FlyBase Insertion Report: Dmel\TI{GAL4}dsx[KI.GAL4]

General Information

Symbol

Dmel\TI{GAL4}dsx^KI.GAL4

Species

D. melanogaster

Name

FlyBase ID

FBti0168641

Feature type

insertion_site

Also Known As

dsx-Gal4, dsx^GAL4

Description

Inserted Element

TI{GAL4}

LINE ID

Affected gene(s)

dsx

Viability / fertility

Associated allele(s)

dsx^KI.GAL4, Scer\GAL4^dsx.KI

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 84E5-84E6 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

84E5-84E6 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{GAL4}

(Rideout et al., 2010)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

dsx

(Chen et al., 2025, Chen et al., 2022, Jois et al., 2022, Peng et al., 2022, Massey et al., 2019, Grmai et al., 2018, Rideout et al., 2010)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

dsx^KI.GAL4

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^dsx.KI

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 15

gonad

(Rideout et al., 2010)

embryonic/larval oenocyte

(Rideout et al., 2010)

first instar larval stage | male

embryonic/larval oenocyte

(Rideout et al., 2010)

gonad

(Rideout et al., 2010)

first instar larval stage -- second instar larval stage

larval terminal neuroblast | subset

(Ghosh et al., 2019)

second instar larval stage

larval ventral nerve cord | restricted

(Rideout et al., 2010)

third instar larval stage

cell | subset of segment | segmentally repeated

(Rideout et al., 2010)

embryonic/larval fat body

(Rideout et al., 2010)

larval brain | restricted

(Rideout et al., 2010)

larval ventral nerve cord | restricted

(Rideout et al., 2010)

third instar larval stage | male

larval terminal neuroblast | subset

(Ghosh et al., 2019)

pupal stage P6 | male

gonad

Comment: 24h APF

(Rideout et al., 2010)

primordial germ cell

Comment: 24h APF

(Rideout et al., 2010)

pupal stage P6

pupal brain

Comment: 24h APF

(Rideout et al., 2010)

larval ventral nerve cord | restricted

Comment: 24h APF

(Rideout et al., 2010)

pharate adult stage P8 | male

gonad

Comment: 48h APF

(Rideout et al., 2010)

primordial germ cell

Comment: 48h APF

(Rideout et al., 2010)

pharate adult stage P8

pupal brain | restricted

Comment: 48h APF

(Rideout et al., 2010)

adult ventral nerve cord | restricted

Comment: 48h APF

(Rideout et al., 2010)

pharate adult stage P15(i) | male

gonad

Comment: 96h APF

(Rideout et al., 2010)

primordial germ cell

Comment: 96h APF

(Rideout et al., 2010)

adult stage | male

male genitalia

(Rideout et al., 2010)

adult fruitless pMP-e (female) neuron

Comment: referred to as pC1

(Rideout et al., 2010)

adult fruitless pIP-e (female) neuron

Comment: referred to as pC2

(Rideout et al., 2010)

tarsal segment | restricted

(Rideout et al., 2010)

adult gnathal ganglion | restricted

(Rezaval et al., 2012)

adult ventral nerve cord | restricted

(Rezaval et al., 2012)

adult fruitless P1 (male) neuron | subset

(Pavlou et al., 2016)

adult fruitless pIP-e (male) neuron | subset

(Pavlou et al., 2016)

adult doublesex pCd (male) neuron

(Pavlou et al., 2016)

adult doublesex SN (male) neuron

(Pavlou et al., 2016)

adult doublesex TN1 (male) neuron

(Pavlou et al., 2016)

adult doublesex TN2 (male) neuron

(Pavlou et al., 2016)

adult abdominal doublesex (male) neuron

(Pavlou et al., 2016)

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^dsx.KI drives expression in four of twelve neuroblasts in the larval terminal abdominal neuromere; in female larvae, these neuroblasts die during mid-third instar.

(Ghosh et al., 2019)

Drives expression in doublesex-expressing neurons and neuroblasts, and in oenocytes, at all developmental stages.

(FlyBase Curators, 2017)

ScerGAL4^dsx.KI drives expression in non-neuronal tissues, including unidentified sub- and peri-cuticular cells.

(Pavlou et al., 2016)

ScerGAL4^dsx.KI drives expression in both male and female polyploid enterocytes, but not in esg-positive precursors, or enteroendocrine cells.

(Hudry et al., 2016)

ScerGAL4^dsx.KI drives expression in the posterior part of the thoracico-abdominal ganglion and a subset of neurons in the adult brain. In males expression in the brain is stronger than in females and additional expression in more anterior parts of the thoracico-abdominal ganglion are detected.

(Rezával et al., 2014)

ScerGAL4^dsx.KI drives expression in 3 clusters of neurons in the adult female genital tract: one cluster of 1 neuron in the lateral oviduct (which co-localises with AvicGFP^1.E.ppk) and two clusters in the uterus. An anterior one with 3 neurons (which also express AvicGFP^1.E.ppk) and one more posterior with 7 neurons which co-express AvicGFP^1.E.ppk, with 3 neurons of those also expressing EcollexA^{fru.P1.T:HsimVP16}. In combination with ScerFLP1²⁵⁰, ScerGAL4^dsx.KI drives expression in 27, out of a total of 300 neurons, in the abdominal ganglion of adult females. These neurons include four ascending abdominal ganglion neurons that terminate in the subesophageal ganglion, and two subesophageal ganglion descending neurons that innervate the uterus. Sex specific differences were observed, with the majority of males displaying only one ascending neuron, and half of the males displaying two descending neurons.

(Rezaval et al., 2012)

ScerGAL4^dsx.KI drives expression in several cell clusters in the CNS throughout development, with some of the cell clusters being either male specific (SN, TN1 and TN2), or composed of more cells in males. In the adult CNS, expression is observed in 900 neurons in males (9 clusters) and 700 neurons in females (6 clusters). Co-localisation with fru protein is observed in some clusters including pC1, pC2, pC3 and in the abdominal ganglion.

(Rideout et al., 2010)

Marker for

Reflects expression of

dsx

(Ghosh et al., 2019, Hudry et al., 2016, Pavlou et al., 2016, Rezával et al., 2014, Rezaval et al., 2012, Rideout et al., 2010)

Reporter construct used in assay

Ecol\lacZ^{UAS.Tag:NLS(SV40-largeT)}

(Rideout et al., 2010)

Mmus\Cd8a^UAS.cLa.EGFP

(Rezával et al., 2014, Rideout et al., 2010)

Disc\RFP^{DsRedT4.UAS.Tag:NLS(tra)}

(Rezaval et al., 2012, Rideout et al., 2010)

nSyb^{FRT.UAS.cYa.GFP}

(Rezaval et al., 2012)

Mmus\Cd8a^{FRT.UAS.cYa.GFP}

(Rezával et al., 2014, Rezaval et al., 2012)

Mmus\Cd8a^UAS.cYa.GFP

(Rezaval et al., 2012)

Scer\FLP1²⁵⁰

(Rezaval et al., 2012)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Dsx-Gal4

(Jois et al., 2022, Deutsch et al., 2020)

Dsx-gal4^3'UTR-

(Wang and Yoder, 2012)

TI{GAL4}dsx^KI.GAL4

(FlyBase, 1992-)

dsx-GAL4

(Singh et al., 2025, Marie et al., 2023, Wainwright et al., 2021, Fan et al., 2020, Ghosh et al., 2019, Redhai et al., 2016, Clough et al., 2014, Wang et al., 2013)

dsx-Gal4

(Hérault et al., 2024, Riva et al., 2022, Chiu et al., 2021, Zhou et al., 2021, Bakshi et al., 2020, Deutsch et al., 2019, Romero-Pozuelo et al., 2019, Zhang et al., 2019, Chiang et al., 2016, Hudry et al., 2016, Kimura et al., 2015, Kohatsu and Yamamoto, 2015)

dsx-gal4

(Grmai et al., 2018)

dsx^GAL4

(Han et al., 2022, Han et al., 2022, Peng et al., 2022, Ishii et al., 2020, Massey et al., 2019, Sato et al., 2019, Pavlou et al., 2016)

dsx^Gal4

(Pacheco et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (43)

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