[63F1-63F1];[64A4-64A4];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
63F1;64A4
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3L)BSC369/Df(3L)BSC368 mutant embryos show high frequency of defects in the salivary gland lumen (including variations in lumen diameter, bending/folding or occasional branching of the lumen) compared to wild-type controls.
The presence of P+PBac{XP5.WH5}BSC368 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of the progenitor PBac{WH}f01986 to be at Release 3 genomic coordinate 3740450 on chromosome arm 3L. This corresponds to 63F1 on the Release 3 and 5genome map. The predicted position of the progenitor P{XP}CG11593d06001 on the Release 5 map is 64A4. Consequently, the cytological breakpoints of Df(3L)BSC368 are predicted to be 63F1;64A4.