FlyBase Allele Report: Dmel\Stim[RNAi.cEa.UAS]

General Information

Symbol

Dmel\Stim^RNAi.cEa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240695

Feature type

allele

Associated gene

Dmel\Stim

Associated Insertion(s)

Carried in Construct

P{UAS-Stim.RNAi.E}

Key Links

Transgenic product class

RNAi_reagent

(Eid et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Eid et al., 2008)

Mutagen

in vitro construct

(Eid et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Stim.RNAi.E}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of Stim sequences (composed of a genomic fragment and a cDNA fragment corresponding to the same region of Stim).

(Eid et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Stim

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Xu et al., 2019, Baumbach et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal adult locomotory behavior | conditional, with Scer\GAL4^Mef2.PR

(Nelson et al., 2019)

abnormal feeding behavior | adult stage, with Scer\GAL4^fat

(Xu et al., 2019)

abnormal feeding behavior | adult stage | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Xu et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^fat

(Xu et al., 2019)

abnormal size | adult stage | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

abnormal starvation stress response | adult stage, with Scer\GAL4^fat

(Xu et al., 2019)

decreased rate of movement | larval stage, with Scer\GAL4^da.G32

(Eid et al., 2008)

decreased rate of movement | larval stage, with Scer\GAL4^e22c

(Eid et al., 2008)

increased cell size | adult stage | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

lethal, with Scer\GAL4^αTub84B.PL

(Nelson et al., 2019)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Eid et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^e22c

(Eid et al., 2008)

long lived, with Scer\GAL4^fat

(Xu et al., 2019)

long lived | calorie restriction conditional, with Scer\GAL4^fat

(Xu et al., 2019)

some die during larval stage, with Scer\GAL4^da.G32

(Eid et al., 2008)

some die during larval stage, with Scer\GAL4^e22c

(Eid et al., 2008)

viable, with Scer\GAL4^Mef2.PR

(Nelson et al., 2019)

visible, with Df(1)sd72a, Scer\GAL4^en-e16E

(Eid et al., 2008)

visible, with Scer\GAL4^Bx-MS1096

(Eid et al., 2008)

visible, with Scer\GAL4^en-e16E

(Eid et al., 2008)

visible, with Scer\GAL4^pnr-MD237

(Eid et al., 2008)

visible, with Scer\GAL4^sd-SG29.1

(Eid et al., 2008)

Phenotype Manifest In

adult abdomen | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

adult abdominal fat mass | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

embryonic/larval fat body, with Scer\GAL4^da.G32

(Eid et al., 2008)

embryonic/larval fat body, with Scer\GAL4^e22c

(Eid et al., 2008)

fat cell | adult stage | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

lipid droplet | adult stage | progressive, with Scer\GAL4^fat

(Xu et al., 2019)

mesonotum, with Scer\GAL4^pnr-MD237

(Eid et al., 2008)

microchaeta & mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Eid et al., 2008)

wing vein, with Df(1)sd72a, Scer\GAL4^en-e16E

(Eid et al., 2008)

wing vein, with Scer\GAL4^Bx-MS1096

(Eid et al., 2008)

wing vein, with Scer\GAL4^en-e16E

(Eid et al., 2008)

wing vein, with Scer\GAL4^sd-SG29.1

(Eid et al., 2008)

Detailed Description

Statement

Reference

Pulse-induced expression of Stim^{dsRNA.cEa.UAS} in 6 days old adults for 2 days, under the combined control of Scer\GAL4^fat and GAL80[ts], induces progressive increases in abdominal girth, abdominal fat mass, fat body cell size and fat cell lipid droplet content, as well as increased body weight, as compared to controls; these adults exhibit increased food intake, decreased climbing activity, and decreased lifespan under standard nutrition but increased resistance to starvation, as compared to controls; the fat body does not show changes in replication (EdU labelling/new DNA synthesis), as compared to controls.

Pulse-induced expression of Stim^{dsRNA.cEa.UAS} in 4 days old adults for 4 days, under the control of Scer\GAL4^da.Switch.PT and RU-486 feeding, induces increased food intake, as compared to controls.

(Xu et al., 2019)

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of either Scer\GAL4^da.G32 or Scer\GAL4^e22c results in late second to early third instar lethality. The larvae appear contracted and are not highly active, but they are normal in size, with normal patterning of denticle belts. The larvae show transparency of fat body cells.

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^ey.PH, Scer\GAL4^GMR.PF, Scer\GAL4^lz-gal4 or Scer\GAL4^sca.PU does not result in defects in eye development.

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 results in a partially bald notum, with loss of mechanosensory bristles; microchaetae are missing from the domain of highest pnr expression, while macrochaetae are largely unaffected.

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096, Scer\GAL4^sd-SG29.1 or Scer\GAL4^en-e16E results in thickened wing veins.

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^en-e16E in a Df(1)sd72a hemizygous background results in enhanced wing vein thickening compared to expression in a wild-type background.

(Eid et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, Stim^RNAi.cEa.UAS has visible phenotype, enhanceable by Df(1)N-81k1

(Eid et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^da.Switch.PT, Stim^RNAi.cEa.UAS has abnormal feeding behavior | adult stage | RU486 conditional phenotype, suppressible by Akh^A/Akh^A

(Xu et al., 2019)

Scer\GAL4^en-e16E, Stim^RNAi.cEa.UAS has visible phenotype, suppressible by N^TN.ΔBRV.UAS, Scer\GAL4^en-e16E

(Eid et al., 2008)

Suppressor of

Statement

Reference

Stim^RNAi.cEa.UAS, Scer\GAL4^Act5C.PI is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, emei¹

(Ma et al., 2020)

Stim^RNAi.cEa.UAS, Scer\GAL4^en-e16E is a suppressor of visible phenotype of N^TN.ΔBRV.UAS, Scer\GAL4^en-e16E

(Eid et al., 2008)

NOT Suppressor of

Statement

Reference

Stim^RNAi.cEa.UAS, Scer\GAL4^Act5C.PI is a non-suppressor of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, Stim^RNAi.cEa.UAS has wing vein phenotype, enhanceable by Df(1)N-81k1

(Eid et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, Stim^RNAi.cEa.UAS has wing vein phenotype, suppressible by N^TN.ΔBRV.UAS, Scer\GAL4^en-e16E

(Eid et al., 2008)

Suppressor of

Statement

Reference

Stim^RNAi.cEa.UAS, Scer\GAL4^Act5C.PI is a suppressor of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, emei¹

(Ma et al., 2020)

Stim^RNAi.cEa.UAS, Scer\GAL4^en-e16E is a suppressor of wing vein L5 phenotype of N^TN.ΔBRV.UAS, Scer\GAL4^en-e16E

(Eid et al., 2008)

NOT Suppressor of

Statement

Reference

Stim^RNAi.cEa.UAS, Scer\GAL4^Act5C.PI is a non-suppressor of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^en-e16E in a Df(1)N-81k1 background results in severe enhancement of wing vein 4 and 5 thickening compared to expression in a wild-type background.

Co-expression of N^{TN.ΔBRV.Scer\UAS} and Stim^{dsRNA.cEa.Scer\UAS} under the control of Scer\GAL4^en-e16E results in mutual suppression of the single mutant wing vein phenotypes.

(Eid et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Stim^RNAi.cEa.UAS is rescued by Scer\GAL4^en-e16E/Stim^UAS.cEa

(Eid et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

41758

w^*; P{UAS-Stim.RNAi.E}26-1

41759

w^*; P{UAS-Stim.RNAi.E}8-4/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

RNAi^26-1

(Nelson et al., 2019)

RNAi^8-4

(Nelson et al., 2019)

Stim^RNAi.cEa.UAS

Stim^{dsRNA.cEa.Scer\UAS}

Stim^{dsRNA.cEa.UAS}

dStim^RNAi#2

(Weiss et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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