FlyBase Allele Report: Dmel\ND-75[GD6309]

General Information

Symbol

Dmel\ND-75^GD6309

Species

D. melanogaster

Name

FlyBase ID

FBal0207698

Feature type

allele

Associated gene

Dmel\ND-75

Associated Insertion(s)

Carried in Construct

P{GD6309}

Also Known As

ND75 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6309}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6309

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of mitochondrial metabolism disease

CEA

(Hegde et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Hegde et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^da.PU

(Hegde et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^da.PU

(Hegde et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^repo.PU

(Hegde et al., 2014)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

increased cell death, with Scer\GAL4^da.PU

(Hegde et al., 2014)

increased cell number | third instar larval stage, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

short lived, with Scer\GAL4^da.PU

(Hegde et al., 2014)

short lived, with Scer\GAL4^elav.PU

(Hegde et al., 2014)

short lived, with Scer\GAL4^repo.PU

(Hegde et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult brain, with Scer\GAL4^da.PU

(Hegde et al., 2014)

adult brain, with Scer\GAL4^repo.PU

(Hegde et al., 2014)

lamellocyte | larval stage, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

lamellocyte | larval stage, with Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

lamellocyte | third instar larval stage, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

mitochondrion, with Scer\GAL4^da.PU

(Hegde et al., 2014)

mitochondrion, with Scer\GAL4^repo.PU

(Hegde et al., 2014)

Detailed Description

Statement

Reference

Flies expressing ND75^GD6309 under the control of Scer\GAL4^da.PU exhibit progressive neurodegeneration: no neuron loss is seen at one day, but significant cortical neuron loss is observed at 10 days old. An increased amount of cell death (caspase activation) is seen compared to controls, and the majority of these cells are neurons rather than glia. The mitochondria of cortical cells are enlarged compared to controls, and both neurons and glia are affected. At 30 days, the number and size of the mitochondria is dramatically increased, sometimes to the extent of almost filling the cytoplasm. Mitochondrial defects observed include honeycombing, effacement of cristae and concentric lamellations of the internal mitochondrial membrane.

When ND75^GD6309 is expressed in neurons under the control of Scer\GAL4^elav.PU no neuronal loss, vacuolation or mitochondrial enlargement is observed. However the lifespan of these flies is significantly shortened and climbing dysfunction is observed in five day old flies.

When ND75^GD6309 is expressed in glia under the control of Scer\GAL4^repo.PU neuronal loss, cortical vacuolation and diffuse mitochondrial enlargement are observed. Lifespan is reduced but not climbing defects are seen compared to controls.

(Hegde et al., 2014)

Expression of ND75^GD6309 in the posterior signalling center (PSC) under the control of either Scer\GAL4^Antp-10 leads to an increase in the number of circulating lamellocytes. Differentiation of crystal cells and plasmatocytes is unaffected. Lamellocyte number is unaffected when ND75^GD6309 is expressed under the control of Scer\GAL4^Lsp2.PH (fat body), Scer\GAL4^A58 (epidermis), Scer\GAL4^c127 (neurons), Scer\GAL4^HCH.Hand (dorsal vessel), Scer\GAL4⁵⁰¹⁵ (ring gland), Scer\GAL4^ap-md544 (wing disc) or Scer\GAL4^btl.PS (trachea). Autonomous expression in the lymph gland progenitor cells under the control of Scer\GAL4^dome-PG14 also has no effect on lamellocyte differentiation.

(Sinenko et al., 2011)

Expression of ND75^GD6309 in the posterior signalling center (PSC) under the control of either Scer\GAL4^Antp-10 or Scer\GAL4^Dot.PK leads to an increase in the number of circulating lamellocytes. The number of PSC cells is unchanged when ND75^GD6309 is expressed under the control of Scer\GAL4^Antp-10, and no apoptosis is detected.

(Sinenko et al., 2011)

Adults expressing ND-75^GD6309 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

ND-75^GD6309, Scer\GAL4^Antp-10 has increased cell number | third instar larval stage phenotype, non-suppressible by upd2^NIG.5988R, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has increased cell number | third instar larval stage phenotype, non-suppressible by upd1^NIG.5993R, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has increased cell number | third instar larval stage phenotype, non-suppressible by upd3^RNAi.UAS, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has increased cell number | third instar larval stage phenotype, non-suppressible by egr^KK103432, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has increased cell number | third instar larval stage phenotype, non-suppressible by egr^GD12658, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | larval stage phenotype, suppressible by Sod2^UAS.cMa, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Ugt36A1.PK has lamellocyte | larval stage phenotype, suppressible by foxo^UAS.cPa, Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Ugt36A1.PK has lamellocyte | larval stage phenotype, suppressible by Akt^GD1361, Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Ugt36A1.PK has lamellocyte | larval stage phenotype, suppressible by Akt^KK100495, Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Ugt36A1.PK has lamellocyte | larval stage phenotype, suppressible by Scer\GAL4^Antp-10/spi^NIG.10334R

(Sinenko et al., 2011)

NOT suppressed by

Statement

Reference

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | third instar larval stage phenotype, non-suppressible by upd2^NIG.5988R, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | third instar larval stage phenotype, non-suppressible by upd1^NIG.5993R, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | third instar larval stage phenotype, non-suppressible by upd3^RNAi.UAS, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | third instar larval stage phenotype, non-suppressible by egr^KK103432, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

ND-75^GD6309, Scer\GAL4^Antp-10 has lamellocyte | third instar larval stage phenotype, non-suppressible by egr^GD12658, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of upd2^NIG.5988R does not suppress the increase in circulating lamellocytes seen when ND-75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

Expression of upd1^NIG.5993R does not suppress the increase in circulating lamellocytes seen when ND-75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

Expression of upd3^{dsRNA.Scer\UAS} does not suppress the increase in circulating lamellocytes seen when ND-75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

Expression of egr^KK103432 does not suppress the increase in circulating lamellocytes seen when ND-75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

Expression of egr^GD12658 does not suppress the increase in circulating lamellocytes seen when ND-75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

(Sinenko et al., 2011)

Expression of Sod2^Scer\UAS.cMa suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

Expression of foxo^Scer\UAS.cPa suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Dot.PK.

Expression of Akt1^GD1361 suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Dot.PK.

Expression of Akt1^KK100495 suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Dot.PK.

Expression of spi^NIG.10334R suppresses the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

(Sinenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Down-regulation of ND75 through expression of ND75^GD6309 in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS necrotic neurons enhances ROS production and activated JNK signaling.

(Yang et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v52047

w¹¹¹⁸; P{GD6309}v52047

v52049

w¹¹¹⁸; P{GD6309}v52049