FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Mcm10
Open Close
General Information
Symbol
Dmel\Mcm10
Species
D. melanogaster
Name
Minichromosome maintenance 10
Annotation Symbol
CG9241
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032929
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Summary
Proposed to be involved in DNA replication and to participate in the activation of the pre-replication complex (pre-RC). May be involved in chromosome condensation. (UniProt, Q9VIE6)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Scim19

Key Links
Genomic Location
Cytogenetic map
39B1-39B1
Sequence location
Recombination map
2-54
RefSeq locus
NT_033779 REGION:21179933..21182812
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables DNA replication origin binding
inferred from biological aspect of ancestor with PANTHER:PTN000335708
(GO Reference Genome Project, 2011-)
enables double-stranded DNA binding
inferred from electronic annotation with InterPro:IPR040184
(InterPro Project Members, 2004-)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000335708
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR040184
(InterPro Project Members, 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from mutant phenotype
(Apger et al., 2010, Christensen and Tye, 2003)
involved_in DNA endoreduplication
inferred from mutant phenotype
(Apger et al., 2010)
involved_in DNA replication
inferred from mutant phenotype
(Christensen and Tye, 2003)
involved_in eye-antennal disc development
inferred from mutant phenotype
(Vo et al., 2014)
involved_in negative regulation of compound eye retinal cell apoptotic process
inferred from mutant phenotype
(Vo et al., 2014)
involved_in positive regulation of mitotic cell cycle
inferred from mutant phenotype
(Vo et al., 2014)
involved_in positive regulation of R7 cell differentiation
inferred from mutant phenotype
(Vo et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in DNA replication initiation
inferred from electronic annotation with InterPro:IPR040184
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000335708
(GO Reference Genome Project, 2011-)
involved_in DNA replication
inferred from electronic annotation with InterPro:IPR015408
(InterPro Project Members, 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Vo et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in nuclear replication fork
inferred from biological aspect of ancestor with PANTHER:PTN000335708
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR015408
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with UniProtKB:Q7L590
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM10 family. (Q9VIE6)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Proposed to be involved in DNA replication and to participate in the activation of the pre-replication complex (pre-RC). May be involved in chromosome condensation.
(UniProt, Q9VIE6)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Mcm10 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VIE6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm10-RA
FBtr0081475
NM_136253
2820
776
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mcm10-PA
FBpp0081004
86.5
776
9.86
Q9VIE6
NP_610097
AAF53976
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Self-associates. Interacts with Mcm2, dup, Orc2, CDC45L and Su(var)205.

(UniProt, Q9VIE6)
Domain

Each ZnF binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain. The ZnR also binds a zinc ion but is not involved in ssDNA or dsDNA binding.

(UniProt, Q9VIE6)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm10 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.09

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Vo et al., 2014)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm10 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm10
Transgenic constructs containing regulatory region of Mcm10
Aberrations (Deficiencies and Duplications) ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die during pupal stage, with Scer\GAL4Act5C.PI
partially lethal | maternal effect (with Df(2L)Exel6047)
partially lethal - majority die | recessive
viable
viable (with Df(2L)Exel6047)
Sterility
Allele
semi-sterile | female (with Df(2L)Exel6047)
Other Phenotypes
Allele
abnormal cell cycle | embryonic cycle | maternal effect (with Df(2L)Exel6047)
abnormal meiotic cell cycle | dominant
abnormal mitotic cell cycle | embryonic cycle 10
abnormal mitotic cell cycle | embryonic cycle 11
abnormal mitotic cell cycle | embryonic cycle 12
abnormal mitotic cell cycle | larval stage
abnormal mitotic cell cycle | larval stage (with Mcm10d08029)
abnormal mitotic cell cycle | larval stage (with Mcm10Scim19)
abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4GMR.PU
decreased body size | recessive
decreased fecundity | female (with Df(2L)Exel6047)
increased cell death | third instar larval stage, with Scer\GAL4GMR.PU
increased occurrence of cell division | third instar larval stage
some die during embryonic stage | maternal effect (with Df(2L)Exel6047)
suppressor of variegation | dominant
Phenotype manifest in
Allele
chromosome | embryonic stage (with Df(2L)Exel6047)
condensed nuclear chromosome
eye, with Scer\GAL4GMR.PU
eye disc, with Scer\GAL4GMR.PU
larval brain
nurse cell
ommatidium, with Scer\GAL4GMR.PU
ovariole (with Df(2L)Exel6047)
ovary (with Df(2L)Exel6047)
photoreceptor cell R7, with Scer\GAL4GMR.PU
photoreceptor cell R7 | third instar larval stage, with Scer\GAL4GMR.PU
polytene chromosome
polytene chromosome (with Mcm10d08029)
polytene chromosome (with Mcm10Scim19)
yolk (with Df(2L)Exel6047)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\MCM10
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Mcm10
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Mcm10
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\mcm10
11 of 13
Yes
Yes
Xtro\LOC101735259
1 of 13
No
Yes
Danio rerio (Zebrafish) (1)
Drer\mcm10
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\mcm-10
11 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP008723
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\MCM10
6 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\MCM10
4 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\cdc23
4 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mcm10. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM10; minichromosome maintenance 10 replication initiation factor
    14 of 14
    609357
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Self-associates. Interacts with Mcm2, dup, Orc2, CDC45L and Su(var)205.
    (UniProt, Q9VIE6 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-54
    Cytogenetic map
    39B1-39B1
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    39B1-39B1
    Limits computationally determined from genome sequence between P{lacW}l(2)k07215k07215&P{lacW}snRNA:U4:39Bk09410 and P{lacW}l(2)k14505k14505&P{lacW}l(2)k05106k05106
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    39B3-39B4
    (Dobie et al., 2001)
    Inferred from flanking sequence of Mcm101.
    (Dobie et al., 2001)
    Experimentally Determined Recombination Data
    Location

    2-54

    (FlyBase, 2016)

    2-54.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (9)
    Genomic Clones (20)
    cDNA Clones (107)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Vo et al., 2014)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments

          Maternally loaded Mcm10 protein is required for nuclear stability in early embryogenesis. It is also required for the development and/or maintenance of the female germline.

          (Reubens et al., 2015)

          Mcm10 interacts with members of the pre-RC and is required for proper chromosome condensation.

          (Christensen et al., 2002)

          Identification: In a screen for chromosome inheritance modifiers.

          (Dobie et al., 2001)
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: Mcm10 Scim19 CG9241

          (Christensen and Karpen, 2002.4.22)
          Additional comments

          "Scim19" may correspond to "CG9241" or "CG9242". Sequence analysis off ends of P{SUPor-P} in insertion mutant places "Scim19" near/in "CG9241" or "CG9242".

          (Dobie and Karpen, 2001.4.18)
          Nomenclature History
          Source for database identify of

          Source for identity of: Mcm10 CG9241

          (Christensen and Karpen, 2002.4.22)
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (9)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 41 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (54)