FlyBase Allele Report: Hsim\VP16[AD-VGlut1-OK371]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Hsim\VP16^{AD-VGlut1-OK371}

General Information

Symbol

Hsim\VP16^{AD-VGlut1-OK371}

Species

S. Herpes simplex virus Type 1

Name

FlyBase ID

FBal0243326

Feature type

allele

Associated gene

Associated Insertion(s)

P{dVP16AD}VGlut1^{OK371-dVP16AD}

Carried in Construct

Also Known As

OK371-dVP16AD, vGlut^dVP16-AD, vGlut-dVP16AD, OK371-VP16AD

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Gao et al., 2008)

gene conversion

(Gao et al., 2008)

Progenitor genotype

P{GawB}VGlut1^OK371

(Gao et al., 2008)

Associated Insertion(s)

P{dVP16AD}VGlut1^{OK371-dVP16AD}

Cytology

Description

The P{GawB} element present in the progenitor insertion has been replaced with a P{dVP16AD} element.

(Gao et al., 2008)

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8 -- P15

distal medullary amacrine neuron Dm8a

Comment: when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014)

lamina monopolar neuron L1

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Bahl et al., 2015, Gao et al., 2008)

transmedullary neuron Tm5c

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-Gal4.C1a.DBD}

when combined with P{ort-Gal4.C1a.DBD} (combination referred to as 'ortC1a∩ ok371-dVP16')

(Sancer et al., 2019, Melnattur et al., 2014, Gao et al., 2008)

adult optic lobe neuron | subset

Comment: when combined with P{ort-GAL4.DBD.C1-3}

(Gao et al., 2008)

distal medullary amacrine neuron Dm8a | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

glial cell | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

adult doublesex aDN neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

when combined with TI{GAL4(DBD)::Zip-}dsx^GAL4-DBD

(Nojima et al., 2021, Pavlou et al., 2016)

glutamatergic neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

motor neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

prothoracic leg taste bristle chemosensory neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

distal medullary amacrine neuron Dm8a

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014, Gao et al., 2008)

adult stage | female

adult glutamatergic doublesex AbN (female) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult stage | male

adult glutamatergic doublesex AbN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

transmedullary neuron Tm5c

Comment: when combined with P{ort-LexA.C1a.DBD}

(Karuppudurai et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

(Gao et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal copulation, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^dsx-GAL4-DBD

(Pavlou et al., 2016)

abnormal copulation | male, with GABA-B-R2^GD699, Scer\GAL4^dsx-GAL4-DBD

(Pavlou et al., 2016)

abnormal copulation | male | heat sensitive, with Scer\GAL4^dsx-GAL4-DBD, TrpA1^UAS.(B).cKa

(Pavlou et al., 2016)

abnormal courtship behavior | male, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^dsx-GAL4-DBD

(Pavlou et al., 2016)

abnormal courtship behavior | male | heat sensitive, with Scer\GAL4^dsx-GAL4-DBD, TrpA1^UAS.(B).cKa

(Pavlou et al., 2016)

abnormal neuroanatomy, with Scer\GAL4^DBD.ort.C2, babo^Q302D.UAS

(Ting et al., 2014)

abnormal phototaxis, with Ctet\tetX^{TNT-LC.13x.lexAop.Tag:HA}, Ecol\lexA^DBD.C1a.ort

(Karuppudurai et al., 2014)

abnormal phototaxis, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.C1a.ort

(Karuppudurai et al., 2014)

abnormal phototaxis, with Ecol\lexA^DBD.C1a.ort, clumsy^JF02987

(Karuppudurai et al., 2014)

abnormal phototaxis, with Ecol\lexA^DBD.C1a.ort, shi^{ts1.IVS.p10.20xUAS}

(Karuppudurai et al., 2014)

abnormal phototaxis, with Ecol\lexA^DBD.C1a.ort, VGlut1^JF02689

(Karuppudurai et al., 2014)

male sterile, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^dsx-GAL4-DBD

(Pavlou et al., 2016)

Phenotype Manifest In

distal medullary amacrine neuron Dm8a, with Scer\GAL4^DBD.ort.C2, babo^Q302D.UAS

(Ting et al., 2014)

Detailed Description

Statement

Reference

Blocking synaptic vesicle exocytosis (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the combination of Scer\GAL4^dsx-GAL4-DBD and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT} results in a significant drop in male courtship behavior (they display the normal complement of courtship behaviors including attempts to copulate but spend less time courting target females), fertility (they produce no progeny), and no successful matings occur (males fail to achieve genital coupling) compared to genetic controls.

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 31[o]C) of neurons targeted by the Split-GAL4 combination of Scer\GAL4^dsx-GAL4-DBD and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT} results in a significant drop in male courtship behavior (they display the normal complement of courtship behaviors but spend less time courting target females and never successfully copulate), and no successful matings occur compared to genetic or temperature controls. Thermogenetic activation of these neurons 5 minutes into copulation (when fertilization is not complete) prevents the male from detaching from the female and terminating copulation; decapitation of these males in copulo does not eliminate the 'stuck' phenotype.

(Pavlou et al., 2016)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 sets of Scer\GAL4^DBD.C1a.ort, Hsim\VP16^{AD-VGlut-OK371} and Hsim\VP16^AD-ET24g, or Scer\GAL4^DBD.C1a.ort, Hsim\VP16^AD-ET9A and Hsim\VP16^{AD-VGlut-OK371}, in adults does not affect short-term aversive visual (blue/green) memory, compared to controls.

(Melnattur et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987 has abnormal phototaxis phenotype, non-enhanceable by Ecol\lexA^DBD.C1a.ort/Grik^JF01840, Hsim\VP16^{AD-VGlut1-OK371}

(Karuppudurai et al., 2014)

Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987 has abnormal phototaxis phenotype, non-enhanceable by Ecol\lexA^DBD.C1a.ort, ukar^JF03425, Hsim\VP16^{AD-VGlut1-OK371}

(Karuppudurai et al., 2014)

Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987 has abnormal phototaxis phenotype, non-enhanceable by Ecol\lexA^DBD.C1a.ort/KaiR1D^JF01873, Hsim\VP16^{AD-VGlut1-OK371}

(Karuppudurai et al., 2014)

Enhancer of

Statement

Reference

ort^{UAS.2x.Tag:HA}, Scer\GAL4^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371} is an enhancer of abnormal phototaxis phenotype of HisCl1¹³⁴, ninaE¹, ort¹

(Karuppudurai et al., 2014)

ort^{UAS.2x.Tag:HA}, Scer\GAL4^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371} is an enhancer of abnormal phototaxis phenotype of HisCl1¹³⁴, ninaE¹, ort⁴/ort¹

(Karuppudurai et al., 2014)

ort^{UAS.2x.Tag:HA}, Scer\GAL4^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371} is an enhancer of abnormal phototaxis phenotype of HisCl1¹³⁴, ninaE¹, ort⁶/ort¹

(Karuppudurai et al., 2014)

NOT Enhancer of

Statement

Reference

Ecol\lexA^DBD.C1a.ort/KaiR1D^JF01873, Hsim\VP16^{AD-VGlut1-OK371} is a non-enhancer of abnormal phototaxis phenotype of Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987

(Karuppudurai et al., 2014)

Ecol\lexA^DBD.C1a.ort/Grik^JF01840, Hsim\VP16^{AD-VGlut1-OK371} is a non-enhancer of abnormal phototaxis phenotype of Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987

(Karuppudurai et al., 2014)

ukar^JF03425, Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371} is a non-enhancer of abnormal phototaxis phenotype of Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}, clumsy^JF02987

(Karuppudurai et al., 2014)

Suppressor of

Statement

Reference

Hsim\VP16^{AD-VGlut1-OK371}, babo^Q302D.UAS, Scer\GAL4^DBD.ort.C2 is a suppressor of abnormal neuroanatomy phenotype of sev⁴

(Ting et al., 2014)

Other

Statement

Reference

Ecol\lexA^DBD.C1a.ort, Grik^JF01840, Hsim\VP16^{AD-VGlut1-OK371}, KaiR1D^KK102490, ukar^JF03425 has abnormal phototaxis phenotype

(Karuppudurai et al., 2014)

Ecol\lexA^DBD.C1a.ort, Grik^JF01840, Hsim\VP16^{AD-VGlut1-OK371}, KaiR1D^JF01873, ukar^KK105030 has abnormal phototaxis phenotype

(Karuppudurai et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsim\VP16^{AD-VGlut1-OK371}, babo^Q302D.UAS, Scer\GAL4^DBD.ort.C2 is a suppressor of distal medullary amacrine neuron Dm8a phenotype of sev⁴

(Ting et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Hsim\VP16^{AD-VGlut1-OK371}, ort^UAS.cWa, Scer\GAL4^DBD.ort.C1-3 rescues ort⁴/ort¹

(Gao et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Comments

Used in combination with Ecol\lexA^{C1a.DBD.ort.T:Zzzz\Zip-} as Tm5c-Gal4.

(Macpherson et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Hsim\VP16^{AD-VGlut-OK371}

Hsim\VP16^{AD-VGlut1-OK371}

Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\Zip+,T:SV40\nls-largeT}

Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2}

Hsim\VP16^{VGlut-OK371-dVP16AD}

VGlut^dVP16.AD

(Nojima et al., 2021)

vGlut^dVP16-AD

(Massey et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections