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General Information
Symbol
Scer\GAL4c205
Species
S. cerevisiae
Name
FlyBase ID
FBal0190689
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
c205-GAL4, c205
Allele class
Mutagen
    Nature of the Allele
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference

    Scer\GAL4c205 drives expression in layer 5 of the fan-shaped body, co-localising with Mip protein.

    Expression of Scer\GAL4c205 was seen in eight to ten fan-shaped F5 neurons with somata that are loosely arranged near the calyx of the mushroom body at each side of the protocerebrum. The primary neurites of these cells converge into a bundle that runs ventrally and anteriorly into the superior medial protocerebrum.

     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Acute inhibition (using shi1.UAS at 30[o]C) of a subset of Scer\GAL4c205 cells (expression restricted using Scer\GAL80Mef2.mb247) in adults does not affect electric shock avoidance or avoidance of higher temperatures, compared to controls, and there is no effect on locomotion activity index; inhibition of this subset during testing alone, but not training alone, significantly reduces short-term aversive, but not appetitive, olfactory memory, compared to controls.

    Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4c205 cells, in combination with a Gal80[ts] transgene to begin expression three days prior to experiments, in adults does not affect electric shock avoidance, compared to controls, and there is no effect on locomotion activity index.

    Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.UAS.Citrine with (orange) light) of Scer\GAL4c205 cells in one chamber of a T-maze does not significantly increase in avoidance of that chamber, compared to controls, and there is no effect on locomotion activity index.

    Thermogenetic activation (using TrpA1UAS.(B).cKa at 26[o]C) of a subset of Scer\GAL4c205 cells (expression restricted using Scer\GAL80Mef2.mb247) in adults has no effect on locomotion activity index, compared to controls.

    Thermogenetic activation (using TrpA1UAS.(B).cKa at 26[o]C) of a subset of Scer\GAL4c205 cells (expression restricted using Scer\GAL80Mef2.mb247) in adults, in combination with a paired odor, does not affect aversive olfactory memory, compared to controls, and there is no effect on locomotion activity index.

    Inhibition of neural activity in Scer\GAL4c205-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

    Activation of fan-shaped body dopaminergic neurons through overexpression of temperature sensitive TrpA1 under the control of Scer\GAL4c205 produces a large increase in the amount of sleep seen in mature (7-8 day old) flies, but has a less severe effect on young (1 day old) flies.

    Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4c205 cells in adults significantly reduces short-term aversive visual, but not olfactory, unimodal memory, compared to controls; inhibition does not affect short-term aversive memory for tested olfactory cues that are associated with conditioned visual cues, or for tested visual cues that are associated with conditioned olfactory cues, compared to controls.

    Inhibition (using shi1.UAS at 31[o]C) of the combined set of Scer\GAL4Mef2.247 cells and Scer\GAL4c205 cells in adults significantly reduces short-term aversive memory for tested visual cues that are associated with conditioned olfactory cues, and for tested olfactory cues that are associated with conditioned visual cues, compared to controls.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
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    Additional Comments
    Genetic Interactions
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    Reference
    Xenogenetic Interactions
    Statement
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    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Scer\GAL4c205
    Name Synonyms
    Secondary FlyBase IDs
      References (22)