FlyBase Allele Report: Dmel\N[Ax-1]

General Information

Symbol

Dmel\N^Ax-1

Species

D. melanogaster

Name

FlyBase ID

FBal0012850

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Ax²⁸, Ax¹, Ax^28a, N^Ax1, N^Ax, N^Ax28, Ax

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

gain of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(de Celis and Garcia-Bellido, 1994, de Celis et al., 1991, Mari-Beffa et al., 1991)

gain of function allele

(Royet et al., 1998, de Celis, 1997, de Celis et al., 1997, de Celis et al., 1996, Mari-Beffa et al., 1991)

Mutagen

spontaneous

(Lindsley and Zimm, 1992)

Progenitor genotype

Cytology

A single-band duplication, presumed to be for 3C7 by Schultz (FBrf0005574). Lefevre et al., (FBrf0009019), on the other hand, argue against a duplication for 3C7 on the basis of equal X-ray-induced mutability to N in 'Ax' and +. Molecular information also incompatible with the presence of a duplication.

(Lindsley and Zimm, 1992)

Description

Amino acid replacement: N986I. N986 lies in EGF repeat 25. Has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (FBrf0045770).

(Lindsley and Zimm, 1992)

Amino acid replacements in the EGF-homologous regions of the extracellular domain of the N protein.

(de Celis et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,165,585..3,165,585 [+]

Nucleotide change:

A3165585T

Amino acid change:

N986I | N-PA; N986I | N-PB

Reported amino acid change:

N986I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lindsley and Zimm, 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity

(Fanto and Mlodzik, 1999)

lethal | recessive

(Kramers et al., 1983)

lethal | recessive | heat sensitive

(Lindsley and Zimm, 1992, Portin and Siren, 1976)

partially lethal (with N^Ax-16)

(Grushko et al., 1998)

partially lethal (with N^Ax-71d)

(Grushko et al., 1998)

partially lethal (with N^Ax-E2)

(Grushko et al., 1998)

viable

(de Celis and Bray, 2000, Grushko et al., 1998, de Celis et al., 1996)

viable (with N^Ax-9)

(Grushko et al., 1998)

visible

(Thumm and Kadowaki, 2001, Adachi-Yamada et al., 1999, Grushko et al., 1998, Palka et al., 1990)

visible | dominant

(Guo et al., 1999, Royet et al., 1998)

visible | heat sensitive

(Thompson et al., 1988)

visible | recessive | heat sensitive

(Lindsley and Zimm, 1992)

Phenotype Manifest In

Detailed Description

Statement

Reference

When grown at 25^oC, mutants lack the distal most parts of wing vein L5 band L4.

(Morey et al., 2001)

N^Ax-1 flies have mild eye-polarity defects; ommatidia are sometimes symmetrical, having an R4/R4-like phenotype.

(Fanto and Mlodzik, 1999)

Wing vein L2 is absent and wing vein L5 is interrupted.

(Guo et al., 1999)

N^Ax-1/N^Ax-16 larvae have enlarged wing discs compared to wild-type. This phenotype is associated with elevated mitotic activity in the disc, particularly in the peripheral region of the wing pouch.

(Go et al., 1998)

Viable in combination with N^Ax-9.

(Grushko et al., 1998)

The number of missing bristles increases with increasing temperature in homozygous flies, while the number and length of wing vein breaks increases from 18^oC to 25^oC and then decreases again from 25^oC to 29^oC.

(Grushko et al., 1998)

Reduced number of bristles on the head and thorax and shortening of wing veins.

(Royet et al., 1998)

Homozygotes form wing vein gaps.

(Miyamoto et al., 1995)

Thickened vein mutant.

(Sturtevant and Bier, 1995)

Length of macrochaetae is reduced with respect to that of wild type. Suppresses the N haplo-insufficient phenotype of loss of the wing margin.

(de Celis and Garcia-Bellido, 1994)

Neural precursors never form in the Ax class of N alleles. Cells mutant for Ax class alleles but with some neural potential are inhibited from becoming neural by their neighbours but do not themselves affect their neighbours, which can become neural.

(Heitzler and Simpson, 1993)

sgg³² is epistatic to N^Ax-1.

(Ruel et al., 1993)

Male viable at 25^oC but nearly lethal at 29^oC. N^Ax-1/N^Ax-E2 semi-lethal at 25^oC and lethal at 29^oC. N^Ax-1/+ females show short L5 in half of the flies and sparse hair pattern on thorax. Lower temperature (19^oC) markedly decreases expression, and higher temperature enhances it. Some Ax alleles enhance N expression in N^Ax-1/N heterozygotes, but others suppress the dominant N phenotype. For example, N^Ax-1/Df(1)N-8 approaches wild type in all characteristics. No wing-vein interruption in N^Ax-1/+ at 18^oC and 26^oC and enhancement by H¹ occurs. N^Ax-1/N^Ax-1; ci^D/+ and N^Ax-1/Y; ci^D/+ are lethal or nearly so at 26^oC. At 22^oC, males survive and show enhanced wing-vein interruption and more missing bristles. Wing nicking is suppressed in N^Ax-1/N^55e11 at 25^oC and Ax venation is weakly expressed. In heterozygotes of N^Ax-1 with the recessives at N at 18^oC and 25^oC, there is neither expression of the recessive nor Ax-type venation. Temperature-sensitive period for lethality of N^Ax-1 at beginning of pupal stage; of N^Ax-1/N^Ax-E2 at end of third instar and into early pupal stage.

(Lindsley and Zimm, 1992)

Phenotypes have topographic pattern specificities.

(Mari-Beffa et al., 1991)

Lack of chaetae. N^Ax-1 and N^Ax-16 correct Hw phenotypes in both ectopic and normal positions.

(de Celis et al., 1991)

Wing phenotype is enhanced by mutations at da.

(Brand and Campos-Ortega, 1990)

Some hemizygous males occasionally lack the twin sensilla of the wing margin, the ventral sensillum of wing vein L3, and the anterior cross vein sensillum.

(Palka et al., 1990)

The L5 wing vein phenotype is more severe at 25^oC than 20^oC, the temperature-sensitive period is between 10 and 15% of pupal development time.

(Thompson et al., 1988)

All heterozygotes with facet or notchoid alleles except N^Ax-1/N^nd-2 show some Ax venation.

(Portin, 1977)

Nearly lethal when reared at 29^oC; temperature-sensitive period early pupa.

(Portin and Siren, 1976)

N^Ax-1/N^Ax-1; Dp(1;2)51b/+ shows weak Ax venation.

(Portin, 1975)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Ax-1/N^Ax-E2 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-1/N^Ax-E2 has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

N^Ax-71d/N^Ax-1 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-71d/N^Ax-1 has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

N^Ax-1/N^Ax-16 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2

(Grushko et al., 1998)

N^Ax-1/N^Ax-16 has partially lethal phenotype, enhanceable by Dp(3;3)bxd¹¹⁰

(Grushko et al., 1998)

Suppressed by

Statement

Reference

N^Ax-1 has visible phenotype, suppressible by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

NOT Enhancer of

Statement

Reference

N^Ax-1 is a non-enhancer of visible phenotype of E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

Suppressor of

Statement

Reference

N^Ax-1/N[+] is a suppressor of visible | dominant phenotype of fng^D4

(Dansereau et al., 2002)

N^Ax-1 is a suppressor of visible phenotype of E(spl)m4-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

NOT Suppressor of

Statement

Reference

N^Ax-1 is a non-suppressor of visible phenotype of E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

Other

Statement

Reference

Dp(3;3)bxd¹¹⁰, N^Ax-1 has partially lethal phenotype

(Grushko et al., 1998)

Dp(3;3)MKRS-D2, N^Ax-1 has partially lethal phenotype

(Grushko et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^Ax-1 has orbital bristle phenotype, enhanceable by Nle[+]/Nle^k13714

(Royet et al., 1998)

N^Ax-1 has dorsocentral bristle phenotype, enhanceable by Nle[+]/Nle^k13714

(Royet et al., 1998)

N^Ax-1 has wing vein phenotype, enhanceable by Nle[+]/Nle^k13714

(Royet et al., 1998)

N^Ax-1 has orbital bristle phenotype, enhanceable by Nle[+]/Nle^Δ8

(Royet et al., 1998)

N^Ax-1 has dorsocentral bristle phenotype, enhanceable by Nle[+]/Nle^Δ8

(Royet et al., 1998)

N^Ax-1 has wing vein phenotype, enhanceable by Nle[+]/Nle^Δ8

(Royet et al., 1998)

N^Ax-1 has wing vein phenotype, enhanceable by Scer\GAL4^ap-md544/Nle^UAS.cRa

(Royet et al., 1998)

N^Ax-1 has dorsocentral bristle phenotype, enhanceable by Scer\GAL4^ap-md544/Nle^UAS.cRa

(Royet et al., 1998)

N^Ax-1 has orbital bristle phenotype, enhanceable by Scer\GAL4^ap-md544/Nle^UAS.cRa

(Royet et al., 1998)

N^Ax-1 has wing vein L5 phenotype, enhanceable by ab¹

(Thompson et al., 1988)

NOT Enhanced by

Statement

Reference

N^Ax-1 has wing vein L4 phenotype, non-enhanceable by Sps1^k11320

(Morey et al., 2001)

N^Ax-1 has wing vein L5 phenotype, non-enhanceable by Sps1^k11320

(Morey et al., 2001)

Suppressed by

Statement

Reference

N^Ax-1 has wing vein phenotype, suppressible by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

N^Ax-1 has wing vein L2 phenotype, suppressible by Psn^W11

(Guo et al., 1999)

N^Ax-1 has wing vein L5 phenotype, suppressible by Psn^W11

(Guo et al., 1999)

N^Ax-1 has wing vein L2 phenotype, suppressible by Psn^W6

(Guo et al., 1999)

N^Ax-1 has wing vein L5 phenotype, suppressible by Psn^W6

(Guo et al., 1999)

N^Ax-1 has wing vein phenotype, suppressible by cno^mis1

(Miyamoto et al., 1995)

NOT suppressed by

Statement

Reference

N^Ax-1 has wing vein L4 phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

N^Ax-1 has wing vein L5 phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

N^Ax-1 has wing phenotype, non-suppressible by p38b^DN.UAS/Scer\GAL4^unspecified

(Adachi-Yamada et al., 1999)

Enhancer of

Statement

Reference

N^Ax-1 is an enhancer of phenotype of H^unspecified

(Mari-Beffa et al., 1991)

N^Ax-1 is an enhancer of phenotype of emc^D

(Mari-Beffa et al., 1991)

Suppressor of

Statement

Reference

N^Ax-1/N[+] is a suppressor of wing margin phenotype of fng^D4

(Dansereau et al., 2002)

N^Ax-1/N[+] is a suppressor of wing margin phenotype of Scer\GAL4^Bx-MS1096, rho^Neo.UAS

(Guichard et al., 2002)

N^Ax-1/N[+] is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, rho^Neo.UAS

(Guichard et al., 2002)

N^Ax-1/N[+] is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, rho^Neo.UAS

(Guichard et al., 2002)

N^Ax-1 is a suppressor of scutellar bristle phenotype of E(spl)m4-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

N^Ax-1 is a suppressor of wing vein phenotype of tkv¹/tkv⁸

(de Celis, 1997)

N^Ax-1 is a suppressor of phenotype of emc^unspecified

(Mari-Beffa et al., 1991)

Other

Statement

Reference

N^Ax-1, dpp^s4/dpp^s8 has wing vein phenotype

(de Celis, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

CG6194^P0997/CG6194^P0997 suppresses the loss of wing vein seen in N^Ax-1/Y flies.

(Thumm and Kadowaki, 2001)

Expression of p38b^DN.Scer\UAS under the control of Scer\GAL4^unspecified has no significant effect on the wing phenotype caused by N^Ax-1.

(Adachi-Yamada et al., 1999)

N^Ax-1 suppresses the supernumerary scutellar bristle phenotype seen in flies with m4^Scer\UAS.cAa driven by Scer\GAL4^455.2. The addition of N^Ax-1 reduces the number of supernumerary scutellar bristles seen in flies with mα^Scer\UAS.cAa driven by Scer\GAL4^455.2, though there are still more bristles seen than in wild-type.

(Apidianakis et al., 1999)

The wing vein phenotypes of heterozygotes are partially suppressed by Psn^W6 and Psn^W11.

(Guo et al., 1999)

Homozygous viability is decreased if the flies also carry Dp(3;3)MKRS-D2 or Dp(3;3)bxd¹¹⁰.

(Grushko et al., 1998)

The variation of the number of missing bristles and number and length of wing vein breaks with temperature in N^Ax-1; E(spl)¹ double mutants is the same as in E(spl)¹ single mutants.

(Grushko et al., 1998)

dpp^s4/dpp^s8 N^Ax-1 or tkv¹/tkv⁸ N^Ax-1 double mutant flies show pronounced wing vein loss. The thickening of veins seen in tkv¹/tkv⁸ flies is suppressed in combination with N^Ax-1.

(de Celis, 1997)

The wing vein gaps of N^Ax-1 homozygotes are dominantly suppressed by cno^mis1.

(Miyamoto et al., 1995)

The L5 wing vein phenotype is more extreme in N^Ax-1 ab¹ double mutants, and there is a deep middorsal furrowing of the thorax (a trait characteristic of some Abruptex alleles), suggesting that ab¹ acts as an enhancer of the N^Ax-1 phenotype.

(Thompson et al., 1988)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

N^264-8

(Slizynska, 1938)

N^264-39

(Slizynska, 1938)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Nazarenko, Jan. 1928.

(Lindsley and Zimm, 1992)

Comments

N^Ax-1 shows negative complementation with N^Ax-M1 and N^Ax-E2.

(de Celis and Garcia-Bellido, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

(Kramers et al., 1983, Slizynska, 1938)

Ax¹

(Adachi-Yamada et al., 1999, Matsuo et al., 1995, Brand and Campos-Ortega, 1990, Thompson et al., 1988)

Ax²⁸

(de Celis and Bray, 2000, Grushko et al., 1998, Grushko et al., 1998, Royet et al., 1998, de Celis, 1997, de Celis et al., 1997, de Celis et al., 1996, Gomez-Skarmeta and Modolell, 1996, de Celis et al., 1995, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, Heitzler and Simpson, 1993, de Celis et al., 1991)

Ax^28a

(Go et al., 1998, Mari-Beffa et al., 1991, Palka et al., 1990)

Dp(1;1)Ax28a

Dp(1;1)Ax^28a

N^Ax-1

(Guichard et al., 2013)

N^Ax-28a

N^Ax1

(Guichard et al., 2002, Thumm and Kadowaki, 2001)

N^Ax28

(Apidianakis et al., 1999, Fanto and Mlodzik, 1999)

N^Ax

(Apidianakis et al., 1999, Sturtevant and Bier, 1995)

Name Synonyms

Secondary FlyBase IDs

FBab0003034
FBal0029937

References (44)

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