Flies expressing Atg9GD2029 under the control of Scer\GAL4Mef2.PU display progressive heart defects: thicker heart walls and a distorted lumen with a rough surface in older flies, increased systolic dimension throughout adult life, but no change in diastolic dimension and fractional shortening. Mitochondria in cardiomyocytes are abnormally elongated and narrowed, the mitochondrial network appears fused/enlarged and the mitochondrial volume density is significantly increased in 40-day old flies. Alongside, indirect flight muscles display fragmented mitochondria and reduced mitochondrial volume density. Neither males nor females display climbing defects.
Adults expressing Atg9GD2029 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.