FlyBase Allele Report: Dmel\Nav[A384]

General Information

Symbol

Dmel\Nav^A384

Species

D. melanogaster

Name

FlyBase ID

FBal0217029

Feature type

allele

Associated gene

Dmel\Nav

Associated Insertion(s)

PBac{5HPw⁺}Nav^A384

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Abe et al., 2014)

hypomorphic allele - molecular evidence

(Abe et al., 2014)

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{5HPw⁺}Nav^A384

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:19,924,842..19,924,842 [-]

(Abe et al., 2014, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Nav^Δ)

(Abe et al., 2014)

abnormal neuroanatomy (with Nav^Δ), with Nav^UAS.cAa, Scer\GAL4^elav.PU

(Abe et al., 2014)

abnormal neuroanatomy (with Nav^Δ-L1)

(Abe et al., 2014)

abnormal neuroanatomy (with Nav^Δ-L2)

(Abe et al., 2014)

abnormal neuroanatomy (with Nav^Δ-L3)

(Abe et al., 2014)

Phenotype Manifest In

adult mushroom body alpha-lobe (with Nav^Δ)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with Nav^Δ-L1)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with Nav^Δ-L2)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with Nav^Δ-L3)

(Abe et al., 2014)

adult mushroom body beta-lobe (with Nav^Δ)

(Abe et al., 2014)

adult mushroom body beta-lobe (with Nav^Δ), with Nav^UAS.cAa, Scer\GAL4^elav.PU

(Abe et al., 2014)

adult mushroom body beta-lobe (with Nav^Δ-L1)

(Abe et al., 2014)

adult mushroom body beta-lobe (with Nav^Δ-L2)

(Abe et al., 2014)

adult mushroom body beta-lobe (with Nav^Δ-L3)

(Abe et al., 2014)

Detailed Description

Statement

Reference

sick^A384/sick^Δ mutant mushroom bodies display short α/β axonal lobes compared with those of controls, although the morphology of the peduncle appears normal. In addition, sick^A384/sick^Δ mutants display defective ellipsoid body ring structures in each hemisphere of the central brain. A prominent increase in F-actin signal is detected in the core region of the defective α/β axonal lobe compared to controls.

sick^A384/sick^Δ-L1 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^A384/sick^Δ-L2 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^A384/sick^Δ-L3 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

No axonal lobe phenotypes are observed in the mushroom bodies of sick^A384/+ mutants.

(Abe et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nav^A384/Nav^Δ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

Nav^A384/Nav^Δ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has abnormal neuroanatomy phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

NOT suppressed by

Statement

Reference

Nav^A384/Nav^Δ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Rac1^{Y40C.UAS.Tag:MYC}, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nav^A384/Nav^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

NOT suppressed by

Statement

Reference

Nav^A384/Nav^Δ has adult mushroom body alpha-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body beta-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body alpha-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

Nav^A384/Nav^Δ has adult mushroom body beta-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Rac1^{Y40C.UAS.Tag:MYC}, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Rac1^{Y40C.UAS.Tag:MYC}, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Rac1^{Y40C.UAS.Tag:MYC}, Nav^A384, Nav^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of tsr^S3E.Scer\UAS under the control of Scer\GAL4^NP7175 does not significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^NP7175 partially suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{CS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^NP7175 does not significantly suppress the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{Scer\UAS.T:Ivir\HA1} suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. This suppression does not occur in a sick^Δ/sick^A384 mutant background.

Expression of Rac1^{Y40C.Scer\UAS.T:Hsap\MYC} suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. This suppression does not occur in a sick^Δ/sick^A384 mutant background.

(Abe et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^elav.PU/Nav^UAS.cAa

(Abe et al., 2014)

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^ey-OK107/Nav^UAS.cAa

(Abe et al., 2014)

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^NP7175/Nav^UAS.cAa

(Abe et al., 2014)

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^elav.PU/Nav^UAS.mCherry

(Abe et al., 2014)

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^ey-OK107/Nav^UAS.mCherry

(Abe et al., 2014)

Nav^A384/Nav^Δ is partially rescued by Scer\GAL4^NP7175/Nav^UAS.mCherry

(Abe et al., 2014)

Not rescued by

Nav^A384/Nav^Δ is not rescued by Scer\GAL4^ey-OK107/Nav^{K1881A.UAS.mCherry}

(Abe et al., 2014)

Nav^A384/Nav^Δ is not rescued by Scer\GAL4^NP7175/Nav^{K1881A.UAS.mCherry}

(Abe et al., 2014)

Comments

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^elav.PU almost fully rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants. The rescued flies exhibit a β lobe fusion phenotype.

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^ey-OK107 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^NP7175 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav.PU almost fully rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ey-OK107 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^NP7175 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{K1881A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ey-OK107 fails to rescue the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{K1881A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^NP7175 fails to rescue the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

(Abe et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

16102

y¹ w¹¹¹⁸; PBac{5HPw⁺}Nav^A384/CyO

Kyoto

114425

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; PBac{5HPw⁺}Nav^A384 P{neoFRT}40A/CyO y⁺

Notes on Origin