FlyBase Gene Report: Dmel\fws

General Information

Symbol

Dmel\fws

Species

D. melanogaster

Name

four way stop

Annotation Symbol

CG6549

Feature Type

protein_coding_gene

FlyBase ID

FBgn0024689

Gene Model Status

Stock Availability

7 publicly available

Gene Summary

four way stop (fws) encodes a protein localised to the Golgi apparatus and involved in several aspects of spermatogenesis. Loss of function of fws causes failure of cleavage furrow ingression in dividing spermatocytes, as well as failure of cell elongation and disruption of the Golgi-based acroblast during spermatid differentiation [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Cog5, dCOG5

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:17,478,442..17,481,029 [-]

Recombination map

2-53

RefSeq locus

NT_033779 REGION:17478442..17481029

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2016-03-17 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in acrosome assembly

inferred from mutant phenotype

(Fári et al., 2016)

involved_in actomyosin contractile ring contraction

inferred from mutant phenotype

(Giansanti et al., 2004)

involved_in male meiosis cytokinesis

inferred from mutant phenotype

(assigned by UniProt )

(Farkas et al., 2003)

involved_in meiosis I cytokinesis

inferred from mutant phenotype

(Giansanti et al., 2004)

involved_in meiosis II cytokinesis

inferred from mutant phenotype

(Giansanti et al., 2004)

involved_in meiotic spindle organization

inferred from mutant phenotype

(Giansanti et al., 2004)

involved_in spermatid nucleus elongation

inferred from mutant phenotype

(assigned by UniProt )

(Farkas et al., 2003)

inferred from mutant phenotype

(Fári et al., 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intra-Golgi vesicle-mediated transport

inferred from sequence or structural similarity with SGD:S000004996

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000325596

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in endoplasmic reticulum exit site

inferred from direct assay

(Herpers and Rabouille, 2004)

located_in Golgi apparatus

inferred from direct assay

(assigned by UniProt )

(Farkas et al., 2003)

part_of Golgi transport complex

inferred from direct assay

(Gillingham et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of Golgi transport complex

inferred from biological aspect of ancestor with PANTHER:PTN000325596

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CONSERVED OLIGOMERIC GOLGI COMPLEX

Protein Family (UniProt)

Belongs to the COG5 family. (Q9VJD3)

Protein Signatures (InterPro)

Conserved oligomeric Golgi complex subunit 5

Summaries

Gene Snapshot

four way stop (fws) encodes a protein localised to the Golgi apparatus and involved in several aspects of spermatogenesis. Loss of function of fws causes failure of cleavage furrow ingression in dividing spermatocytes, as well as failure of cell elongation and disruption of the Golgi-based acroblast during spermatid differentiation [Date last reviewed: 2018-09-20]

Gene Group (FlyBase)

CONSERVED OLIGOMERIC GOLGI COMPLEX -: The conserved oligomeric Golgi (COG) complex is a multi-subunit tethering complex of the CATCHR family that functions as a vesicular tether during retrograde intra-Golgi trafficking. (Adapted from PMID:23839779).

Protein Function (UniProtKB)

Required for normal Golgi function and necessary during spermatogenesis. Required for cleavage furrow ingression during cytokinesis in dividing spermatocytes and for the extensive polarized cell growth that accompanies spermatid elongation.

(UniProt, Q9VJD3)

Gene Model and Products

Number of Transcripts

5

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\fws for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.45

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.57

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0081000

2477

751

FBtr0080999

2428

751

FBtr0305685

2424

751

FBtr0346626

2447

751

FBtr0346627

2398

751

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0080553

85.0

751

6.69

FBpp0080552

85.0

751

6.69

FBpp0296965

85.0

751

6.69

FBpp0312206

85.0

751

6.69

FBpp0312207

85.0

751

6.69

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

751 aa isoforms: fws-PA, fws-PC, fws-PD, fws-PH, fws-PI

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.

(UniProt, Q9VJD3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Conserved oligomeric Golgi complex subunit 5

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fws using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fws_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval salivary gland

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endoplasmic reticulum exit site

inferred from direct assay

(Herpers and Rabouille, 2004)

located_in Golgi apparatus

inferred from direct assay

(assigned by UniProt )

(Farkas et al., 2003)

part_of Golgi transport complex

inferred from direct assay

(Gillingham et al., 2014)

Expression Deduced from Reporters

Reporter: P{fws::GFP}

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & third instar larval stage

(Polevoy et al., 2009)

adult stage | male & spermatogenesis

• Golgi apparatus

(Belloni et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\fws in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\fws

View exonic expression by tissue for Dmel\fws

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024689

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024689

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6549-RC

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6549

Flygut - An atlas of the Drosophila adult midgut

FBgn0024689

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\fws

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of fws

Transgenic constructs containing regulatory region of fws

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

(Ryder, 2004.4.26)

Df(2L)qua¹³⁷⁴

(Giansanti et al., 2004)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fws^GD11957

viable, with Scer\GAL4^pnr-MD237

fws^GD11957

viable, with Scer\GAL4^tin.CΔ4

fws^GD11957

Sterility

Allele

fws^KG02853

Other Phenotypes

Allele

abnormal cytokinesis | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

abnormal cytokinesis | male | recessive

abnormal meiotic cell cycle | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

abnormal meiotic cell cycle | male | recessive

abnormal mitotic cell cycle | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

abnormal mitotic cell cycle | male | recessive

Phenotype manifest in

Allele

fws^KG02853

acrosomal vesicle

fws^KG02853

contractile ring & meiotic telophase I | male

fws^Z0161

contractile ring & meiotic telophase I | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

contractile ring & meiotic telophase II | male

fws^Z0161

contractile ring & meiotic telophase II | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

investment cone

fws^KG02853

Nebenkern (with Df(2L)qua¹³⁷⁴)

fws^Z0161

spermatid & nucleus | supernumerary

spermatid & nucleus | supernumerary (with Df(2L)qua¹³⁷⁴)

fws^Z0161

spindle & meiotic telophase I | male

fws^Z0161

spindle & meiotic telophase I | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

spindle & meiotic telophase II | male

fws^Z0161

spindle & meiotic telophase II | male (with Df(2L)qua¹³⁷⁴)

fws^Z0161

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

1

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

Rattus norvegicus (Norway rat) (2)

4 of 13

Yes

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (1)

2 of 12

Yes

Yes

Danio rerio (Zebrafish) (1)

13 of 15

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

11 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (1)

8 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

2 of 15

Yes

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

1 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903YS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00001121

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915032O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY003080

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07602

Mayetiola destructor

Hessian fly

Mdes\Mdes000254

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005180

Anopheles darlingi

American malaria mosquito

Adar\ADAC009157

Anopheles gambiae

Malaria mosquito

Agam\AGAP005853

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014696

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05L0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006777

Bombyx mori

Silkmoth

Bmor\BGIBMGA007170

Danaus plexippus

Monarch butterfly

Dple\DPOGS203721-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS215073-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL010544

Heliconius melpomene

Postman butterfly

Hmel\HMEL008534

Apis florea

Little honeybee

Aflo\3963632852

Apis florea

Little honeybee

Aflo\3476052788

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001b74

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0023b4

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013117

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM380220

Pediculus humanus

Human body louse

Phum\PHUM418010

Rhodnius prolixus

Kissing bug

Rpro\RPRC014369

Rhodnius prolixus

Kissing bug

Rpro\RPRC009002

Cimex lectularius

Bed bug

Clec\CLEC007073

Cimex lectularius

Bed bug

Clec\CLEC009192

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005725

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003998

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14918

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07902

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02RS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR000305

Strigamia maritima

European centipede

Smar\SMAR000990

Ixodes scapularis

Black-legged tick

Isca\ISCW015962

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65540.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57375.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g14040

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g10250

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_70302

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_299680

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230543

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G048P )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_024732gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_026186gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdc2l1_1

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk10_1

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008691gn

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000000488

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000010856

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000020691

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

COG5; component of oligomeric golgi complex 5

model of congenital disorder of glycosylation type IIi

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

COG5; component of oligomeric golgi complex 5

13 of 15

CONGENITAL DISORDER OF GLYCOSYLATION, TYPE IIi; CDG2I

congenital disorder of glycosylation type IIi

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

two hybrid, pull down, western blot

(Frappaolo et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.

(UniProt, Q9VJD3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61050

DroID - A comprehensive database of gene and protein interactions.

FBgn0024689

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35054

MIST (protein-protein) - An integrated Molecular Interaction Database

fws

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-53

Cytogenetic map

Sequence location

2L:17,478,442..17,481,029 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36C10-36C10

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

36C-36C

(Giansanti et al., 2004)

Location based on deficiency mapping.

(Giansanti et al., 2004)

Experimentally Determined Recombination Data

Location

2-53

(FlyBase, 2016)

2-52.7

(Comeron et al., 2012)

2-

(Giansanti et al., 2004, Brill et al., 1998.3.16)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

13496

y¹; P{SUPor-P}fws^KG02853

66717

fws^Z0161 cn¹ bw¹/CyO

79758

y¹ sc^* v¹ sev²¹; P{TKO.GS01785}attP40

79846

y¹ sc^* v¹ sev²¹; P{TOE.GS01497}attP40

56882

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04103}attP40

v110378

P{KK101056}VIE-260B

v27661

w¹¹¹⁸ P{GD11957}v27661

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (230)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

AF171831

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024689

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024689

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35054

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG6549 anon- EST:Posey162

(Bayraktaroglu, 2000.11.2)

Source for merge of: fws CG6549

(Farkas, 2001.3.12)

Source for merge of: fws anon- EST:Posey186

(Ashburner, 2004.6.30)

Additional comments

Other Comments

Mutants are viable and male sterile.

(Farkas, 2001.3.12)

The phenotype of mutants is a failure in meiotic cytokinesis ("four wheel drive" phenotype), as well as a failure in proper polarization and bundling of the cysts of elongating spermatids. The end result is an accumulation of ovoid cysts containing multinucleate spermatids. The flies are male sterile.

(Brill et al., 1998.3.16)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35054

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VJD3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NCV1

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024689

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0024689

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fws_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6549

Flygut - An atlas of the Drosophila adult midgut

FBgn0024689

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35054

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013924

InterPro - A database of protein families, domains and functional sites

Conserved oligomeric Golgi complex subunit 5

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6549

modMine - A data warehouse for the modENCODE project

FBgn0024689

Linkouts

BioGRID - A database of protein and genetic interactions.

61050

DroID - A comprehensive database of gene and protein interactions.

FBgn0024689

DRSC - Results frm RNAi screens

FBgn0024689

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024689

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6549-RC

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0024689

FlyMine - An integrated database for Drosophila genomics

FBgn0024689

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35054

MIST (protein-protein) - An integrated Molecular Interaction Database

fws

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG6549

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ashburner, 2004.6.30, Farkas et al., 2003)

COG5

(Hartwig et al., 2021, Ungar et al., 2006)

Cog5

(Frappaolo et al., 2017, Sechi et al., 2017, Belloni et al., 2012, Fabian and Brill, 2012, Giansanti et al., 2012, Giansanti et al., 2006)

FWS

(Fan et al., 2011)

Fws

(Frappaolo et al., 2017, Fabian and Brill, 2012, Zhou et al., 2011, Polevoy et al., 2009, Robinett et al., 2009)

anon-EST:Posey162

anon-EST:Posey186

dCOG5

(Nicolas et al., 2009, Lee and Cooley, 2007, Herpers and Rabouille, 2004)

fws

(Transgenic RNAi Project members, 2017-, Fári et al., 2016, Gillingham et al., 2014, Belloni et al., 2012, Giansanti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lesch and Page, 2012, Koch et al., 2009, Smith and Lupashin, 2008, Lee and Cooley, 2007, Giansanti et al., 2006, Morris et al., 2006, Stanyon et al., 2004, Farkas et al., 2003)

Name Synonyms

Drosophila Cog5

(Farkas et al., 2003)

Four Way Stop

(Zhou et al., 2011)

Four-way stop

(Robinett et al., 2009)

four way stop

(Lesch and Page, 2012, Giansanti et al., 2006, Farkas et al., 2003, Brill et al., 1998.3.16)

Secondary FlyBase IDs

FBgn0027736
FBgn0027744
FBgn0032641

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (61)