FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\temp
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General Information
Symbol
Dmel\temp
Species
D. melanogaster
Name
tempura
Annotation Symbol
CG3073
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027296
Gene Model Status
Current
Stock Availability
8 publicly available
Enzyme Name (EC)
protein geranylgeranyltransferase type II (2.5.1.60)
Gene Summary
tempura (temp) encodes a protein-prenyltransferase subunit that regulates Notch signaling. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
2F2-2F2
Sequence location
Recombination map
1-0.9
RefSeq locus
NC_004354 REGION:2260207..2262515
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to Rab geranylgeranyltransferase activity
inferred from direct assay
(Charng et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables protein prenyltransferase activity
inferred from electronic annotation with InterPro:IPR002088
(InterPro Project Members, 2004-)
contributes_to Rab geranylgeranyltransferase activity
inferred from biological aspect of ancestor with PANTHER:PTN008512764
(GO Reference Genome Project, 2011-)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in sensory organ development
inferred from mutant phenotype
(Charng et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Charng et al., 2014)
part_of Rab-protein geranylgeranyltransferase complex
inferred from direct assay
(Charng et al., 2014)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000122800
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
Rab geranylgeranyltransferase activity
geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L- cysteinyl-[protein] + diphosphate (2.5.1.60)
Summaries
Gene Snapshot
tempura (temp) encodes a protein-prenyltransferase subunit that regulates Notch signaling. [Date last reviewed: 2019-09-19]
Gene Group (FlyBase)
PROTEIN PRENYLTRANSFERASES -
Protein prenyltransferases are heterodimeric enzymes that catalyze the transfer of the carbon moiety of C[[15]] farnesyl pyrophosphate (FPP) or C[[20]] geranylgeranyl pyrophosphate (GGPP) to a conserved cysteine residue in a CaaX motif of protein and peptide substrates. This postranslational modification is essential for a number of proteins including Ras, Rab, nuclear lamins, trimeric G-protein gamma subunits, protein kinases, and small Ras-related GTP-binding proteins. The addition of a farnesyl group to these proteins, for example, is required to anchor them to the cell membrane, a step required for them to function. (Adapted from PMID:12135472, PMID:12702202 and PMID:26216239.)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\temp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W501)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
temp-RA
FBtr0070414
NM_130636
2163
398
temp-RB
FBtr0345316
NM_001297889
1791
398
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
temp-PA
FBpp0070398
45.8
398
7.98
Q9W501
NP_569992
AAF45764
temp-PB
FBpp0311476
45.8
398
7.98
Q9W501
NP_001284818
AHN59289
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

398 aa isoforms: temp-PA, temp-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\temp using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.85

Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Charng et al., 2014)
part_of Rab-protein geranylgeranyltransferase complex
inferred from direct assay
(Charng et al., 2014)
Expression Deduced from Reporters
Reporter: PBac{HA-gtemp}
Stage
Tissue/Position (including subcellular localization)
Reference
prepupal stage P4
external sensory organ precursor cell
(Charng et al., 2014)
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\temp in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of temp
Transgenic constructs containing regulatory region of temp
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
Hsap\PTAR1
11 of 14
Yes
Yes
 
1  
Hsap\FNTA
3 of 14
No
No
1  
Hsap\RABGGTA
3 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Ptar1
11 of 14
Yes
Yes
Rnor\Fnta
3 of 14
No
No
Rnor\Rabggta
3 of 14
No
No
Mus musculus (laboratory mouse) (3)
Mmus\Ptar1
11 of 14
Yes
Yes
Mmus\Rabggta
3 of 14
No
No
Mmus\Fnta
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\ptar1
6 of 13
Yes
Yes
Xtro\fnta
2 of 13
No
No
Xtro\rabggta
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\ptar1
11 of 14
Yes
Yes
Drer\rabggta
3 of 14
No
No
Drer\fnta
2 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\fnta-1
3 of 14
Yes
No
Cele\M57.2
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (3)
Agam\AgaP_AGAP002731
12 of 12
Yes
Yes
Agam\AgaP_AGAP009724
3 of 12
No
No
Agam\AgaP_AGAP011767
3 of 12
No
No
Arabidopsis thaliana (thale-cress) (4)
Atha\AT1G10095
9 of 13
Yes
Yes
Atha\FTA
3 of 13
No
No
Atha\RGTA1
3 of 13
No
No
Atha\RGTA2
3 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\BET4
2 of 13
Yes
No
Scer\ECM9
2 of 13
Yes
Yes
Scer\RAM2
2 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\cwp1
2 of 12
Yes
No
Spom\SPCC1620.05
2 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:temp. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
Fnta
5 of 13
RabGGTa
5 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PTAR1; protein prenyltransferase alpha subunit repeat containing 1
    11 of 14
    621024
         
        FNTA; farnesyltransferase, CAAX box, subunit alpha
        3 of 14
        134635
             
            RABGGTA; Rab geranylgeranyltransferase subunit alpha
            3 of 14
            601905
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Dmel gene
                Ortholog showing functional complementation
                Supporting References
                temp
                Hsap\PTAR1
                (Charng et al., 2014)
                Interactions
                Summary of Physical Interactions
                Summary of Genetic Interactions
                Interaction Browsers
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                External Data
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                X
                Recombination map
                1-0.9
                Cytogenetic map
                2F2-2F2
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                2F2-2F2
                Limits computationally determined from genome sequence between P{EP}EP1460&P{EP}CG2924EP1596 and P{EP}EP1606
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                2F-2F
                (determined by in situ hybridisation)
                (Peter et al., 2002, Schäfer et al., 1999.6.15)
                Experimentally Determined Recombination Data
                Location

                1-0.9

                (FlyBase, 2016)

                1-0.2

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (8)
                Genomic Clones (17)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (43)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      temp functions as the α subunit of a variant Rab geranylgeranyltransferase (RabGGT) complex which modulates Notch signaling via Rab1 and Rab11.

                      (Charng et al., 2014)

                      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                      (Kiger et al., 2003)
                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: l(1)G0144 CG3073

                      (Peter et al., 2002)
                      Additional comments
                      Nomenclature History
                      Source for database identify of

                      Source for identity of: temp l(1)G0144

                      (Charng et al., 2014)
                      Nomenclature comments
                      Etymology

                      The gene is named "tempura", based on a Japanese deep-fried dish, based on its role in adding lipids to its substrates.

                      (Charng et al., 2014)
                      Synonyms and Secondary IDs (5)
                      Reported As
                      Symbol Synonym
                      CG3073
                      (Charng et al., 2014, Benos, 2000.12.13)
                      l(1)G0144
                      temp
                      (Graves et al., 2020, Salazar and Yamamoto, 2018, Hu et al., 2017.6.13, Charng et al., 2014, Yamamoto et al., 2013-)
                      Name Synonyms
                      lethal (1) G0144
                      tempura
                      (Graves et al., 2020, Charng et al., 2014)
                      Secondary FlyBase IDs
                      • FBgn0029609
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 36 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      References (34)