FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\eIF3m
Open Close
General Information
Symbol
Dmel\eIF3m
Species
D. melanogaster
Name
eukaryotic translation initiation factor 3 subunit m
Annotation Symbol
CG8309
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033902
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Summary
eukaryotic translation initiation factor 3 subunit m (eIF3m) encodes a non core component of the EIF3 translation initiation factor. The product of eIF3m functions during caspase mediated remodeling of the syncytial testis, where it interacts with the apoptosome. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Tango7

Key Links
Genomic Location
Cytogenetic map
50E1-50E1
Sequence location
Recombination map
2-69
RefSeq locus
NT_033778 REGION:14163066..14164825
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables caspase binding
inferred from physical interaction with FLYBASE:Dronc; FB:FBgn0026404,FLYBASE:Dark; FB:FBgn0263864
(D'Brot et al., 2013)
enables cysteine-type endopeptidase activator activity
inferred from direct assay
(D'Brot et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables translation initiation factor activity
inferred from electronic annotation with InterPro:IPR027528
(InterPro Project Members, 2004-)
enables translation initiation factor binding
inferred from sequence or structural similarity with MGI:MGI:1351744
(Gene Ontology Curators, 2002-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in Golgi organization
inferred from mutant phenotype
(Bard et al., 2006)
involved_in protein secretion
inferred from mutant phenotype
(Bard et al., 2006)
involved_in sperm individualization
inferred from mutant phenotype
(D'Brot et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in cytoplasmic translational initiation
inferred from biological aspect of ancestor with PANTHER:PTN000394291
(GO Reference Genome Project, 2011-)
involved_in translation
inferred from sequence or structural similarity with MGI:MGI:1351744
(Gene Ontology Curators, 2002-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Bard et al., 2006)
located_in Golgi apparatus
inferred from direct assay
(Bard et al., 2006)
located_in investment cone
inferred from direct assay
(D'Brot et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from electronic annotation with InterPro:IPR027528
(InterPro Project Members, 2004-)
part_of eukaryotic translation initiation factor 3 complex
inferred from biological aspect of ancestor with PANTHER:PTN000394291
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR027528
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with MGI:MGI:1351744
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the eIF-3 subunit M family. (Q7JVI3)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
eukaryotic translation initiation factor 3 subunit m (eIF3m) encodes a non core component of the EIF3 translation initiation factor. The product of eIF3m functions during caspase mediated remodeling of the syncytial testis, where it interacts with the apoptosome. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Protein Function (UniProtKB)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential).
(UniProt, Q7JVI3)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\eIF3m for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7JVI3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF3m-RA
FBtr0087579
NM_137088
1590
387
eIF3m-RB
FBtr0339964
NM_001299478
1386
387
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
eIF3m-PA
FBpp0086705
44.1
387
5.45
Q7JVI3
NP_610932
AAF58289
eIF3m-PB
FBpp0308985
44.1
387
5.45
Q7JVI3
NP_001286407
AHN56205
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

387 aa isoforms: eIF3m-PA, eIF3m-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, Q7JVI3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3m using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.02

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Bard et al., 2006)
located_in Golgi apparatus
inferred from direct assay
(Bard et al., 2006)
located_in investment cone
inferred from direct assay
(D'Brot et al., 2013)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3m in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF3m
Transgenic constructs containing regulatory region of eIF3m
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)Exel7130
(Ryder, 2004.4.26)
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\EIF3M
14 of 14
Yes
Yes
Hsap\COPS7A
1 of 14
No
No
Hsap\COPS7B
1 of 14
No
No
Hsap\PSMD13
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Eif3m
14 of 14
Yes
Yes
Rnor\Cops7a
1 of 14
No
No
Rnor\Cops7b
1 of 14
No
No
Rnor\Psmd13
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Eif3m
13 of 14
Yes
Yes
Mmus\Cops7a
1 of 14
No
No
Mmus\Cops7b
1 of 14
No
No
Mmus\Psmd13
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\eif3m
11 of 13
Yes
Yes
Xtro\cops7b
1 of 13
No
No
Xtro\smad2
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\eif3m
14 of 14
Yes
Yes
Drer\cops7a
1 of 14
No
No
Drer\psmd13
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\cif-1
13 of 14
Yes
Yes
Cele\rpn-9
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (3)
Agam\EIF3M_ANOGA
12 of 12
Yes
Yes
Agam\AgaP_AGAP001651
1 of 12
No
No
Agam\AgaP_AGAP012398
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\AT3G02200
12 of 13
Yes
Yes
Atha\AT5G15610
12 of 13
Yes
Yes
Atha\AT4G19006
1 of 13
No
No
Atha\AT5G45620
1 of 13
No
No
Atha\FUS5
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\RPN9
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\SPAC1751.03
11 of 12
Yes
Yes
Spom\csn71
1 of 12
No
No
Spom\rpn9
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:eIF3m. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
CSN7
3 of 13
Rpn9
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    eIF3mHMS00667
    exacerbates  cancer
    modeled by RbfHMS03004
    (Parkhitko et al., 2021)
    eIF3mHMS05756
    ameliorates  carcinoma
    modeled by EgfrUAS.cBa, Socs36EGD8929
    (Xu et al., 2022)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF3M; eukaryotic translation initiation factor 3 subunit M
    14 of 14
    609641
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        eIF3m - Dark
        anti tag coimmunoprecipitation, anti tag western blot
        (D'Brot et al., 2013)
        eIF3m - Dronc
        anti tag coimmunoprecipitation, anti tag western blot
        (D'Brot et al., 2013)
        eIF3m - Pdk1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - Pi3K92E
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - Pten
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - Rab11
        pull down, peptide massfingerprinting
        (Ramel et al., 2013)
        eIF3m - Rheb
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - S6kII
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - Sumo
        anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, western blot
        (Handu et al., 2015)
        eIF3m - eIF3a
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3b
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3c
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3e
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3f1
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3g1
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3h
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3k
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - eIF3l
        experimental knowledge based
        (Guruharsha et al., 2011)
        eIF3m - gig
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        eIF3m - sima
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Smn
        suppressible
        eIF3m
        (Sen et al., 2013)
        External Data
        Subunit Structure (UniProtKB)
        Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
        (UniProt, Q7JVI3 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-69
        Cytogenetic map
        50E1-50E1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        50E1-50E1
        Limits computationally determined from genome sequence between P{lacW}mamk05821 and P{lacW}l(2)s3475s3475&P{lacW}Hsc70-5k04907
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-69

        (FlyBase, 2016)

        2-74.5

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (11)
        Genomic Clones (16)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (136)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (D'Brot et al., 2013)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

            (Bard et al., 2006)

            dsRNA directed against Tango7 inhibits protein secretion in S2 cells and results in the Golgi membranes fragmenting into smaller elements in more than 50% of the treated cells.

            (Bard et al., 2006)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: CG8309 anon- EST:Posey76

            (Bayraktaroglu, 2000.11.2)

            Source for merge of: CG8309 anon- EST:Posey67

            (Ashburner, 2004.6.30)
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Tango7 CG8309

            (Bard et al., 2006)

            Source for identity of: eIF3m Tango7

            (Marygold et al., 2017)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (12)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 46 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (73)