FlyBase Allele Report: Dmel\mus81[Nhe]

General Information

Symbol

Dmel\mus81^Nhe

Species

D. melanogaster

Name

FlyBase ID

FBal0154848

Feature type

allele

Associated gene

Dmel\mus81

Associated Insertion(s)

Carried in Construct

Also Known As

mus81^NheI

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Trowbridge et al., 2007)

I-SceI

(Trowbridge et al., 2007)

FLPase

(Trowbridge et al., 2007)

I-CreI

(Trowbridge et al., 2007)

gene targeting by homologous recombination

(Trowbridge et al., 2007)

Progenitor genotype

Cytology

Description

Insertion of 16bp that contains an NheI site and an in-frame stop codon.

(Trowbridge et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Bellendir et al., 2017)

chemical sensitive

(Bellendir et al., 2017, Trowbridge et al., 2007, Trowbridge et al., 2004)

fertile

(Trowbridge et al., 2007)

viable

(Andersen et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

mus81^Nhe mutants do not show increased sensitivity to larval food exposure to DNA damaging agents camptothecin or methyl methanesulfonate, or to exposure to ionizing radiation, as compared to controls; these mutants show decreased sensitivity to hydroxyurea, with significantly increased adult survival as compared to wild type; and show increased sensitivity to mechlorethamine, with significantly decreased adult survival as compared to wild type.

(Bellendir et al., 2017)

Similarly to wild-type third-instar larvae, X-ray irradiation of mus81^Nhe mutants induces cell cycle arrest in wing imaginal disc cells.

(Kondo and Perrimon, 2011)

mus81^Nhe flies have normal fertility and wild-type levels of X chromosome nondisjunction.

Mutant larvae show moderate hypersensitivity to camptothecin and nitrogen mustard (showing reduced survival to adulthood when fed one of these compounds). Mutants do not show hypersensitivity to UV, MMS or ionising radiation. mus81^Nhe/Y and mus81^Nhe/Df(1)AD11 larvae are less sensitive to hydroxyurea than wild-type larvae.

(Trowbridge et al., 2007)

Sensitive to camptothecin.

(Trowbridge et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Blm^N1/Blm^D2, mus81^Nhe has lethal phenotype, suppressible by spn-A^093A/spn-A^093A

(Trowbridge et al., 2007)

Enhancer of

Statement

Reference

mus81^Nhe/mus81^Nhe is an enhancer of chemical sensitive phenotype of Gen^Z5997/Df(3L)Exel6103

(Bellendir et al., 2017)

mus81^Nhe is an enhancer of increased cell death phenotype of Blm^N1

(Trowbridge et al., 2007)

NOT Enhancer of

Statement

Reference

mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

PolD3^L2/mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

Pif1¹⁶⁷/mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

Suppressor of

Statement

Reference

slx1^F93I/mus81^Nhe is a suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

NOT Suppressor of

Statement

Reference

mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

PolD3^L2/mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

Pif1¹⁶⁷/mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

mus81^Nhe is a non-suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

Other

Statement

Reference

PolD3^L2, mus81^Nhe has viable phenotype

(Kocak et al., 2019)

Pif1¹⁶⁷, mus81^Nhe has viable phenotype

(Kocak et al., 2019)

WRNexo^Δ, mus81^Nhe has short lived phenotype

(Bolterstein et al., 2014)

WRNexo^Δ, mus81^Nhe has partially lethal phenotype

(Bolterstein et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus81^Nhe has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus81^Nhe, slx1^F93I has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus312^D1/mus312^Z1973, mus81^Nhe has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority die phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority live phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus81^Nhe has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Blm^N2/Blm^D2, mus81^Nhe has viable phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus81^Nhe has lethal | pharate adult stage phenotype

(Andersen et al., 2011)

Gen^unspecified/Df(3L)Exel6103, mus81^Nhe has increased cell death phenotype

(Andersen et al., 2011)

mei-9^A1, mus81^Nhe has radiation sensitive phenotype

(Kondo and Perrimon, 2011)

Blm^N1/Blm^D2, mus81^Nhe has lethal phenotype

(Trowbridge et al., 2007)

Blm^D3/Blm^D2, mus81^Nhe has lethal phenotype

(Trowbridge et al., 2007)

Blm^D3/Blm^N1, mus81^Nhe has lethal phenotype

(Trowbridge et al., 2007)

Blm^N2/Blm^N1, mus81^Nhe has viable phenotype

(Trowbridge et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Blm^N1/Blm^D2, mus81^Nhe has condensed chromosome phenotype, suppressible by spn-A^unspecified

(Andersen et al., 2011)

Other

Statement

Reference

Blm^N1/Blm^D2, mus81^Nhe has condensed chromosome phenotype

(Andersen et al., 2011)

Gen^unspecified/Df(3L)Exel6103, mus81^Nhe has wing disc phenotype

(Andersen et al., 2011)

mei-9^A1, mus81^Nhe has wing disc | third instar larval stage phenotype

(Kondo and Perrimon, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

mus81^Nhe/mus81^Nhe exacerbates the sensitivity of Gen^Z5997/Df(3L)Exel6103 mutants to DNA damaging agents camptothecin, methyl methanesulfonate, and hydroxyurea, as well as exacerbating sensitivity to ionizing radiation, as shown by a decrease in adult survival after larval feeding with chemical agents.

(Bellendir et al., 2017)

WRNexo^Δ/WRNexo^Δ mus81^Nhe/mus81^Nhe double mutants survive to adulthood but homozygotes eclose at lower frequencies than expected and show poor survival.

(Bolterstein et al., 2014)

The rate of spontaneous mitotic crossovers in Fancm⁰⁶⁹³/Df(3R)ED6058 males is not affected if animals are also mutant for mus81^Nhe. However crossovers are nearly absent in Fancm⁰⁶⁹³/Df(3R)ED6058 animals that are also mutant for both mus81^Nhe and slx1^F93I.

(Kuo et al., 2014)

Mitotic neuroblasts in mus81^Nhe ; mus309^N1/mus309^D2 double mutant larvae show an increased frequency of broken chromosomes compared to wild type.

The addition of spn-A^unspecified significantly decreases the number of chromosome breaks seen in mitotic neuroblasts of mus81^Nhe ; mus309^N1/mus309^D2 larvae.

(Andersen et al., 2011)

In contrast to mei-9^A1 or mus81^Nhe single mutant larvae, X-ray irradiation of mei-9^A1, mus81^Nhe double mutant larvae induces cell cycle arrest in wing imaginal disc cells.

(Kondo and Perrimon, 2011)

Apoptosis in the wing disc is highly elevated compared to wild type in mus81^Nhe ; mus309^N1 double mutants.

(Trowbridge et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mus1^Nhe1

(Andersen et al., 2011)

mus81^Nhe1

mus81^Nhe

(Carvajal-Garcia et al., 2021, Kocak et al., 2019, Kuo et al., 2014, Kondo and Perrimon, 2011, Trowbridge et al., 2007)

mus81^NheI

(Bellendir et al., 2017, Bolterstein et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (9)

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