FlyBase Allele Report: Scer\GAL4[eve.RRa]

General Information

Symbol

Scer\GAL4^eve.RRa

Species

S. cerevisiae

Name

FlyBase ID

FBal0155842

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{eve-GAL4.RRa}

Also Known As

RRa-GAL4, eve-GAL4, RRa(eve)-Gal4, eve[RRa-F]-GAL4, RRa-Gal4-F, RRAGal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fujioka et al., 2003)

Progenitor genotype

Carried in construct

P{eve-GAL4.RRa}

Cytology

Description

eve sequences from +7.9kb to +9.2kb (relative to the transcription start site) are fused upstream of eve promoter sequences from -275bp to +11bp followed by the GAL4 driver coding region. Sequence consisting of a 38bp multi-cloning sequence followed by eve DNA from +91 to +99 nucleotides and an ATG codon has been used to replace the GAL4 translation initiation signal with that of eve. eve 3' sequences from +1306bp to +1521bp are present downstream of the GAL4 coding region.

(Fujioka et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

eve

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval DA1 motor neuron

(Fujioka et al., 2003)

larval RP2 motor neuron

(Fujioka et al., 2003)

third instar larval stage

larval A1-7-innervating DA1 motor neuron

(Fushiki et al., 2016)

larval DA1 motor neuron

(Hasegawa et al., 2016, Zwart et al., 2016, Hartwig et al., 2008)

larval RP2 motor neuron

(Hasegawa et al., 2016, Hartwig et al., 2008)

Additional Information

Statement

Reference

Drives expression in the aCC, RP2, and A1-7 DA1 motor neurons in larvae and adults.

(FlyBase Curators, 2017)

Marker for

larval A1-7-innervating DA1 motor neuron

(Takagi et al., 2017)

Reflects expression of

eve

(Fushiki et al., 2016, Hartwig et al., 2008, Fujioka et al., 2003)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of decreased cell number | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/pum^KK109048 is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^eve.RRa/pum^UAS.cSa is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Scer\GAL4^eve.RRa/nSMase^EY00448 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/nSMase^EY00448 is a suppressor | partially of decreased cell number | third instar larval stage phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of larval RP2 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/CDase^UAS.cAa is an enhancer of larval DA1 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^eve.RRa/pum^KK109048 is a non-enhancer of larval DA1 motor neuron | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Scer\GAL4^eve.RRa/CDase^UAS.cAa is a non-enhancer of autophagosome | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^eve.RRa/pum^UAS.cSa is a suppressor of larval DA1 motor neuron | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Scer\GAL4^eve.RRa/nSMase^EY00448 is a suppressor | partially of larval RP2 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/nSMase^EY00448 is a suppressor | partially of larval DA1 motor neuron | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Scer\GAL4^eve.RRa/nSMase^EY00448 is a suppressor of autophagosome | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^eve.RRa/CDase^UAS.cAa is a non-suppressor of autophagosome | third instar larval stage | decreased number phenotype of Df(2L)cl7/bchs⁵⁸

(Hebbar et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^eve.RRa/wnd^UAS.cCa rescues wnd³

(Hao et al., 2016)

Fails to rescue

Scer\GAL4^eve.RRa/wnd^{S301A.S305A.UAS} fails to rescue wnd³

(Hao et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^RRa

(Wolfram et al., 2012)

Scer\GAL4^eve.RRa

Name Synonyms

Secondary FlyBase IDs

References (28)

Report Sections