Reference Report

Reference
Citation	Yang, S.Y., Baxter, E.M., Van Doren, M. (2012). Phf7 controls male sex determination in the Drosophila germline. Dev. Cell 22(5): 1041--1051. (Export to RIS)
FlyBase ID	FBrf0218344
Publication Type	Research paper
PubMed ID	22595675
PubMed Abstract	Establishment of germline sexual identity is critical for production of male and female germline stem cells, as well as sperm versus eggs. Here we identify PHD Finger Protein 7 (PHF7) as an important factor for male germline sexual identity in Drosophila. PHF7 exhibits male-specific expression in early germ cells, germline stem cells, and spermatogonia. It is important for germline stem cell maintenance and gametogenesis in males, whereas ectopic expression in female germ cells ablates the germline. Strikingly, expression of PHF7 promotes spermatogenesis in XX germ cells when they are present in a male soma. PHF7 homologs are also specifically expressed in the mammalian testis, and human PHF7 rescues Drosophila Phf7 mutants. PHF7 associates with chromatin, and both the human and fly proteins bind histone H3 N-terminal tails with a preference for dimethyl lysine 4 (H3K4me2). We propose that PHF7 acts as a conserved epigenetic "reader" that activates the male germline sexual program.
DOI	10.1016/j.devcel.2012.04.013
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (2)
	Df(1)Phf7^Δ88 Df(3L)st-j7
Alleles (18)
	Ecol\lacZ^esg-M5-4 Hsap\PHF7^{Scer\UAS.P\T.cYa} otu¹ Phf7^{3xC.T:Ivir\HA1} Phf7^{3xN.T:Ivir\HA1} Phf7^EY03023 Phf7^{Scer\UAS.P\T.cYa} Phf7^{Scer\UAS.P\T.T:Ivir\HA1} Phf7^{T:Zzzz\FLAG,T:Zzzz\StrepII,T:Zzzz\His6} Phf7^ΔN2 Phf7^ΔN18 Scer\GAL4^{bam.T:Hsim\VP16} Scer\GAL4^C587 Scer\GAL4^fkh.PH Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^Scer\UAS.cHa tra¹ tra^F.U2af50
Constructs (11)
	P{bam-GAL4:VP16} P{fkh-GAL4.H} P{GAL4::VP16-nos.UTR} P{U2af50-tra^F} P{UAS-Gal4.H} P{UASp-hPhf7.Y} P{UASp-Phf7.HA} P{UASp-Phf7.Y} PBac{3xFlag-Strep-6xHis-Phf7} PBac{3xHA-Phf7} PBac{Phf7-3xHA}
Genes (17)
	bam CadN Ecol\lacZ esg His3 Hsap\PHF7 hts otu Phf7 Rab35 Scer\GAL4 T:Ivir\HA1 T:Zzzz\FLAG T:Zzzz\His6 T:Zzzz\StrepII tra vas
Insertions (2)
	P{EPgy2}Phf7^EY03023 P{GAL4}C587
Natural transposons (2)
	P-element Tni\piggyBac