FlyBase Gene Report: Dmel\ND-PDSW

General Information

Symbol

Dmel\ND-PDSW

Species

D. melanogaster

Name

NADH dehydrogenase (ubiquinone) PDSW subunit

Annotation Symbol

CG8844

Feature Type

protein_coding_gene

FlyBase ID

FBgn0021967

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Pdsw, dNDUFB10, l(2)k10101

Key Links

Genomic Location

Cytogenetic map

23F3-23F3

Sequence location

2L:3,466,157..3,467,334 [+]

Recombination map

2-10

RefSeq locus

NT_033779 REGION:3466157..3467334

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mitochondrial electron transport, NADH to ubiquinone

inferred by curator from GO:0045271

(Agip et al., 2023)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Alonso et al., 2005)

part_of respiratory chain complex I

inferred from direct assay

(Agip et al., 2023)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrial inner membrane

inferred by curator from GO:0045271

(Agip et al., 2023)

part_of respiratory chain complex I

inferred from sequence or structural similarity with UniProtKB:O96000

(Gene Ontology Curators, 2002-)

non-traceable author statement

(Rhooms et al., 2020, Porcelli et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN001405974

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX I - ACCESSORY SUBUNITS

MITOCHONDRIAL COMPLEX I - TESTIS-SPECIFIC VARIANT

MITOCHONDRIAL COMPLEX I

Pathway (FlyBase)

OXIDATIVE PHOSPHORYLATION

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX I - ACCESSORY SUBUNITS -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. 'Accessory' or 'supernumerary' subunits are all encoded by nuclear DNA and are not directly involved in catalysis. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I - TESTIS-SPECIFIC VARIANT -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)

Pathway (FlyBase)

OXIDATIVE PHOSPHORYLATION -: Oxidative phosphorylation (OXPHOS) is the coordinated transfer of electrons and protons leading to the production of ATP. The OXPHOS system is composed of five mitochondrial inner membrane complexes, Complexes I-IV comprise the electron respiratory chain and Complex V the ATP synthase. Complexes I and II oxidize the electron donors NADH and FADH2, respectively, derived from the tricarboxylic acid (TCA) cycle, and the electrons are transferred to the electron carrier ubiquinone which shuttles them to Complex III. Cytochrome c transfers electrons between Complex III and the terminal complex in the respiratory chain, Complex IV. Complex IV catalyzes electron transfer from reduced cytochrome c to oxygen. Complex I, II and IV couple electron transfer to proton transport across the inner mitochondrial membrane into the inner membrane space. This generates a proton electrochemical gradient which is used to drive the synthesis of ATP from ADP and inorganic phosphate by ATP synthase (Complex V). (Adapted from PMID:23356252 and PMID:11331900.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ND-PDSW for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VQR2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ND-PDSW-RA

FBtr0077520

NM_143715

651

159

ND-PDSW-RB

FBtr0077521

NM_164537

738

159

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ND-PDSW-PA

FBpp0077209

18.9

159

7.03

FBpp0077210

18.9

159

7.03

Q9VQR2

NP_722908

AAN10369

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

159 aa isoforms: ND-PDSW-PA, ND-PDSW-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VQR2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ND-PDSW using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.36

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic large intestine

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Alonso et al., 2005)

part_of respiratory chain complex I

inferred from direct assay

(Agip et al., 2023)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ND-PDSW in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ND-PDSW

View exonic expression by tissue for Dmel\ND-PDSW

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0021967

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44228/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0021967

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0021967

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0021967

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8844

Flygut - An atlas of the Drosophila adult midgut

FBgn0021967

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ND-PDSW

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ND-PDSW

Transgenic constructs containing regulatory region of ND-PDSW

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene not disrupted in

Df(2L)JS17

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)S2590

(BDGP Project Members, 1994-1999)

In(2LR)DTD16^LDTD42^R

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene disrupted in

Df(2L)BSC31

(Deal and Cook, 2003.4.2)

Df(2L)Exel7016

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

ND-PDSW^GD12532

lethal | recessive

ND-PDSW^k10101

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

ND-PDSW^GD12532

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ND-PDSW^GD12532

viable, with Scer\GAL4^pnr-MD237

ND-PDSW^GD12532

viable, with Scer\GAL4^tin.CΔ4

ND-PDSW^GD12532

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

ND-PDSW^GD12532

decreased occurrence of cell division | larval stage

ND-PDSW^k10101

increased cell number | third instar larval stage, with Scer\GAL4^Antp-10

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

ND-PDSW^GD12532

visible, with Scer\GAL4^pnr-MD237

ND-PDSW^GD12532

visible | recessive | somatic clone

ND-PDSW^k10101

Phenotype manifest in

Allele

eye | somatic clone

ND-PDSW^k10101

eye disc | somatic clone

ND-PDSW^k10101

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

ND-PDSW^GD12532

lamellocyte | third instar larval stage, with Scer\GAL4^Antp-10

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

ND-PDSW^GD12532

lens | somatic clone

ND-PDSW^k10101

trichogen cell, with Scer\GAL4^pnr-MD237

ND-PDSW^GD12532

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\NDUFB10

14 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Ndufb10

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Ndufb10

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\ndufb10

7 of 13

Yes

Xtro\elp6

5 of 13

Xtro\osbpl1a

1 of 13

Danio rerio (Zebrafish) (1)

Drer\ndufb10

14 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\F59C6.5

12 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP007780

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\AT1G49140

2 of 13

Yes

Atha\AT3G18410

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ND-PDSW. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, intercellular interactions, RAS-mito

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

NDUFB10; NADH:ubiquinone oxidoreductase subunit B10

model of nuclear type mitochondrial complex I deficiency 35

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

ND-PDSW^k10101

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

ND-PDSW^JF03271

ameliorates cancer

modeled by Hipk^UAS.Tag:HA

(Wong et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NDUFB10; NADH:ubiquinone oxidoreductase subunit B10

14 of 14

603843

MITOCHONDRIAL COMPLEX I DEFICIENCY, NUCLEAR TYPE 35; MC1DN35

nuclear type mitochondrial complex I deficiency 35

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ND-PDSW - Actn

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Adh

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Argk1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Gdh

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Gpdh1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Mf

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Mlc1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Mlc2

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Mpcp1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - ND-13A

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-13B

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-15

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-19

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-20

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-23

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-24

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-30

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-39

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-42

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-49

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - ND-51

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-75

cosedimentation, electron microscopy, molecular weight estimation by staining, experimental knowledge based

(Padavannil et al., 2023, Guruharsha et al., 2011)

ND-PDSW - ND-ACP

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-AGGG

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-ASHI

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B12

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B14

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B14.5A

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B14.5B

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B14.7

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B15

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B16.6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B17

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B17.2

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B18

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-B22

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-MNLL

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-MWFE

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - ND-SGDH

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - NP15.6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - NdufA3

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - Ogdh1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Scp1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - Tm2

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - mAcon1

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - mt:ND1

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - mt:ND2

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - mt:ND3

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - mt:ND4L

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - mt:ND5

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - mt:ND6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-PDSW - up

experimental knowledge based

(Guruharsha et al., 2011)

ND-PDSW - wupA

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

peb

suppressible

ND-PDSW

(Wilk et al., 2004)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0021967

MIST (protein-protein) - An integrated Molecular Interaction Database

44228

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

OXIDATIVE PHOSPHORYLATION

External Links

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-10

Cytogenetic map

23F3-23F3

Sequence location

2L:3,466,157..3,467,334 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23F3-23F3

Limits computationally determined from genome sequence between P{lacW}Mad^k00237 and P{lacW}Pdsw^k10101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

23F3-23F4

(determined by in situ hybridisation)

(Spradling et al., 1999)

23F3-23F4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-10

(FlyBase, 2016)

2-8.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29592

y¹ v¹; P{TRiP.JF03271}attP2

10965

y¹ w^67c23; P{lacW}ND-PDSW^k10101/CyO

6506

y¹ w^*; Df(2L)drm-P1, P{lacW}ND-PDSW^k10101/SM6b

6507

y¹ w^*; Df(2L)drm-P2, P{lacW}ND-PDSW^k10101/SM6b

Kyoto

108814

y¹ w^*; Df(2L)drm-P1, P{lacW}ND-PDSW^k10101/SM6b

108815

y¹ w^*; Df(2L)drm-P2, P{lacW}ND-PDSW^k10101/SM6b

111278

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}ND-PDSW^k10101 P{neoFRT}40A/CyO y⁺

NIG-Fly

8844R-2

P{NIG.8844R}2

2LG-1156

y² VhaAC39-1^cho-1 v¹; P{U6-ND-PDSW.NIG.sgRNA}attP40/CyO

VDRC

v106095

P{KK108420}VIE-260B

v35437

w¹¹¹⁸; P{GD12532}v35437

v318628

PBac{fTRG00635.sfGFP-TVPTBF}VK00033

Genomic Clones (8)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (36)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

AF171793

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0021967

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0021967

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Hossain et al., 2022)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: Pdsw CG8844

(Misra, 2000.8.9)

Source for merge of: Pdsw anon- EST:Posey91

(Bayraktaroglu, 2000.11.2)

Source for merge of: Pdsw BcDNA:RE65754

(FlyBase, 1996-)

Additional comments

Source for merge of Pdsw BcDNA:RE65754 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: ND-PDSW Pdsw

(Porcelli and Marygold, 2014.9.16)

Nomenclature comments

Renamed from 'Pdsw' to 'ND-PDSW' to match the format of other genes encoding NADH dehydrogenase (ubiquinone) subunits.

(Porcelli and Marygold, 2014.9.16)

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

BcDNA:RE65754

CG8844

(Curcio et al., 2024, Murari et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Murari et al., 2020, Rhooms et al., 2020, Çiçek et al., 2016, Hosono et al., 2015, Owusu-Ansah et al., 2013, Jaspers et al., 2012, Cammarato et al., 2011, Thornton et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Porcelli et al., 2007, Caggese et al., 2006)

ND-PDSW

(Curcio et al., 2024, Salminen et al., 2024, Ren et al., 2023, Groen et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Sen and Cox, 2022, Sinenko et al., 2021, Myat et al., 2019, Gene Disruption Project members, 2018-, Porcelli and Marygold, 2014.9.16)

NDUFB10

(Agip et al., 2023)

NIDM

(Caggese et al., 2006)

Ndufb10

(Meyer et al., 2024)

PDSW

(Owusu-Ansah et al., 2013, Sinenko et al., 2011, Sinenko et al., 2011)

Pdsw

(Mo et al., 2023, Sarov et al., 2016, Japanese National Institute of Genetics, 2012.5.21, Malone et al., 2012, Rohn et al., 2011, Wasbrough et al., 2010, Owusu-Ansah et al., 2008, Kaltenbach et al., 2007, Caggese et al., 2006, Alonso et al., 2005, Mandal et al., 2005, Tripoli et al., 2005)

Q9VQR2

(Ebanks et al., 2021)

anon-EST:Posey91

dNDUFB10

(Murari et al., 2023, Murari et al., 2020, Rhooms et al., 2020)

l(2)k10101

(BDGP Project Members, 1994-1999)

mito

(Enomoto and Igaki, 2022)

pdsw

(Wong et al., 2020, Toshniwal et al., 2019, Wang et al., 2012, Liao et al., 2006)

Name Synonyms

NADH dehydrogenase (ubiquinone) PDSW subunit

(Porcelli and Marygold, 2014.9.16)

NADH ubiquinone oxidoreductase PDSW

(Porcelli et al., 2007)

NADH-ubiquinone oxidoreductase PDSW subunit

(Caggese et al., 2006)

NADH:ubiquinone oxidoreductase PDSW subunit

(Tripoli et al., 2005)

Pdsw

(Cammarato et al., 2011)

Secondary FlyBase IDs

FBgn0027663
FBgn0031539
FBgn0062981

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44228

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VQR2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VQR2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VQR2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0021967

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44228/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0021967

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0021967

FlyMine - An integrated database for Drosophila genomics

FBgn0021967

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8844

MARRVEL_MODEL - MARRVEL (model organism gene)

44228

PDB - An information portal to biological macromolecular structures

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0021967

DroID - A comprehensive database of gene and protein interactions.

FBgn0021967

DRSC - Results frm RNAi screens

FBgn0021967

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0021967

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0021967

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8844

Flygut - An atlas of the Drosophila adult midgut

FBgn0021967

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0021967

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006357

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

44228

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (90)

The following external sites may use different assemblies or annotations than FlyBase.

3.36

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