to hatch and are dorsalised (the secreted cuticle consists entirely of dorsal epidermis). cic^fet-U6/cic^fet-E11 adults have smaller wings than normal, with vein thickening and blistering. 22% of eggs produced by females with homozygous germline clones are dorsalised (78% are wild type), but 100% of the embryos derived from these eggs fail to hatch (no cellularisation is seen in these embryos). 34% of eggs derived from females carrying homozygous follicle cell clones are dorsalised (66% are wild type). 54% of the embryos derived from these females fail to hatch (of these, 10% are locally dorsalised along the anterior-posterior axis and 1% are completely dorsalised), and 46% hatch. Dorsal or lateral follicle cell clones do not result in defects in the dorso-ventral patterning of the embryo, while ventral follicle cell clones produce local dorsalisation in the embryo. cic¹/cic^fet-U6 females produce embryos with a tor gain-of-function phenotype.

(Goff et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

cic^fet-U6/cic^fet-T6 has dorsal appendage phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

cic^fet-U6/cic^fet-T6 has egg phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

cic^fet-U6/cic^fet-E11 has dorsal appendage phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

cic^fet-U6/cic^fet-E11 has egg phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

cic^fet-U6/cic^BA53 has dorsal appendage phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

cic^fet-U6/cic^BA53 has egg phenotype, suppressible by mirr^6D1

(Atkey et al., 2006)

NOT suppressed by

Statement

Reference

cic^fet-U6/cic^fet-T6 has dorsal appendage phenotype, non-suppressible by grk³/grk^2B6

(Atkey et al., 2006)

cic^fet-U6/cic^fet-T6 has egg phenotype, non-suppressible by grk³/grk^2B6

(Atkey et al., 2006)

cic^fet-U6/cic^fet-T6 has dorsal appendage phenotype, non-suppressible by rho⁶

(Atkey et al., 2006)

cic^fet-U6/cic^fet-T6 has egg phenotype, non-suppressible by rho⁶

(Atkey et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 0.5% of cic^fet-T6/mirr^6D1 cic^fet-U6 females exhibit a weak eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally with a trace of ventral collar material. Approximately 42.6% of cic^fet-U6/mirr^6D1 cic^fet-T6 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 42.1% of cic^fet-U6/mirr^6D1 cic^fet-T6 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. None of the cic^fet-U6/mirr^6D1 cic^fet-T6 females exhibit a severe eggshell phenotype (compared to 1.7% in the heterozygote). There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cic^fet-T6/cic^fet-U6 mutant, indicating that mirr^6D1 suppresses the cic^fet-T6/cic^fet-U6 phenotype.

Approximately 34.4% of cic^fet-E11/mirr^6D1 cic^fet-U6 females exhibit a weak eggshell phenotype. Approximately 37.4% of cic^fet-U6/mirr^6D1 cic^fet-E11 females exhibit a weak-moderate eggshell phenotype. Approximately 24.5% of cic^fet-U6/mirr^6D1 cic^fet-E11 females exhibit a moderate eggshell phenotype. Approximately 37.9% of cic^fet-U6/mirr^6D1 cic^fet-E11 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cic^fet-E11/cic^fet-U6 mutant, indicating that mirr^6D1 suppresses the cic^fet-E11/cic^fet-U6 phenotype.

Approximately 44.6% of cic^fet-U6/mirr^6D1 cic^fet-T6 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 37.9% of cic^fet-U6/mirr^6D1 cic^fet-E11 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 0.5% of cic^fet-U6/mirr^6D1 cic^BA53 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 32.6% of cic^fet-U6/mirr^6D1 cic^BA53 females exhibit a severe eggshell phenotype (compared to 85.9 in the heterozygote). The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cic^BA53/cic^fet-U6 mutant, indicating that mirr^6D1 suppresses the cic^BA53/cic^fet-U6 phenotype.

Stage 12 grk³/grk^2B6 ; cic^fet-U6/cic^fet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.

rho⁶ mutant clones have no effect on the ectopic appendage-producing fates in cic^fet-U6/cic^fet-T6 mutant egg chambers, indicating that loss of rho function cannot suppress the cic phenotype.

(Atkey et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

cic^Q677X

(Tseng et al., 2007)

cic^R505W

(Tseng et al., 2007)

cic^Q474X

(Tseng et al., 2007)

cic^Q219X

(Tseng et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutant that shows extra wing veins or blisters over cic^fet-E11.

(Goff et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

cic^U6

(Lachance et al., 2009)

cic^fet-U6

cic^fetU6

(Papagianni et al., 2018, Jin et al., 2015, Andreu et al., 2012, Fuchs et al., 2012, Technau et al., 2012, Astigarraga et al., 2007, Tseng et al., 2007, Atkey et al., 2006)

fet^U6

(Goff et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (10)

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