FlyBase Allele Report: Dmel\nej[ΔBHQ.UAS]

General Information

Symbol

Dmel\nej^ΔBHQ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175578

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

P{UAS-nej.ΔBHQ}

Key Links

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

(Kumar et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-nej.ΔBHQ}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of nej (encodes amino acids 1-1501).

(Kumar et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nej

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^GMR.PU

(Cutler et al., 2015)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Cutler et al., 2015)

visible, with Scer\GAL4^GMR.PF

(Anderson et al., 2005, Kumar et al., 2004)

visible, with Scer\GAL4^GMR.PF, nej³

(Kumar et al., 2004)

visible, with Scer\GAL4^hs.2sev

(Anderson et al., 2005, Kumar et al., 2004)

Phenotype Manifest In

axon | third instar larval stage, with Scer\GAL4^GMR.PU

(Cutler et al., 2015)

cone cell | ectopic, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

eye, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010, Kumar et al., 2004)

eye, with Scer\GAL4^GMR.PF, nej³

(Kumar et al., 2004)

eye, with Scer\GAL4^GMR.PU

(Cutler et al., 2015)

eye, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

eye disc, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Anderson et al., 2005, Kumar et al., 2004)

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^GMR.PU

(Cutler et al., 2015)

interommatidial bristle, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Anderson et al., 2005, Kumar et al., 2004)

ommatidium, with Scer\GAL4^hs.2sev

(Anderson et al., 2005, Kumar et al., 2004)

photoreceptor cell R3, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R3 | ectopic, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R4, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

photoreceptor cell R7 | ectopic, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

Detailed Description

Statement

Reference

Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a reduction in the size of the adult eye, and aberrant axonal targeting of retinal neurons in third instar larvae, as compared to wild type.

(Cutler et al., 2015)

Scer\GAL4^GMR.PF-mediated expression of nej^{ΔBHQ.Scer\UAS} results in a very severe eye phenotype: the external surface of the eye is flattened and reduced in size.

(Mallik and Lakhotia, 2010)

Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in severe roughening of the adult eye and the presence of non-ommatidial bristles in the region of the eye field. The photoreceptors have undergone high levels of retinal degeneration. Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^hs.2sev results in a moderate rough eye phenotype.

(Anderson et al., 2005)

Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in adults with eyes which are flattened, reduced in size and covered in small bristles. Many adult ommatidia show a severe reduction in the number of photoreceptor cells and this phenotype is also seen in ommatidial clusters in the larval eye disc. Many ommatidial clusters in the pupal eye disc contain an increased number of accessory cone cells. Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^hs.2sev results in eyes which have almost normal external surfaces, with the only obvious defect being the frequent loss or mispositioning of interommatidial bristles. However, cross sections of these eyes reveal several defects in ommatidial assembly. Firstly, many ommatidia contain two R3 cells (indicating that a putative R4 cell has instead adopted the R3 fate). Secondly, at a lower frequency, ommatidia of opposite chirality from wild type are seen (indicating that the R3 and R4 cells have adopted the opposite cell fates). Thirdly, the R4 cells fails to be specified in some ommatidia. Fourthly, in many ommatidia either the R3 or R4 cell has adopted an R7 cell fate, and in some cases both R3 and R4 cells adopt the R7 fate. Finally, in rare cases the presumptive R7 cell fails to differentiate. The severe rough eye phenotype caused by expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry nej³/+.

(Kumar et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of abnormal size | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of increased cell death | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

Suppressor of

Statement

Reference

nej^ΔBHQ.UAS/Scer\GAL4^ey.PH is a suppressor of visible | dominant phenotype of so^D

(Kumar et al., 2004)

Other

Statement

Reference

Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU, nej^ΔBHQ.UAS has lethal - all die before end of P-stage phenotype

(Cutler et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Xrp1^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by CrebA^UAS.cRa

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by rho^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by spi^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Pfas^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Egfr^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Kr^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Ubx^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by cgd^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by dpp^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by eya^D1

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by kni^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, enhanceable by Phm^unspecified

(Anderson et al., 2005)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-enhanceable by Df(2L)dac-4

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-enhanceable by so³

(Anderson et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by ey^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by gl^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by l(3)71DEc^E86

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by l(3)72Df³

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by l(3)L3659^L3659

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by lilli^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by luc^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by rn^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by sty^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by CrebA^unspecified

(Anderson et al., 2005)

Scer\GAL4^hs.2sev, nej^ΔBHQ.UAS has eye phenotype, suppressible by CrebA[+]/CrebA^unspecified

(Anderson et al., 2005)

Scer\GAL4^hs.2sev, nej^ΔBHQ.UAS has eye phenotype, suppressible by CrebB-17A[+]/CrebB^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by Tpl^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, suppressible by enc^unspecified

(Anderson et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-suppressible by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-suppressible by Df(2L)dac-4

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS has eye phenotype, non-suppressible by so³

(Anderson et al., 2005)

Enhancer of

Statement

Reference

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of eye disc | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of eye photoreceptor cell | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔBHQ.UAS, Scer\GAL4^GMR.PU is an enhancer of axon | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

Suppressor of

Statement

Reference

nej^ΔBHQ.UAS/Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of so^D

(Kumar et al., 2004)

nej^ΔBHQ.UAS/Scer\GAL4^ey.PH is a suppressor of eye phenotype of so^D

(Kumar et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^ey.PH suppresses the no-eye phenotype of so^D/+, to the same degree as expression of nej^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^ey.PH.

(Kumar et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of nej^{ΔBHQ.Scer\UAS} enhances the eye phenotype of adult flies, and enhances the increased cell death and aberrant axonal targeting observed in third instar eye disc of flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU, and results in failure of these flies to eclose.

(Cutler et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nej^{ΔBHQ.Scer\UAS}

nej^ΔBHQ.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

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