FlyBase Allele Report: Dmel\Acn[27]

General Information

Symbol

Dmel\Acn²⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0246681

Feature type

allele

Associated gene

Dmel\Acn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Haberman et al., 2010)

Mutagen

ethyl methanesulfonate

(Haberman et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q243term.

(Haberman et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:19,046,293..19,046,293 [+]

Nucleotide change:

C19046293T

Amino acid change:

Q243term | Acn-PA; Q243term | Acn-PB; Q243term | Acn-PC

Reported amino acid change:

Q243term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Haberman et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | somatic clone

(Haberman et al., 2010)

lethal (with Acn¹)

(Haberman et al., 2010)

lethal (with Df(2L)TW130)

(Haberman et al., 2010)

Phenotype Manifest In

embryonic/larval fat body (with Acn¹)

(Haberman et al., 2010)

eye | somatic clone

(Haberman et al., 2010)

eye photoreceptor cell | somatic clone

(Haberman et al., 2010)

ommatidium | somatic clone

(Haberman et al., 2010)

pigment cell | somatic clone

(Haberman et al., 2010)

Detailed Description

Statement

Reference

Flies containing eyes that are homozygous for Acn²⁷ have rust-coloured eyes with no pseudopupil.

Acn¹/Acn²⁷ animals show a distributed lethal phase, with most larvae dying during the third instar, while some die after pupariation.

Hemocytes isolated from Acn¹/Acn²⁷ third instar larvae appear indistinguishable from wild-type hemocytes in response to a treatment which induces apoptosis in wild-type hemocytes; the mutant hemocytes undergo nuclear condensation and chromatin fragmentation.

41% of ommatidia in eyes homozygous for Acn²⁷ have missing or extra photoreceptors or are disorganised. The mutant pigment cells contain high levels of glycogen-like particles compared to wild-type cells.

Autolysosomes are fewer and less abundant, while autophagosomes are larger and more abundant in the fat body of Acn¹/Acn²⁷ third instar larvae than in control larvae after starvation.

(Haberman et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Acn²⁷/Acn[+] is an enhancer of visible | dominant phenotype of N^264-39

(Haberman et al., 2010)

Suppressor of

Statement

Reference

Acn²⁷/Acn[+] is a suppressor of visible | dominant phenotype of Egfr^E1

(Haberman et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Acn²⁷/Acn[+] is an enhancer of wing phenotype of N^264-39

(Haberman et al., 2010)

Suppressor of

Statement

Reference

Acn²⁷/Acn¹ is a suppressor of fat body phenotype of Scer\GAL4^Lsp2.PH, mTor^TED.UAS

(Haberman et al., 2010)

Acn²⁷/Acn¹ is a suppressor of fat body phenotype of Pten^UAS.cGb, Scer\GAL4^Lsp2.PH

(Haberman et al., 2010)

Acn²⁷/Acn[+] is a suppressor of eye phenotype of Egfr^E1

(Haberman et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of Egfr^E1/+ flies is suppressed by Acn²⁷/+.

The severity of the notched wing phenotype seen in N^264-39/+ flies is enhanced by Acn²⁷/+.

Expression of Tor^TED.Scer\UAS under the control of Scer\GAL4^Lsp2.PH cannot induce autolysosome formation in the fat body in a Acn¹/Acn²⁷ background.

Expression of Pten^Scer\UAS.cGb under the control of Scer\GAL4^Lsp2.PH cannot induce autolysosome formation in the fat body in a Acn¹/Acn²⁷ background.

(Haberman et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Acn²⁷/Acn¹ is rescued by Acn^{S641A.S731A.Tag:MYC}

(Nandi et al., 2014)

Acn²⁷/Acn¹ is rescued by Acn^{S641D.S731D.Tag:MYC}

(Nandi et al., 2014)

Acn²⁷/Acn¹ is rescued by Acn^{S641D.S731D.UAS.Tag:MYC}

(Nandi et al., 2014)

Acn²⁷/Acn¹ is rescued by Acn^+t4.2

(Haberman et al., 2010)

Acn²⁷/Acn¹ is rescued by Acn^4.2.Tag:MYC

(Haberman et al., 2010)

Comments

Acn^+t4.2 and Acn^{4.2.T:Hsap\MYC} each restore the viability of Acn¹/Acn²⁷ animals, and rescue the endocytosis and autophagy defects of Acn¹/Acn²⁷ larvae.

(Haberman et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The chromosome probably carries a second-site mutation, because the lethality of Acn¹/Acn²⁷ transheterozygotes, but not of Acn²⁷ homozygotes, can be rescued by Acn^+t4.2 or by Acn^{4.2.T:Hsap\MYC}.

(Haberman et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Acn²⁷

(Nandi et al., 2014)

acn²⁷

(Tyra et al., 2020, Shinoda et al., 2019, Nandi et al., 2017)

dacn²⁷

(Haberman et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (6)

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