FlyBase Allele Report: Dmel\Hmgcr[UAS.cvDa]

General Information

Symbol

Dmel\Hmgcr^UAS.cvDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of van Doren

FlyBase ID

FBal0094693

Feature type

allele

Associated gene

Dmel\Hmgcr

Associated Insertion(s)

Carried in Construct

P{UAS-Hmgcr.vD}

Also Known As

UAS-Hmgcr, UAS-clb, UAS hmgcr

Key Links

Transgenic product class

wild_type

(Van Doren et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Van Doren et al., 1998)

Mutagen

in vitro construct

(Van Doren et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-Hmgcr.vD}

Cytology

Description

Expression of the Hmgcr coding region is governed by UASt regulatory sequences.

(Van Doren et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hmgcr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neuronal ceroid lipofuscinosis

modeled by cathD¹

(Kuronen et al., 2009)

exacerbates neurodegenerative disease

modeled by SNF4Aγ^loe

(Tschäpe et al., 2002)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage, with Scer\GAL4^elav.PU

(Barton et al., 2024)

abnormal cell migration | embryonic stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Barton et al., 2024)

abnormal cell migration | embryonic stage | parental effect, with Scer\GAL4^48Y

(Barton et al., 2024)

Phenotype Manifest In

germline cell, with Scer\GAL4^69B

(Van Doren et al., 1998)

germline cell, with Scer\GAL4^elav-C155

(Stein et al., 2002, Van Doren et al., 1998)

germline cell, with Scer\GAL4^h-1J3

(Van Doren et al., 1998)

pole cell & embryonic nervous system, with Scer\GAL4^elav-C155

(Santos and Lehmann, 2004)

primordial germ cell, with Scer\GAL4^elav-C155

(Ricardo and Lehmann, 2009, Ricardo and Lehmann, 2009)

primordial germ cell | embryonic stage 5, with Scer\GAL4^Act.PU

(Jones and Macdonald, 2015)

primordial germ cell | embryonic stage, with Scer\GAL4^elav.PU

(Barton et al., 2024)

primordial germ cell | embryonic stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Barton et al., 2024)

primordial germ cell | embryonic stage | parental effect, with Scer\GAL4^48Y

(Barton et al., 2024)

Detailed Description

Statement

Reference

Germ cells in Hmgcr^{Scer\UAS.cvDa}; Scer\GAL4^elav-C155 embryos migrate ectopically to the CNS.

(Ricardo and Lehmann, 2009, Ricardo and Lehmann, 2009)

In Hmgcr^{Scer\UAS.cvDa}; Scer\GAL4^elav-C155 embryos an avergae of 12 germ cells per embryos migrate abberantly into the nervous system.

(Santos and Lehmann, 2004)

When expression is driven by Scer\GAL4^h-1J3, some PGCs are found in the gonad though many are lost and accumulate in segment A7. The gonadal mesoderm forms normally. When expression is driven by Scer\GAL4^69B, some PGCs are found in the epidermis. When expression is driven by Scer\GAL4^elav-C155, some PGCs are found in the ventral nerve cord. When expression is driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}, PGC migration is normal. Embryos grow up to be fertile adults.

(Van Doren et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hmgcr^UAS.cvDa, Scer\GAL4^elav.PU has abnormal cell migration | embryonic stage phenotype, suppressible | partially by jhamt^8F1

(Barton et al., 2024)

Hmgcr^UAS.cvDa, Scer\GAL4^48Y has abnormal cell migration | parental effect | embryonic stage phenotype, suppressible | partially by Met¹/gce^2.5k/Met²⁷/gce^MI02742

(Barton et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hmgcr^UAS.cvDa, Scer\GAL4^elav.PU has primordial germ cell | embryonic stage phenotype, suppressible | partially by jhamt^8F1

(Barton et al., 2024)

Hmgcr^UAS.cvDa, Scer\GAL4^48Y has primordial germ cell | parental effect | embryonic stage phenotype, suppressible | partially by Met¹/gce^2.5k/Met²⁷/gce^MI02742

(Barton et al., 2024)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, suppressible | partially by +/Df(2R)Exel7123

(Ricardo and Lehmann, 2009, Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, suppressible | partially by Mdr49[+]/Mdr49^KG08611

(Ricardo and Lehmann, 2009, Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, suppressible | partially by Df(3L)ED4486/+

(Ricardo and Lehmann, 2009)

NOT suppressed by

Statement

Reference

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, non-suppressible by Df(2R)Exel6105/+

(Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, non-suppressible by Df(3L)Exel6108/+

(Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, non-suppressible by Df(2R)Exel7131/+

(Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has primordial germ cell phenotype, non-suppressible by Df(3L)ED4283/+

(Ricardo and Lehmann, 2009)

Hmgcr^UAS.cvDa, Scer\GAL4^elav-C155 has germline cell phenotype, non-suppressible by slam^waldo1

(Stein et al., 2002)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/Hmgcr^UAS.cvDa is an enhancer of eye phenotype of cathD¹

(Kuronen et al., 2009)

Scer\GAL4^Appl.G1a/Hmgcr^UAS.cvDa is an enhancer of adult brain phenotype of SNF4Aγ^loe

(Tschäpe et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^elav-C155 enhances by more than 10-fold the level of vacuolar change in cathD¹ mutant retina.

(Kuronen et al., 2009)

Ectopic migration of germ cells to the CNS in Hmgcr^{Scer\UAS.cvDa}; Scer\GAL4^elav-C155 embryos is not supppressed by Df(2R)Exel6105/+, Df(3L)Exel6108/+, Df(2R)Exel7131/+, Df(3L)ED4283/+ but is partially suppressed by Mdr49^KG08611/+, Df(2R)Exel7123/+ or Df(2R)Exel6065/+.

(Ricardo and Lehmann, 2009)

Ectopic migration of germ cells to the CNS in Hmgcr^{Scer\UAS.cvDa}; Scer\GAL4^elav-C155 embryos is partially suppressed by Df(3L)ED4486/+. Df(2R)Exel7123/+ or Mdr49^KG08611/+.

(Ricardo and Lehmann, 2009)

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^elav-C155 can attract germ cells in embryos. This phenotype is still seen in a slam^waldo1 mutant background.

(Stein et al., 2002)

The addition of Hmgcr^{Scer\UAS.cvDa} (driven by Scer\GAL4^Appl.G1a) enhances the adult brain degeneration phenotype seen in SNF4Aγ^loe animals.

(Tschäpe et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/Hmgcr^UAS.cvDa rescues Hmgcr⁰⁴⁶⁸⁴

(Belgacem and Martin, 2007)

Scer\GAL4^FRT.Act5C, Scer\GAL4^Hmgcr.B, Hmgcr^UAS.cvDa rescues Hmgcr⁰⁴⁶⁸⁴

(Belgacem and Martin, 2007)

Scer\GAL4^HCH.Hand/Hmgcr^UAS.cvDa rescues Hmgcr⁰¹¹⁵²

(Yi et al., 2006)

Scer\GAL4^Mef2.PR/Hmgcr^UAS.cvDa rescues Hmgcr⁰¹¹⁵²

(Yi et al., 2006)

Partially rescues

Scer\GAL4^FRT.Act5C/Hmgcr^UAS.cvDa partially rescues Hmgcr⁰⁴⁶⁸⁴

(Belgacem and Martin, 2007)

Fails to rescue

Scer\GAL4^elav-C155/Hmgcr^UAS.cvDa fails to rescue Hmgcr⁰⁴⁶⁸⁴

(Belgacem and Martin, 2007)

Scer\GAL4^to.PD/Hmgcr^UAS.cvDa fails to rescue Hmgcr⁰⁴⁶⁸⁴

(Belgacem and Martin, 2007)

Scer\GAL4^Ugt36A1.PK/Hmgcr^UAS.cvDa fails to rescue Hmgcr⁰¹¹⁵²

(Yi et al., 2006)

Comments

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^to.PD fails to rescue the lethality found in Hmgcr⁰⁴⁶⁸⁴ homozygotes.

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^{Scer\FRT.Act5C} completely rescues lethality, but does not rescue the sexual dimorphism.

Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^da.G32 is sufficient to rescue both the lethality (partly in males - 75%, and completely in females) and the start/stop number (indicating locomotor activity).

Targeted expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^{Scer\FRT.Act5C} and Scer\GAL4^Hmgcr.B is sufficient to rescue the sexual dimorphism.

(Belgacem and Martin, 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hmgcr^{Scer\UAS.cvDa}

Hmgcr^UAS.cvDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of van Doren

(Van Doren et al., 1998)

Secondary FlyBase IDs

References (18)

Report Sections

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