FlyBase Allele Report: Dmel\Src64B[UY1332]

General Information

Symbol

Dmel\Src64B^UY1332

Species

D. melanogaster

Name

FlyBase ID

FBal0155788

Feature type

allele

Associated gene

Dmel\Src64B

Associated Insertion(s)

P{Mae-UAS.6.11}Src64B^UY1332

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Nicolai et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{Mae-UAS.6.11}Src64B^UY1332

Cytology

Description

Insertion site maps to +1.5kb of the known/supposed transcription start site. The insertion is upstream of at least one of the known translation start sites.

(Nicolai et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{Mae-UAS.6.11}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA

(Pagarigan et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of carcinoma

is ameliorated by PRL-1^UAS.cPa

(Pagarigan et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

adult mushroom body alpha-lobe, with Scer\GAL4^7B

(Nicolai et al., 2003)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

adult mushroom body beta-lobe, with Scer\GAL4^7B

(Nicolai et al., 2003)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

epithelial cell, with Scer\GAL4^hh-Gal4

(Fernández et al., 2014)

Kenyon cell, with Scer\GAL4^7B

(Nicolai et al., 2003)

Kenyon cell, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

mushroom body & axon, with Scer\GAL4^7B

(Nicolai et al., 2003)

mushroom body & axon, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

wing blade, with Scer\GAL4^hh-Gal4

(Fernández et al., 2014)

wing disc, with Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

wing disc | larval stage, with Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

Detailed Description

Statement

Reference

Expression of Src64B^UY1332 in the posterior compartment of the wing imaginal disc, under the control of Scer\GAL4^hh-Gal4 results in epithelial extrusion of cells moving to distant areas from the posterior compartment and activation of the apoptosome component Nc (and a subsequent increase in apoptosis).

Expression of Src64B^UY1332 under Scer\GAL4^sd-SG29.1 control at 25[o]C induces massive apoptosis. At 18[o]C, these tissues do not contain apoptotic cells.

Expression of Src64B^UY1332 under the control of Scer\GAL4^hh-Gal4 decreases the size of the posterior imaginal wing disc blade domain, relative to the total blade area, to 19.4% from 34.6% in controls.

(Fernández et al., 2014)

Expression of Src64B^UY1332 under the control of Scer\GAL4^ap-md544 results in pupal lethality.

Larval wing discs expressing Src64B^UY1332 in the dorsal compartment of the wing disc under the control of Scer\GAL4^ap-md544 are grossly overgrown and exhibit elevated levels of apoptosis, extending beyond the dorsal compartment.

(Pagarigan et al., 2013)

When expression is driven by Scer\GAL4^7B, Src64B^UY1332 shows a mushroom body Class III phenotype; axonal growth and guidance defects. The beta lobes are abortive and the alpha lobes enlarged, particularly at the basal end. Phenotype is similar though more dramatic - all axons stop at the peduncle - when Src64B^UY1332 is driven by Scer\GAL4^OK107.

(Nicolai et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub-AC-62, Src64B^UY1332 has increased cell death phenotype, enhanceable by cpa^RNAi.UAS.cFa/Scer\GAL4^nub-AC-62

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell death | heat sensitive phenotype, enhanceable by Scer\GAL4^sd-SG29.1/cpb^UAS.cWa/cpa^RNAi.UAS.cFa

(Fernández et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src64B^UY1332 has increased occurrence of cell division | adult stage phenotype, suppressible by Stat92E^RNAi.UAS.cUa/Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell death | heat sensitive phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell death | heat sensitive phenotype, suppressible by shg[+]/shg^k03401

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell death | heat sensitive phenotype, suppressible by Btk29A[+]/Btk^k00206

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell death | heat sensitive phenotype, suppressible by p120ctn[+]/p120ctn³⁰⁸

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has decreased cell growth rate | heat sensitive phenotype, suppressible by shg[+]/shg^k03401

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has decreased cell growth rate | heat sensitive phenotype, suppressible by Btk29A[+]/Btk^k00206

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has decreased cell growth rate | heat sensitive phenotype, suppressible by p120ctn[+]/p120ctn³⁰⁸

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has decreased cell growth rate | heat sensitive phenotype, suppressible by Rho1^1B/Rho1[+]

(Fernández et al., 2014)

BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell growth rate phenotype, suppressible by bsk^DN.UAS, Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell growth rate phenotype, suppressible by yki^NIG.4005R, Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1, Src64B^UY1332 has increased cell growth rate phenotype, suppressible by cpb^UAS.cWa/cpa^UAS.N.Tag:HA, Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

Scer\GAL4^ap-md544, Src64B^UY1332 has lethal - all die before end of pupal stage phenotype, suppressible | partially by PRL-1^UAS.cPa, Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

Scer\GAL4^ap-md544, Src64B^UY1332 has hyperplasia | larval stage phenotype, suppressible | partially by PRL-1^UAS.cPa, Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

Scer\GAL4^ap-md544, Src64B^UY1332 has increased cell death | larval stage phenotype, suppressible | partially by PRL-1^UAS.cPa, Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

Scer\GAL4^ap-md544, Src64B^UY1332 has lethal - all die before end of pupal stage phenotype, suppressible | partially by PRL-1^UAS.NC, Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src64B^UY1332 has increased occurrence of cell division | adult stage phenotype, non-suppressible by dome^ΔCYT.UAS/Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^NP7397, Src64B^UY1332 has increased occurrence of cell division | adult stage phenotype, non-suppressible by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^C57, Src64B^UY1332 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

PRL-1^UAS.NC, Scer\GAL4^ap-md544, Src64B^UY1332 has partially lethal - majority die phenotype

(Pagarigan et al., 2013)

PRL-1^UAS.NC, Scer\GAL4^ap-md544, Src64B^UY1332 has partially lethal - majority live phenotype

(Pagarigan et al., 2013)

PRL-1^UAS.NC, Scer\GAL4^ap-md544, Src64B^UY1332 has some die during pupal stage phenotype

(Pagarigan et al., 2013)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544, Src64B^UY1332 has some die during pupal stage phenotype

(Pagarigan et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has wing disc phenotype, enhanceable by BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src64B^UY1332 has adult midgut phenotype, suppressible by Stat92E^RNAi.UAS.cUa/Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^hh-Gal4, Src64B^UY1332 has wing blade phenotype, suppressible by shg[+]/shg^k03401

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has wing disc phenotype, suppressible by shg[+]/shg^k03401

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has wing disc phenotype, suppressible by Btk29A[+]/Btk^k00206

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has wing disc phenotype, suppressible by p120ctn[+]/p120ctn³⁰⁸

(Fernández et al., 2014)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has wing disc phenotype, suppressible by Rho1^1B/Rho1[+]

(Fernández et al., 2014)

Scer\GAL4^ap-md544, Src64B^UY1332 has wing disc phenotype, suppressible by PRL-1^UAS.cPa, Scer\GAL4^ap-md544

(Pagarigan et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src64B^UY1332 has adult midgut phenotype, non-suppressible by dome^ΔCYT.UAS/Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^NP7397, Src64B^UY1332 has adult midgut phenotype, non-suppressible by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Scer\GAL4^sd-SG29.1, Src64B^UY1332 has epithelial cell phenotype, non-suppressible by bsk^DN.UAS/Scer\GAL4^sd-SG29.1

(Fernández et al., 2014)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^C57, Src64B^UY1332 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae with expression of Src64B^UY1332 driven in muscles by Scer\GAL4^C57 show significant decreases in mEPSP (decreased quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; when expression is driven neuronally by Scer\GAL4^elav-C155 the significant increase in quantal content is still seen.

(Spring et al., 2016)

Blocking JNK signalling, through expression of dominant-negative bsk^DN.Scer\UAS, significantly abolishes apoptosis and restores growth in imaginal wing discs expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1. However, bsk^DN.Scer\UAS expression does not appear to prevent loss of epithelial integrity as cross-sections through these epithelia show the presence of dividing nuclei on the basal surface.

Co-expression of cpa^{dsRNA.Scer\UAS.cFa} and Src64B^UY1332 under the control of Scer\GAL4^nub-AC-62, results in massive apoptosis.

Co-expression of cpa^{Scer\UAS.N.T:Ivir\HA1} and cpb^Scer\UAS.cWa appears to significantly suppress apoptosis and to restore tissue growth in imaginal wing discs expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1.

Removal of one copy of shg, through a shg^k03401 heterozygous background, restores the size of the posterior imaginal wing disc blade to 28.3% in flies expressing Src64B^UY1332 under the control of Scer\GAL4^hh-Gal4.

Removal of one copy of Btk29A, through a Btk29A^k00206 heterozygous background, significantly abolishes apoptosis and restores tissue growth in flies expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1. However, this does not trigger the presence of mitotic nuclei on the basal cell surface.

Removal of one copy of p120ctn, through a p120ctn³⁰⁸ heterozygous background, significantly abolishes apoptosis and restores tissue growth in flies expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1. However, this does not trigger the presence of mitotic nuclei on the basal cell surface.

Removal of one copy of Rho1, through a Rho1^1B heterozygous background, significantly abolishes apoptosis and restores tissue growth in flies expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1. However, this does not trigger the presence of mitotic nuclei on the basal cell surface.

Apoptotic levels are not significantly different in tissues expressing cpa^{dsRNA.Scer\UAS.cFa} under the control of Scer\GAL4^sd-SG29.1 that are either wild-type or heterozygous for Btk29A^k00206 or p120ctn³⁰⁸. While removing one copy of Rho1^1B enhances apoptosis in cpa-depleted tissues.

(Fernández et al., 2014)

Expression of PRL-1^Scer\UAS.cPa partially suppresses the pupal lethality seen when Src64B^UY1332 is expressed in the dorsal compartment of the wing disc under the control of Scer\GAL4^ap-md544, enabling 45% of the expected adults to eclose. The overgrowth and apoptosis seen in the larval wing discs are also suppressed by PRL-1^Scer\UAS.cPa.

Expression of PRL-1^Scer\UAS.NC partially suppresses the pupal lethality seen when Src64B^UY1332 is expressed under the control of Scer\GAL4^ap-md544, enabling approximately 70% of the expected adults to eclose.

(Pagarigan et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^GMR.PH in wing discs expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1 results in massive tissue overgrowth. Co-expression of bsk^DN.Scer\UAS suppresses the tissue overgrowth seen in these mutants.

Knockdown of yki, through expression of yki^NIG.4005R under the control of Scer\GAL4^sd-SG29.1 prevents Src64B^UY1332 induced tissue growth in BacA\p35^GMR.PH-expressing wing discs.

While expression of BacA\p35^GMR.PH in imaginal wing discs expressing Src64B^UY1332 under the control of Scer\GAL4^sd-SG29.1 increases disc size up to 2.5 times (compared to controls), co-expression of cpa^{Scer\UAS.N.T:Ivir\HA1} and cpb^Scer\UAS.cWa in these tissues significantly reduces tissue growth to 1.8 times that found in controls, but does not affect growth of Scer\GAL4^sd-SG29.1-->BacA\p35^GMR.PH wing discs.

(Fernández et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7342

y¹ w^67c23; P{Mae-UAS.6.11}Src64B^UY1332

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Src64B^UY1332

(Fernández et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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