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General Information
Name
AP-MS_PPI_InR-Tor
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0001699
Project
Data Provider
Title
Coaffinity purification of interacting proteins and identification by mass spectrometry, Kc167 cells, Insulin-Tor pathway components.
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Key genes
    Sample preparation

    Kc167 cells were transfected with one of 16 GFP-tagged proteins from the insulin receptor - target of rapamycin pathway. Cells were grown in medium containg 10% FBS, then starved (2% FBS) and treated with copper overnight (to induce transfected tagged bait proteins). Cells were either treated with 100nM insulin for 20 minutes before harvest or left untreated. All conditions were done in biological duplicate.

    Biosamples used (1)
    Biosample
    Type
    Title
    D. melanogaster, Kc167 cell line, stably transfected with bait protein, source for InR-Tor pathway affinity purifications.
    Assay Details
    Methods
    Key genes
    (bait_protein)
    Protocol

    Cells were lysed in the presence of DSP cross-linker and purified using anti-GFP beads.

    Mode of Assay

    Purified proteins were characterized by mass spectrometry.

    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    AP-MS_PPI_InR-Tor
    Name Synonyms
    Coaffinity purification of interacting proteins and identification by mass spectrometry, Kc167 cells, Insulin-Tor pathway components.
    Secondary FlyBase IDs
      References (2)