FlyBase Allele Report: Scer\GAL4[S3.Hug]

General Information

Symbol

Scer\GAL4^S3.Hug

Species

S. cerevisiae

Name

FlyBase ID

FBal0198094

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Hug-GAL4.S3}

Also Known As

hugin-Gal4, HugS3-GAL4, hug-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Melcher and Pankratz, 2005)

Progenitor genotype

Carried in construct

P{Hug-GAL4.S3}

Cytology

Description

A 1.5kb Hug promoter fragment regulates expression of a GAL4 driver.

(Melcher and Pankratz, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

Hug

Hug_S3

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval corpus cardiacum-innervating Hugin neuron

(Melcher and Pankratz, 2005)

larval protocerebrum-innervating Hugin neuron

(Melcher and Pankratz, 2005)

larval VNC-innervating Hugin neuron

(Melcher and Pankratz, 2005)

larval pharynx-innervating Hugin neuron

(Melcher and Pankratz, 2005)

adult stage

adult Hugin neuron

(King et al., 2017, Melcher and Pankratz, 2005)

Additional Information

Statement

Reference

Drives expression in Hugin neurons.

(FlyBase Curators, 2017)

Marker for

adult Hugin neuron

(Martelli et al., 2017)

Reflects expression of

Hug

(King et al., 2017, Martelli et al., 2017, Melcher and Pankratz, 2005)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior | adult stage, with Dh44^{UAS.Tag:SS(trypsin),Tag:MYC,Tag:GPI(lynx1)}

(King et al., 2017)

abnormal circadian behavior | adult stage, with Dh44-R1^DsRed, Dh44-R1^JF03208

(King et al., 2017)

abnormal circadian behavior | adult stage, with Dh44-R1^KK108591

(King et al., 2017)

abnormal circadian behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(King et al., 2017)

abnormal circadian behavior | adult stage, with Hug^GD12474

(King et al., 2017)

abnormal circadian behavior | adult stage, with Hug^JF03122

(King et al., 2017)

abnormal circadian behavior | adult stage, with rpr^UAS.C

(King et al., 2017)

abnormal feeding behavior | larval stage, with Ctet\tetX^TNT-LC.UAS

(Melcher and Pankratz, 2005)

abnormal feeding behavior | larval stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Schlegel et al., 2016)

abnormal neurophysiology | larval stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Schlegel et al., 2016)

decreased rate of movement | adult stage, with Hsap\KCNJ2^UAS.EGFP

(King et al., 2017)

Phenotype Manifest In

embryonic/larval antennal nerve | heat sensitive, with TrpA1^UAS.(B).cKa

(Schlegel et al., 2016)

pharyngeal muscle cell | heat sensitive, with TrpA1^UAS.(B).cKa

(Schlegel et al., 2016)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^UAS.EGFP) or ablation (using rpr^UAS.C) of Scer\GAL4^S3.Hug cells in adults significantly reduces the strength of circadian locomotor rhythms under constant darkness, compared to controls; inhibition (using Hsap\KCNJ2^UAS.EGFP) significantly reduces evening peak activity under light-dark and dark-dark conditions, compared to controls, with little effect on feeding rhythms.

(King et al., 2017)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^S3.Hug cells in larvae reduces food intake and pharyngeal pump cycle frequency (antennal nerve activity and cibarial dilator musculature contractions), compared to controls.

(Schlegel et al., 2016)

Ablation (using rpr^UAS.cUa) of Scer\GAL4^S3.Hug cells in adult males does not affect food intake, compared to controls.

(Al-Anzi et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^S3.Hug, TrpA1^UAS.(B).cKa has abnormal feeding behavior | larval stage | heat sensitive phenotype, suppressible | partially by Hug^RNAi.UAS.1A, Scer\GAL4^S3.Hug