FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Droncpro.UAS
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General Information
Symbol
Dmel\Droncpro.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0104745
Feature type
allele
Associated gene
Dmel\Dronc
Associated Insertion(s)
Carried in Construct
P{UAS-pro-dronc}
Also Known As
UAS-dronc, UAS-pro-dronc, UAS-proDronc, UAS-droncWT
Key Links
Transgenic product class
wild_type
(Meier, 2000.4.5)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Meier, 2000.4.5)
Mutagen
in vitro construct
(Meier, 2000.4.5)
Progenitor genotype
Carried in construct
P{UAS-pro-dronc}
Cytology
Description

The complete coding sequence of Dronc from nucleotides 369 to 1721 is expressed under the control of UASt sequences.

(Meier, 2000.4.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Dronc
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF results in pigment cell death.

      (Domingues and Ryoo, 2012)

      Overexpression of Ncpro.Scer\UAS in the eye-antennal disc under the control of Scer\GAL4GMR.PF induces cell death. The adult eyes appear normal-sized, and show a 'spotty-pigmented-eye' phenotype.

      Overexpression of Ncpro.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF does not affect cell division but slightly increases apoptosis compared to wild-type.

      (Verghese et al., 2012)

      Expression of Ncpro.Scer\UAS under the control Scer\GAL4c42 results in semi-lethality during larval and pupal development. The morphology of larval Malpighian tubules is disrupted in these animals. Tubules show reduced size, number, and some segments are missing. Other tubules appear to be reduced in length.

      (Shukla and Tapadia, 2011)

      Scer\GAL4GMR.PF-mediated expression of a weak Ncpro.Scer\UAS results in adult eyes with normal external morphology but with internal ablation of most photoreceptor and pigment cells. Eyes show only very small patches of red pigmentation. Expression of a strong Ncpro.Scer\UAS results in more severely malformed and depigmented eyes.

      At 25[o]C, Scer\GAL4GMR.PF-mediated expression of the strong NcDeltaN.Scer\UAS fail to eclose out of the pupal case. At 18[o]C, a few flies emerge with completely ablated eyes and reduced head size.

      (Mallik and Lakhotia, 2009)

      Flies carrying a weakly expressed Ncpro.Scer\UAS line driven by Scer\GAL4GMR.PF show near regular arrays of ommatidia, but show uneven pigmentation of the eye. No pseudopupil image is found in these flies.

      Flies carrying a strongly expressed Ncpro.Scer\UAS line driven by Scer\GAL4GMR.PF have severely degenerated and depigmented eyes with reduced head size. The majority of these animals die as pharate adults.

      (Arya and Lakhotia, 2008)

      Expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PU induces apoptosis in the third larval instar eye disc.

      (Xu et al., 2006)

      Expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF results in loss of pigment cells in the eye.

      (Ryoo et al., 2002)

      When driven by Scer\GAL4GMR.PF Jafrac2AKP.Ub.GMR causes a 'spotted eye' phenotype, exhibiting occasional red spots, despite being in a w- background.

      (Tenev et al., 2002)

      When expression of Ncpro.Scer\UAS is driven by Scer\GAL4GMR.PF, A "spotted eye" phenotype is seen in the weak insertion P{UAS-pro-dronc}W. The eyes are mostly white, but with occasional red spots. External morphology of the eye is normal, however internally the pigment cells and photoreceptors are abnormal: Only remnants of pigment cells and vacuole like structures remain. There is a dramatic increase in numbers of apoptotic cells in the eye discs of pupae. The remnant pigment cells cause the red spot phenotype. In the stronger insertion P{UAS-pro-dronc}S flies exhibit roughened eyes that are reduced in size. Their surface morphology is distorted, disrupted and rough. The eyes are white, and the internal morphology of photoreceptors is completely disrupted. An increase in apoptosis is seen in larval eye discs. The addition of thGMR.PH to Ncpro.Scer\UAS,Scer\GAL4GMR.PF flies completely rescues the phenotypes seen in these flies. The addition of Df(3L)th102 to Ncpro.Scer\UAS,Scer\GAL4GMR.PF flies produces flies that display severely distorted eyes and die trapped in their pupal cases. The addition of BacA\p35GMR.PH to Ncpro.Scer\UAS,Scer\GAL4GMR.PF weakly suppresses the phenotypes seen in those flies, leading to a slightly increased eye size.

      (Meier et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Droncpro.UAS, Scer\GAL4GMR.PU has pigment cell phenotype, non-suppressible by Bruce[+]/BruceE23

      (Domingues and Ryoo, 2012)

      Droncpro.UAS, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Bruce[+]/BruceE23

      (Domingues and Ryoo, 2012)

      DarkUAS.Tag:polyHis,Tag:MYC, Droncpro.UAS, Scer\GAL4GMR.PU has pigment cell phenotype, non-suppressible by BruceE81/Bruce[+]

      (Domingues and Ryoo, 2012)

      DarkUAS.Tag:polyHis,Tag:MYC, Droncpro.UAS, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by BruceE81/Bruce[+]

      (Domingues and Ryoo, 2012)

      DarkUAS.Tag:polyHis,Tag:MYC, Droncpro.UAS, Scer\GAL4GMR.PU has pigment cell phenotype, non-suppressible by Bruce[+]/BruceE23

      (Domingues and Ryoo, 2012)

      DarkUAS.Tag:polyHis,Tag:MYC, Droncpro.UAS, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Bruce[+]/BruceE23

      (Domingues and Ryoo, 2012)

      Droncpro.UAS, Scer\GAL4GMR.PU has pigment cell phenotype, non-suppressible by BruceE81/Bruce[+]

      (Domingues and Ryoo, 2012)

      Droncpro.UAS, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by BruceE81/Bruce[+]

      (Domingues and Ryoo, 2012)

      Droncpro.UAS, Scer\GAL4GMR.PF has eye phenotype, non-suppressible by Dcp-1RNAi.UAS.cLa, Scer\GAL4GMR.PF

      (Leulier et al., 2006)

      Droncpro.UAS has eye phenotype, non-suppressible by Scer\GAL4GMR.PF/StricaRNAi.UAS.cLa

      (Leulier et al., 2006)

      Droncpro.UAS, Scer\GAL4GMR.PU has eye disc | third instar larval stage phenotype, non-suppressible by Drice17/Drice17

      (Xu et al., 2006)

      Droncpro.UAS, Scer\GAL4GMR.PF has pigment cell phenotype, non-suppressible by BacA\p35GMR.PH

      (Meier et al., 2000)

      Droncpro.UAS, Scer\GAL4GMR.PF has ommatidium phenotype, non-suppressible by BacA\p35GMR.PH

      (Meier et al., 2000)
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos co-expressing Droncpro.Scer\UAS and DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in neurons under the control of Scer\GAL4elav.PU exhibit increased engulfment of apoptotic neurons.

      (Shklyar et al., 2013)

      The combined expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF generates a potent eye ablation phenotype.

      A BruceE81 or BruceE23 heterozygous background does not affect the pigment cell death phenotype caused by the overexpression of Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF.

      Either a BruceE81/+ or BruceE23/+ heterozygous background has no effect on the eye ablation phenotype found upon co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF.

      (Domingues and Ryoo, 2012)

      Co-expression of Ncpro.Scer\UAS in ykiScer\UAS.cHa-expressing cells (both under the control of Scer\GAL4GMR.PF) results in a decrease in cell division and an increase in apoptosis, compared to expression of ykiScer\UAS.cHa alone.

      Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS together, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal discs and adult eyes.

      Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC, Ncpro.Scer\UAS and hpoScer\UAS.cUa in the developing eye, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal disc and adult. This phenotype appears stronger than controls as both photoreceptor and pigment cells are eventually eliminated in animals co-expressing hpoScer\UAS.cUa.

      (Verghese et al., 2012)

      Expression of Ncpro.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation.

      (Ouyang et al., 2011)

      Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in flies expressing either the weak or strong Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF.

      Co-expression of P{Sym-UAS-Hsrω} rescues the pupal lethality and small head phenotypes seen in flies expressing the strong Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF.

      (Mallik and Lakhotia, 2009)

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS suppresses the eye phenotypes caused by both weakly and strongly expressing Ncpro.Scer\UAS lines driven by Scer\GAL4GMR.PF, and all these animals eclose with normal head size.

      (Arya and Lakhotia, 2008)

      Homozygosity for Ice17 does not suppress the apoptosis induced in the third larval instar eye disc by expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PU.

      (Xu et al., 2006)

      The loss of pigment cells in the eye caused by expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by effD73/+.

      (Ryoo et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Droncpro.UAS is rescued by Scer\GAL4GMR.PF/DroncRNAi.UAS.cLa

      (Leulier et al., 2006)
      Rescues

      Droncpro.UAS rescues DroncI29/DroncL32

      (Napoletano et al., 2017)
      Comments

      The expression of Droncpro.Scer\UAS under the control of Scer\GAL4nos.PU rescues both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of testes observed in DroncI29/DroncL32 transheterozygotes.

      (Napoletano et al., 2017)

      Expression of NcdsRNA.Scer\UAS.cLa in animals expressing Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF rescues the eye phenotype observed when Ncpro.Scer\UAS is expressed alone.

      (Leulier et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Droncpro.Scer\UAS
      Droncpro.UAS
      Ncpro.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (22)