FlyBase Allele Report: Scer\GAL4[grh.D4]

General Information

Symbol

Scer\GAL4^grh.D4

Species

S. cerevisiae

Name

FlyBase ID

FBal0335388

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{grh-Gal4.D4}

Also Known As

grh-GAL4, grhNB-Gal4, grhD4Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Chell and Brand, 2010)

Progenitor genotype

Carried in construct

P{grh-Gal4.D4}

Cytology

Description

The D4 grh enhancer (~4kb from the second intron of grh) regulates expression of a GAL4 driver.

(Chell and Brand, 2010)

Allele components

Component

Use(s)

Regulatory region(s)

grh

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

thoracic neuroblast | subset

Comment: 0-1 hours after hatching time

(Chell and Brand, 2010)

second instar larval stage

thoracic neuroblast | subset

Comment: 24 hours after hatching time

(Chell and Brand, 2010)

early third instar larval stage

thoracic neuroblast | subset

Comment: 48 hours after hatching time

(Chell and Brand, 2010)

Additional Information

Statement

Reference

ScerGAL4^grh.D4 is expressed in approximately 1/3rd of thoracic neuroblasts during larval development.

(Chell and Brand, 2010)

Marker for

Reflects expression of

grh

(Chell and Brand, 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | larval stage, with Chc^JF02681

(Wingen et al., 2017)

decreased body size | larval stage, with grh^JF02305

(Wingen et al., 2017)

decreased body size | larval stage, with Rab5^GD10492

(Wingen et al., 2017)

decreased body size | larval stage, with Rab5^S43N.UAS

(Wingen et al., 2017)

decreased body size | larval stage, with Rab11^JF02812

(Wingen et al., 2017)

decreased body size | larval stage, with Rab11^{S25N.UASp.YFP}

(Wingen et al., 2017)

decreased body size | larval stage, with Rab32^KK107695

(Wingen et al., 2017)

decreased body size | larval stage, with Rab32^{T33N.UASp.YFP}

(Wingen et al., 2017)

decreased body size | larval stage, with Sec5^JF02676

(Wingen et al., 2017)

decreased body size | larval stage, with Sec6^JF02623

(Wingen et al., 2017)

decreased body size | larval stage, with Sec10^JF02633

(Wingen et al., 2017)

decreased body size | larval stage, with Sec15^JF02649

(Wingen et al., 2017)

decreased body size | larval stage, with shi^K44A.UAS

(Wingen et al., 2017)

decreased occurrence of cell division | larval stage, with arm^GD1372

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with arm^HMS01414

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with arm^KK102545

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with dally^RNAi.UAS.cUa

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with dsh^JF01254

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with fz2^JF01378

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with fz^JF01258

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with sgg^S9A.UAS

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with simba1^KK108629

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with simba2^KK111047

(Lin et al., 2025)

decreased occurrence of cell division | larval stage, with wg^GD5007

(Lin et al., 2025)

increased occurrence of cell division | larval stage, with simba1^{UAS.ORF.GW.Tag:HA}

(Lin et al., 2025)

increased occurrence of cell division | larval stage, with simba1^UAS.ORF-CC

(Lin et al., 2025)

lethal, with Chc^JF02681

(Wingen et al., 2017)

lethal, with dsb^GD2254, dsb^KK107147

(Wingen et al., 2017)

lethal, with Rab5^GD10492

(Wingen et al., 2017)

lethal, with Rab5^KK102895

(Wingen et al., 2017)

lethal, with Rab5^S43N.UAS

(Wingen et al., 2017)

lethal, with Rab11^JF02812

(Wingen et al., 2017)

lethal, with Rab11^{S25N.UASp.YFP}

(Wingen et al., 2017)

lethal, with Sec5^JF02676

(Wingen et al., 2017)

lethal, with Sec6^JF02623

(Wingen et al., 2017)

lethal, with Sec10^JF02633

(Wingen et al., 2017)

lethal, with Sec15^JF02649

(Wingen et al., 2017)

lethal, with shi^K44A.UAS

(Wingen et al., 2017)

partially lethal, with dsb^GD2254

(Wingen et al., 2017)

partially lethal, with dsb^KK107147

(Wingen et al., 2017)

partially lethal, with grh^GL01069

(Wingen et al., 2017)

partially lethal, with grh^JF02305

(Wingen et al., 2017)

partially lethal, with grh^KK109135

(Wingen et al., 2017)

some die during larval stage, with dsb^GD2254, dsb^KK107147

(Wingen et al., 2017)

visible | larval stage, with Chc^JF02681

(Wingen et al., 2017)

visible | larval stage, with grh^GL01069

(Wingen et al., 2017)

visible | larval stage, with grh^JF02305

(Wingen et al., 2017)

visible | larval stage, with grh^KK109135

(Wingen et al., 2017)

visible | larval stage, with Rab5^GD10492

(Wingen et al., 2017)

visible | larval stage, with Rab5^KK102895

(Wingen et al., 2017)

visible | larval stage, with Rab5^S43N.UAS

(Wingen et al., 2017)

visible | larval stage, with Rab11^JF02812

(Wingen et al., 2017)

visible | larval stage, with Rab11^{S25N.UASp.YFP}

(Wingen et al., 2017)

visible | larval stage, with Sec5^JF02676

(Wingen et al., 2017)

visible | larval stage, with Sec6^JF02623

(Wingen et al., 2017)

visible | larval stage, with Sec10^JF02633

(Wingen et al., 2017)

visible | larval stage, with Sec15^JF02649

(Wingen et al., 2017)

visible | larval stage, with shi^K44A.UAS

(Wingen et al., 2017)

Phenotype Manifest In

embryonic/larval posterior spiracle | larval stage, with Chc^JF02681

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with dsb^GD2254, dsb^KK107147

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with grh^GL01069

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with grh^JF02305

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with grh^KK109135

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Rab5^GD10492

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Rab5^KK102895

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Rab5^S43N.UAS

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Rab11^JF02812

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Rab11^{S25N.UASp.YFP}

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Sec5^JF02676

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Sec6^JF02623

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Sec10^JF02633

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with Sec15^JF02649

(Wingen et al., 2017)

embryonic/larval posterior spiracle | larval stage, with shi^K44A.UAS

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Chc^JF02681

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with grh^GL01069

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with grh^JF02305

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with grh^KK109135

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Rab5^GD10492

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Rab5^KK102895

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Rab5^S43N.UAS

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Rab11^JF02812

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Rab11^{S25N.UASp.YFP}

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Sec5^JF02676

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Sec6^JF02623

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Sec10^JF02633

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Sec15^JF02649

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | first instar larval stage, with shi^K44A.UAS

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | larval stage, with dsb^GD2254

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | larval stage, with dsb^GD2254, dsb^KK107147

(Wingen et al., 2017)

embryonic/larval tracheal dorsal trunk | larval stage, with dsb^KK107147

(Wingen et al., 2017)

larval neuroblast, with arm^GD1372

(Lin et al., 2025)

larval neuroblast, with arm^HMS01414

(Lin et al., 2025)

larval neuroblast, with arm^KK102545

(Lin et al., 2025)

larval neuroblast, with dally^RNAi.UAS.cUa

(Lin et al., 2025)

larval neuroblast, with dsh^JF01254

(Lin et al., 2025)

larval neuroblast, with fz2^JF01378

(Lin et al., 2025)

larval neuroblast, with fz^JF01258

(Lin et al., 2025)

larval neuroblast, with sgg^S9A.UAS

(Lin et al., 2025)

larval neuroblast, with simba1^KK108629

(Lin et al., 2025)

larval neuroblast, with simba1^{UAS.ORF.GW.Tag:HA}

(Lin et al., 2025)

larval neuroblast, with simba1^UAS.ORF-CC

(Lin et al., 2025)

larval neuroblast, with simba2^KK111047

(Lin et al., 2025)

larval neuroblast, with wg^GD5007

(Lin et al., 2025)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^grh.D4/wg^UAS.GFP is a suppressor | partially of decreased occurrence of cell division | larval stage phenotype of simba2^H3

(Lin et al., 2025)

Scer\GAL4^grh.D4/wg^UAS.GFP is a suppressor | partially of decreased occurrence of cell division | larval stage phenotype of simba1^D5

(Lin et al., 2025)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^grh.D4/wg^UAS.GFP is a suppressor | partially of larval neuroblast phenotype of simba2^H3

(Lin et al., 2025)

Scer\GAL4^grh.D4/wg^UAS.GFP is a suppressor | partially of larval neuroblast phenotype of simba1^D5

(Lin et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

simba1^UAS.ORF-CC/Scer\GAL4^grh.D4 partially rescues simba1^D5

(Lin et al., 2025)

simba2^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^grh.D4 partially rescues simba2^H3

(Lin et al., 2025)

Scer\GAL4^grh.D4/dsb^UAS.D partially rescues dsb^ko

(Wingen et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^grh.D4

Name Synonyms

Secondary FlyBase IDs

References (17)

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