Open Close
General Information
Symbol
Dmel\l(3)mbtGM76
Species
D. melanogaster
Name
FlyBase ID
FBal0155050
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G27268154A

Amino acid change:

W928term | l(3)mbt-PB

Reported amino acid change:

W928term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W928term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

l(3)mbtGM76/Df(3R)ED10966 females do not lay eggs; ovaries are atrophied and ovarioles contain fewer individualized egg chambers than l(3)mbtGM76 heterozygous controls; egg chambers occasionally contain apoptotic cells and are highly abnormal, showing follicle layer integrity defects, accumulation of undifferentiated germ cells (i.e. marked by branched fusomes and enclosed by follicle cells), extra nurse cells and oocytes, egg chamber fusion (e.g. occasionally there are multiple germaria connected to the same aberrant egg chamber) and accumulation of stalk cells on top of follicle cells, as compared to l(3)mbtGM76 heterozygous controls; however, similar to controls, mutant germaria present germline stem cells adjacent to the somatic niche.

Similar to controls, l(3)mbtGM76/Df(3R)ED10966 transheterozygous ovaries in L3 have distinct apical compartments harboring terminal filaments, as well as a medial region containing germ cells and intermingled cells; however, there is a decreased number of somatic cells in a similarly sized germ cell containing region and mutant intermingled cells fail to migrate in between the progenitor germline cells and instead surround them.

Egg chambers bearing l(3)mbtGM76 homozygous follicle cell clones show supernumerary nurse cells or multiple oocytes.

l(3)mbtGM76/Df(3R)ED10966 females that also express l(3)mbtUASp.Tag:MYC under the control of Scer\GAL4tj-NP1624 produce embryos that fail to hatch.

The volume of the optic lobe neuroepithelium is significantly increased in size in homozygous and l(3)mbtGM76/Df(3R)D605 third instar larvae compared to wild type. Cell number and mitotic pattern within the optic lobe appears normal in homozygous first and early second instar larvae, but starting in late second instar, the mutant epithelia expand and start folding.

Embryos resulting from l(3)mbtGM76 germ-line clones have reduced numbers of germ cells compared to wild-type embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

l(3)mbtGM76/Df(3R)ED10966 has ovariole phenotype, suppressible | partially by pum[+]/pum680

l(3)mbtGM76/Df(3R)ED10966 has egg chamber phenotype, suppressible | partially by pum[+]/pum680

l(3)mbtGM76/Df(3R)ED10966 has follicle cell phenotype, suppressible | partially by pum[+]/pum680

NOT suppressed by
Statement
Reference

l(3)mbtGM76/Df(3R)ED10966 has ovary phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has ovariole phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has egg chamber phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has nurse cell | supernumerary phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has oocyte | supernumerary phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has follicle cell phenotype, non-suppressible by nosL7/nos[+]

l(3)mbtGM76/Df(3R)ED10966 has ovary phenotype, non-suppressible by aubHN2/aubQC42

Additional Comments
Genetic Interactions
Statement
Reference

The ovariole/egg chamber defects observed in l(3)mbtGM76/Df(3R)ED10966 females are weakly and strongly suppressed by pum680 heterozygosity and homozygosity, respectively.

At 25[o]C temperature, Lint-11/Lint-11, l(3)mbtGM76/+ females are sterile, unlike Lint-11/Lint-11 or l(3)mbtGM76/+ females.

The increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbtGM76 homozygotes is suppressed if the larvae are also homozygous for Df(3L)banΔ1.

Expression of exScer\UAS.cBa under the control of Scer\GAL4C855a suppresses the increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbtGM76 homozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

The egg chamber phenotypes of l(3)mbtGM76/Df(3R)ED10966 females are almost completely rescued (95%) by the expression of l(3)mbtUASp.Tag:MYC under the control of Scer\GAL4tj-NP1624.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

In trans Df(3R)D605 or Df(3R)mbtPE3, l(3)mbt alleles form the following phenotypic series: l(3)mbtGM161 < l(3)mbtts1 < l(3)mbtGM79 < l(3)mbtE2 < l(3)mbtGM76. Phenotypes range from weak maternal-effect mitotic asynchrony to a severe mitotic block to oogenesis defects in trans-heterozygous females. Each of these phenotypes is temperature sensitive: phenotypes increase in severity as the temperature is raised from 18 to 29oC.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dL(3)mbtGM76
l(3)mbtGM76
Name Synonyms
Secondary FlyBase IDs
    References (4)