FlyBase Allele Report: Dmel\fj[d1]

General Information

Symbol

Dmel\fj^d1

Species

D. melanogaster

Name

FlyBase ID

FBal0049500

Feature type

allele

Associated gene

Dmel\fj

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Zeidler et al., 1999, Brodsky and Steller, 1996)

Mutagen

Delta2-3 transposase

(Brodsky and Steller, 1996)

Progenitor genotype

fj^p2

(Brodsky and Steller, 1996)

Cytology

Description

All but the last 57 amino acids of the coding region are deleted, with no remaining in frame methionines.

(Zeidler et al., 1999)

Deletion of approximately 4kb extending into fj transcribed region and removing at least the first 100 amino acids including a putative signal sequence.

(Brodsky and Steller, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:18,231,684..18,235,684 [+]

Comment:

FBrf0086339 : Deletion of approximately 4kb extending into fj transcribed region and removing at least the first 100 amino acids including a putative signal sequence. FBrf0123257 : All but the last 57 amino acids of the coding region are deleted, with no remaining in frame methionines. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Zeidler et al., 1999, Brodsky and Steller, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 2 & macrochaeta

(Zeidler et al., 2000)

abdominal segment 2 & microchaeta

(Zeidler et al., 2000)

abdominal tergite | cell non-autonomous | somatic clone

(Casal et al., 2002)

adult cuticle | cell non-autonomous | somatic clone

(Casal et al., 2002)

embryonic/larval tracheal dorsal trunk

(Chung et al., 2009)

eye

(Brodsky and Steller, 1996)

eye (with Df(2R)Pcl11B)

(Zeidler et al., 1999)

eye | somatic clone

(Zeidler et al., 1999)

leg

(Zeidler et al., 2000, Brodsky and Steller, 1996)

macrochaeta | cell non-autonomous | somatic clone

(Casal et al., 2002)

metatarsus

(Brodsky and Steller, 1996)

microchaeta | cell non-autonomous | somatic clone

(Casal et al., 2002)

ommatidium (with Df(2R)Pcl11B)

(Zeidler et al., 1999)

ommatidium | cell non-autonomous | somatic clone, with fj^{t14.Tag:Golgi(hGALNT3)}

(Strutt et al., 2004)

ommatidium | somatic clone

(Zeidler et al., 1999)

ommatidium | somatic clone, with fj^{t14.Tag:Golgi(hGALNT3)}

(Strutt et al., 2004)

tarsal segment 2

(Zeidler et al., 2000, Brodsky and Steller, 1996)

tarsal segment 3

(Zeidler et al., 2000, Brodsky and Steller, 1996)

trichome & tergite | cell non-autonomous | somatic clone

(Brittle et al., 2010)

wing

(Zeidler et al., 2000, Brodsky and Steller, 1996)

wing | distal

(Zeidler et al., 2000)

wing blade

(Hogan et al., 2011)

wing hair

(Zeidler et al., 2000)

wing hair | cell autonomous | somatic clone

(Zeidler et al., 2000)

wing hair | cell non-autonomous | somatic clone

(Zeidler et al., 2000)

wing vein | cell autonomous | somatic clone

(Zeidler et al., 2000)

wing vein | cell non-autonomous | ectopic | somatic clone

(Zeidler et al., 2000)

wing vein | cell non-autonomous | somatic clone

(Zeidler et al., 2000)

Detailed Description

Statement

Reference

Homozygous fj^d1 wings have altered shape, but hair polarity is disrupted in only a small proximal region. Posterior ridges of fj^d1 homozygous wings also have a more anterior-posterior orientation than wild-type.

(Hogan et al., 2011)

Homozygous clones in the dorsal adult abdomen reverse hair polarity behind the clone.

(Brittle et al., 2010)

Hemizygous stage 16 embryos have longer dorsal trunks than normal.

(Chung et al., 2009)

An equator can easily be discerned in fj^d1/fj^N7 eyes. 98.5% of ommatidia are correctly constructed and rotated in these animals. Flies expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a fj^d1/fj^N7 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 92.9% of ommatidia are correctly constructed and rotated in these animals. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb3-GAL4 in a fj^d1/fj^N7 background has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen.

(Simon, 2004)

Somatic clones lacking fj^{t14.T:Hsap\GALNT3} induced in a fj^d1/fj^d1; fj^{t14.T:Hsap\GALNT3}/+ background non-autonomously cause inversion of ommatidia.

(Strutt et al., 2004)

Within the anterior compartments of the adult abdominal cuticle, somatic clones of fj^d1 only exhibit planar polarity defects when they are located in approximately the front half of the compartment. Within affected fj^d1 clones, the rows of hairs are jumbled and hair orientation is disturbed: most of those at the back of the clone are reversed, as are the wild-type hairs behind it. The further anterior the clone, the more disturbance within and the more reversal of hair polarity behind. Some somatic clones of fj^d1 in the anterior (hair producing) territory of the posterior compartment form dishevelled and incorrectly polarized hairs; however, most appear normal. These clones do not have non-autonomous effects.

(Casal et al., 2002)

The normal regular pattern of wing hairs pointing towards the distal end of the wing is essentially unchanged in homozygous mutant individuals, occasional minor defects in wing hair polarity are identified. Homozygous mutant clones in the wing produce striking disruptions in the normal pattern of wing hair polarity. This effect wherever clones appeared in the wing. Occasionally loss of wing hairs is seen within the presumptive mutant area. Sometimes striking cell non-autonomous effects are seen on the proximal side of mutant patches. Clones smaller than about 5x5 cells never give non-autonomous phenotypes. All clones larger than about 5x5 cells produce non-autonomous phenotypes in a region distal to the posterior cross vein. Larger clones also produce strong non-autonomous phenotypes in a region between veins 3 and 4, and weaker phenotypes are observed in a large region in the centre of the wing posterior to vein 4. Clones sometimes contain veins that have either been duplicated or diverted, so that they remain in wild-type tissue immediately adjacent to the clone. In addition ectopic regions of wing vein material are found in many cones and occasional mutant clones are found in which veins fade after entering mutant tissue. In homozygous mutant adults flies, hair polarity defects and hair swirls are seen in the a2 region about 50% of the time. When homozygous mutant clones are produced in the abdomen, in a2 and the anterior of a3, both cell-autonomous and non-autonomous planar polarity defects are seen. Approximately three rows of non-autonomous hair inversions are seen at the posterior side of clone, and major disruptions of hair polarity are seen within the clone. No phenotypes are seen when clones are generated in the rest of the abdomen. Legs from fj^d1 homozygotes are shortened and display the characteristic fusion of the second and third tarsi. Wings from fj^d1 homozygotes are shortened - specifically the region between the anterior and the posterior crossvein is significantly reduced, whilst the proximal wing regions are unaffected.

(Zeidler et al., 2000)

fj^d1/Df(2R)Pcl11B flies, exhibit occasional ommatidia with inverted polarity relative to their neighbours. In homozygous fj^d1 somatic clones in the eye produce strong effects on dorsoventral ommatidial patterning. Clones lying close to the equator in either hemisphere of the eye result in dorsoventral inversions of polarity in 1-3 rows straddling the boundary of the clone furthest from the equator (polar boundary). This is non-autonomous as ommatidia beyond the polar boundary of clones were inverted. The closer the clone is to the equator the stronger the phenotype is seen. The phenotype tended to be stronger at the posterior ends of clones. Clones that straddle the equator have inverted ommatidia at both polar edges, but the equator remains.

(Zeidler et al., 1999)

Legs are shorter than wild type with four instead of five tarsal segments. The first three but not the fourth or the fifth tarsal segments are affected. The wing is reduced in length. Eye surface is deformed, though this phenotype is only evident for stronger alleles and has variable penetrance and expressivity.

(Brodsky and Steller, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fj^d1 has abnormal planar polarity phenotype, enhanceable by ds¹/ds^UAO71

(Strutt et al., 2004)

Enhancer of

Statement

Reference

fj^d1/fj^d1 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^salm-459.2, ds^UAS.cTa

(Matakatsu and Blair, 2004)

fj^d1/fj^d1 is an enhancer of abnormal planar polarity phenotype of ds¹/ds^UAO71

(Strutt et al., 2004)

NOT Enhancer of

Statement

Reference

fj^d1/fj^d1 is a non-enhancer of abnormal planar polarity phenotype of ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Suppressor of

Statement

Reference

fj^d1/fj^d1 is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^en-e16E, ft^UAS.cMa

(Matakatsu and Blair, 2004)

NOT Suppressor of

Statement

Reference

fj^d1/fj^d1 is a non-suppressor of abnormal planar polarity phenotype of ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Other

Statement

Reference

fj^d1, pk³⁰ has visible phenotype

(Hogan et al., 2011)

fj^d1, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

ds^38k/ds^UAO71, fj^N7/fj^d1 has abnormal planar polarity phenotype

(Simon, 2004)

ds¹/ds^UAO71, fj^d1 has abnormal planar polarity phenotype

(Strutt et al., 2004)

Df(2R)Pcl11B, fj^d1 has abnormal planar polarity phenotype

(Zeidler et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

fj^d1 has crossvein phenotype, enhanceable by ds¹/ds[+]

(Strutt et al., 2004)

fj^d1 has wing phenotype, enhanceable by ds¹/ds[+]

(Strutt et al., 2004)

fj^d1 has crossvein phenotype, enhanceable by ds^38k/ds[+]

(Strutt et al., 2004)

fj^d1 has wing phenotype, enhanceable by ds^38k/ds[+]

(Strutt et al., 2004)

fj^d1 has crossvein phenotype, enhanceable by ft¹/ft[+]

(Strutt et al., 2004)

fj^d1 has wing phenotype, enhanceable by ft¹/ft[+]

(Strutt et al., 2004)

fj^d1 has crossvein phenotype, enhanceable by ft¹/ft¹

(Strutt et al., 2004)

fj^d1 has wing phenotype, enhanceable by ft¹/ft¹

(Strutt et al., 2004)

fj^d1 has wing phenotype, enhanceable by ds¹/ds^UAO71

(Strutt et al., 2004)

Enhancer of

Statement

Reference

fj^d1/fj[+] is an enhancer of embryonic/larval cuticle phenotype of baz⁴

(Shao et al., 2010)

fj^d1/fj[+] is an enhancer of embryonic/larval cuticle phenotype of baz^G0484

(Shao et al., 2010)

fj^d1/fj^d1 is an enhancer of wing hair phenotype of Scer\GAL4^salm-459.2, ds^UAS.cTa

(Matakatsu and Blair, 2004)

fj^d1/fj^d1 is an enhancer of wing phenotype of ds¹/ds^UAO71

(Strutt et al., 2004)

fj^d1/fj^d1 is an enhancer of crossvein phenotype of ft¹

(Strutt et al., 2004)

fj^d1/fj^d1 is an enhancer of wing phenotype of ft¹

(Strutt et al., 2004)

NOT Enhancer of

Statement

Reference

fj^d1/fj^d1 is a non-enhancer of wing hair phenotype of ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Suppressor of

Statement

Reference

fj^d1/fj^d1 is a suppressor of wing hair | posterior phenotype of Scer\GAL4^en-e16E, ft^UAS.cMa

(Matakatsu and Blair, 2004)

fj^d1/fj^d1 is a suppressor of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, ft^UAS.cMa

(Matakatsu and Blair, 2004)

NOT Suppressor of

Statement

Reference

fj^d1/fj^d1 is a non-suppressor of wing hair phenotype of ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Other

Statement

Reference

fj^d1, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

ds^38k/ds^UAO71, fj^N7/fj^d1 has wing blade phenotype

(Simon, 2004)

ds¹/ds^UAO71, fj^d1 has ommatidium phenotype

(Strutt et al., 2004)

ds¹/ds^UAO71, fj^d1 has wing hair phenotype

(Strutt et al., 2004)

ds¹/ds^UAO71, fj^d1 has posterior crossvein phenotype

(Strutt et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Hair polarity on fj^d1, pk³⁰ double mutant wings is more distal than the pk³⁰ phenotype.

(Hogan et al., 2011)

Adding fj^p1/fj^d1 has little effect on the wing area of ds^UAO71/ds^38k flies.

Adding fj^p1/fj^d1 dramatically reduces the wing area of flies expressing ds^{Act5C.T:Avic\GFP-EGFP} in a ds^UAO71/ds^38k background.

Adding fj^p1/fj^d1 has no effect on the wing area of flies expressing ds^{SAx3.Act5C.T:Avic\GFP-EGFP} in a ds^UAO71/ds^38k background.

Adding fj^p1/fj^d1 has no effect on the wing area of flies expressing ds^{SDx3.Act5C.T:Avic\GFP-EGFP} in a ds^UAO71/ds^38k background.

(Brittle et al., 2010)

The cuticle phenotype of dead embryos derived from baz⁴/+ embryos derived from a cross of baz⁴/+ females to wild-type males is enhanced if the females also carry one copy of fj^d1.

The cuticle phenotype of dead embryos derived from baz^G0484/+ embryos derived from a cross of baz^G0484/+ females to wild-type males is enhanced if the females also carry one copy of fj^d1.

(Shao et al., 2010)

The planar polarity phenotype of ds⁰⁵¹⁴²/ds⁰⁵¹⁴² wings is not enhanced by fj^d1/fj^d1. fj^d1/fj^d1 ds^Scer\UAS.cTa ; Scer\GAL4^αTub84B.PP flies have only mild wing planar polarity defects. The planar polarity defects seen in the wings of ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 flies are midly enhanced by fj^d1/fj^d1 : polarity defects are seen outside the regions of the wing affected by ds^Scer\UAS.cTa; Scer\GAL4^salm-459.2 alone. Planar cell polarity defects in the posterior compartment of ft^Scer\UAS.cMa; Scer\GAL4^en-e16E flies are strongly suppressed by fj^d1/fj^d1.

(Matakatsu and Blair, 2004)

ds^38k fj^N7/ds^UAO71 fj^d1 flies have disorganised ommatidial polarity; no obvious equator is present. Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. The polarity reversals in the eye seen in flies expressing ft^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^bi-omb3-GAL4 is strengthened in a fj^N7/fj^d1 background. Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present.

(Simon, 2004)

fj^d1; ft¹ double homozygotes have a much more severe wing shortening phenotype than either single mutant. The distance between anterior and posterior crossveins in the wings of these flies is reduced to almost zero. The wing shortening phenotype of fj^d1 homozygotes is enhanced by ds^38k/+, ds¹/+ or by ft¹/+. Unlike either single mutant, 'fj^d1 ds¹/fj^d1 ds^UAO71' flies completely lack a posterior crossvein and have defects in wing hair polarity. 16% of ommatidia in these double mutants are inverted compared to less than 1% in either single mutant.

(Strutt et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fj^d1 is rescued by fj^UAS.cZa/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

fj^d1 is rescued by fj^{t14.Tag:Golgi(hGALNT3)}

(Strutt et al., 2004)

Partially rescued by

fj^d1 is partially rescued by fj^+t24

(Strutt et al., 2004)

fj^d1 is partially rescued by fj^{t14.Tag:SS(rCd2)}

(Strutt et al., 2004)

fj^d1 is partially rescued by fj^+t14

(Strutt et al., 2004)

fj^d1 is partially rescued by fj^Un.t14

(Strutt et al., 2004)

Not rescued by

fj^d1 is not rescued by Scer\GAL4^αTub84B.PL/fj^D447Q.UAS

(Brittle et al., 2010)

fj^d1 is not rescued by fj^D454Q.UAS/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

fj^d1 is not rescued by fj^D490Q.UAS/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

Comments

Expression of either fj^{D447Q.Scer\UAS}, fj^{D454Q.Scer\UAS} or fj^{D490Q.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL does not rescue the reversal of hair polarity that is seen behind fj^d1 clones in the dorsal adult abdomen.

(Brittle et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6373

w¹¹¹⁸; fj^d1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fj^D1

(Hogan et al., 2011)

fj^d1

(Brittle et al., 2022, Iijima et al., 2020, Saavedra et al., 2016, Hale et al., 2015, Okumura et al., 2015, Brittle et al., 2012, Matakatsu and Blair, 2012, Brittle et al., 2010, Chung et al., 2009, Feng and Irvine, 2009, Tyler et al., 2007, Casal et al., 2006, Mao et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (26)

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