FlyBase Allele Report: Ecol\lexA[fru.P1]

General Information

Symbol

Ecol\lexA^fru.P1

Species

E. coli

Name

FlyBase ID

FBal0244777

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

TI{lexA::VP16}fru^P1.LexA

Carried in Construct

Also Known As

fru-LexA, fru^LexA, fru^P1.LexA, fruP1.LexA, FruP1-LexA, fru^lex, FruLexA

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Mellert et al., 2010)

I-SceI

(Mellert et al., 2010)

FLPase

(Mellert et al., 2010)

ends-out gene targeting

(Mellert et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{lexA::VP16}fru^P1.LexA

Cytology

Description

Sequence encoding a lexA::VP16 driver has been inserted at the first codon of the fru[M] open reading frame. This allows for expression of the lexA::VP16 driver in the same pattern as transcripts from the fru[M] (P1) promoter, which is transcribed in both males and females.

(Mellert et al., 2010)

Allele components

Component

Use(s)

Inserted element

TI{lexA::VP16}

promoter trap

Encoded product / tool

lexA::VP16

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- adult stage

gustatory receptor neuron | subset of prothoracic tarsal segment

(Mellert et al., 2010)

adult stage

prothoracic leg taste bristle chemosensory neuron | subset

(Lu et al., 2012)

adult brain | restricted

(Li et al., 2011)

adult mushroom body alpha-lobe | restricted

(Li et al., 2011)

adult mushroom body beta-lobe | restricted

(Li et al., 2011)

adult mushroom body gamma-lobe | restricted

(Li et al., 2011)

adult mushroom body alpha'-lobe | restricted

(Li et al., 2011)

adult mushroom body beta'-lobe | restricted

(Li et al., 2011)

antennal lobe glomerulus DA1

(Li et al., 2011)

antennal lobe glomerulus VA1v

(Li et al., 2011)

fruitless expressing neuron

(Wang et al., 2020, Meissner et al., 2016)

fruitless-expressing lobula columnar neuron LC10a

(Ribeiro et al., 2018)

adult doublesex pCd (male) neuron | subset

(Jung et al., 2020)

adult Drosulfakinin MP1 neuron | subset

Comment: co-localizes with P{Dsk-GAL4.W}.

A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with P{Dsk-GAL4.W}. Only stochastic expression observed.

(Wu et al., 2019)

adult Drosulfakinin MP3 neuron | subset

Comment: co-localizes with P{Dsk-GAL4.W}.

A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with P{Dsk-GAL4.W}. Only stochastic expression observed.

(Wu et al., 2019)

adult fruitless P1 (male) neuron

Comment: A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with TI{GAL4::p65}CCKLR-17D3^GAL4::p65.

(Wu et al., 2019)

oviposition descending neuron oviDN

(Wang et al., 2020)

labellar taste bristle mechanosensory neuron | subset

(Thistle et al., 2012)

leg taste bristle chemosensory neuron | subset

(Thistle et al., 2012)

adult fruitless pIP10 (male) neuron

(Lillvis et al., 2024)

adult fruitless pMP2 neuron

(Lillvis et al., 2024)

adult fruitless dPR1 neuron

(Lillvis et al., 2024)

adult fruitless dMS9 neuron

(Lillvis et al., 2024)

adult fruitless vMS12 neuron

(Lillvis et al., 2024)

adult doublesex TN1A (male) neuron

(Lillvis et al., 2024)

adult fruitless dMS2 neuron

(Lillvis et al., 2024)

adult fruitless vPR9 neuron

(Lillvis et al., 2024)

adult fruitless dMS5 neuron

(Lillvis et al., 2024)

adult fruitless dMS10 neuron

(Lillvis et al., 2024)

adult doublesex TN1C (male) neuron

(Lillvis et al., 2024)

adult fruitless vMS13 neuron

(Lillvis et al., 2024)

adult fruitless vMS16 neuron

(Lillvis et al., 2024)

adult fruitless vMS17 neuron

(Lillvis et al., 2024)

adult fruitless vPR1 neuron

(Lillvis et al., 2024)

adult fruitless vPR6 (male) neuron

(Lillvis et al., 2024)

adult fruitless vPR13 neuron

(Lillvis et al., 2024)

descending neuron of the posterior brain DNp13 (male)

(Lillvis et al., 2024)

adult doublesex TN1B (male) neuron

(Lillvis et al., 2024)

adult doublesex TN1D (male) neuron

(Lillvis et al., 2024)

adult doublesex TN1E (male) neuron

(Lillvis et al., 2024)

adult fruitless vMS11 neuron

(Lillvis et al., 2024)

adult fruitless vPR7 neuron

(Lillvis et al., 2024)

descending neuron of the posterior brain DNp36 (male)

(Lillvis et al., 2024)

adult stage | female

neuron | subset of lateral oviduct

Comment: 1 neuron

(Rezaval et al., 2012)

neuron | subset of uterus

Comment: 3 neurons

(Rezaval et al., 2012)

adult post-embryonic ventral posterior Ilp7 (female) neuron | subset

(Feng et al., 2014)

adult stage | male

adult doublesex pC1 (male) neuron | subset

(Zhou et al., 2014)

adult doublesex pC2 (male) neuron | subset

(Zhou et al., 2014)

adult doublesex pCd (male) neuron | subset

(Zhou et al., 2014)

Additional Information

Statement

Reference

Drives expression in most fruitless expressing neurons in the adult, including neurons of the male and female reproductive systems, and a small subset of gustatory receptor neurons.

(FlyBase Curators, 2017)

Ecol\lexA^fru.P1 drives expression in mechanosensory neurons in the taste bristles of the proboscis. In the legs, Ecol\lexA^fru.P1 is detected in more then one gustatory receptor neuron per bristle, with these cells also expressing Scer\GAL4^ppk23.2.695. Ecol\lexA^fru.P1 positive neurons do not overlap with expression of Scer\GAL4^Gr64f.9.7 which labels the sugar-responsive neurons, candidate pheromone receptors Scer\GAL4^Gr32a.3.776 and Scer\GAL4^Gr68a.PB, or with Scer\GAL4^Gr66a.3.153 which labels bitter-responsive neurons.

(Thistle et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in chemosensory neurons in the male forelegs. Most of these chemosensory neurons labeled with EcollexA^{fru.P1.T:HsimVP16} also express ScerGAL4^ppk23.2.6.

(Lu et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in mechanosensory neurons in the taste bristles of the proboscis. In the legs, EcollexA^{fru.P1.T:HsimVP16} is detected in more then one gustatory receptor neuron per bristle, with these cells also expressing ScerGAL4^ppk23.2.695. EcollexA^{fru.P1.T:HsimVP16} positive neurons do not overlap with expression of ScerGAL4^Gr64f.9.7 which labels the sugar-responsive neurons, candidate pheromone receptors ScerGAL4^Gr32a.3.776 and ScerGAL4^Gr68a.PB, or with ScerGAL4^Gr66a.3.153 which labels bitter-responsive neurons.

(Thistle et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in 2 clusters of neurons in the adult female genital tract, which co-express AvicGFP^1.E.ppk : one cluster of 1 neuron in the lateral oviduct and one cluster of 3 neurons in the uterus (which also express ScerGAL4^dsx.KI).

(Rezaval et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in many neurons in the adult brain, including in the mushroom body alpha, beta, gamma, alpha', beta' lobes. In the antennal lobe, EcollexA^{fru.P1.T:HsimVP16} labels the antennal lobe glomeruli DA1 and VA1v.

(Li et al., 2011)

EcollexA^fru.P1 drives expression in in a subset of gustatory neurons of prothoracic tarsal segments 3-5 at 18-20 hours APF; no expression is observed prothoracic tarsal segments 1-2 at this stage. By 24-28 hours, expression is driven in gustatory neurons of all prothoracic tarsal segments. At the earlier timepoint, expression is observed in both male and female prothoracic tarsi, while expression at 28 hours APF is much more pronounced in male than female tarsi.

(Mellert et al., 2010)

Marker for

fruitless expressing neuron

(Wang et al., 2020)

Reflects expression of

fru

(Wang et al., 2020, Wang et al., 2020, Ribeiro et al., 2018, Meissner et al., 2016, Zhou et al., 2014, Lu et al., 2012, Rezaval et al., 2012, Thistle et al., 2012, Li et al., 2011, Mellert et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP.cDa, Scer\GAL4^Tk

(Asahina et al., 2014)

abnormal behavior | adult stage | heat sensitive, with Scer\GAL4^Tk, TrpA1^UAS.mCherry

(Asahina et al., 2014)

abnormal behavior | adult stage | heat sensitive, with Scer\GAL4^Tk, TrpA1^UAS.Tag:MYC

(Asahina et al., 2014)

abnormal courtship behavior, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR43D01

(Kallman et al., 2015)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR21B12

(Zhou et al., 2015)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR22B11

(Zhou et al., 2015)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR46F09

(Zhou et al., 2015)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR49F09

(Zhou et al., 2015)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR72E10

(Zhou et al., 2015)

abnormal courtship behavior | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR46F09

(Zhou et al., 2015)

abnormal courtship behavior | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR72E10

(Zhou et al., 2015)

abnormal courtship behavior | male limited | heat sensitive, with Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR43D01, TrpA1^{FRT.stop.UAS.Tag:MYC}

(Kallman et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\TeTxLC^FRT.stop.UAS with Scer\FLP1^L.8xlexAop) of neurons expressing Ecol\lexA^fru.P1 and any of Scer\GAL4^GMR21B12, Scer\GAL4^GMR22B11, Scer\GAL4^GMR49F09, Scer\GAL4^GMR72E10, or Scer\GAL4^GMR46F09 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.20xUAS.FRT.Venus} with Scer\FLP1^L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexA^fru.P1 and any of Scer\GAL4^GMR72E10, Scer\GAL4^GMR46F09, but not Scer\GAL4^GMR22B11 or Scer\GAL4^GMR49F09, significantly increases male-male courtship chaining, compared to controls.

(Zhou et al., 2015)

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) increases male-male aggression compared to controls. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from TrpA1^{Scer\FRT.stop.Scer\UAS.T:Disc\RFP-mCherry} enabling these cells to express TrpA1 under the control of Scer\GAL4^Tk when the flies are shifted to the permissive temperature. Activation of Tk-GAL4[Fru[M] neurons does not induce aggression of male flies towards females, and they copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged.

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) increases male-male aggression compared to controls. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from TrpA1^{Scer\FRT.stop.Scer\UAS.T:Hsap\MYC} enabling these cells to express TrpA1 under the control of Scer\GAL4^Tk when the flies are shifted to the permissive temperature. There is no change in male-male courtship behavior; the frequency of unilateral wing extension or "singing" towards other males is unchanged.

Silencing of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) reduces levels of male-male aggression compared to controls, although the level of baseline aggression between different controls varies. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from Hsap\KCNJ2^{Scer\FRT.Stop.Scer\UAS.T:Avic\GFP-EGFP} enabling these cells to express Hsap\KCNJ2 under the control of Scer\GAL4^Tk. The males copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged. Silencing of Tk-GAL4[Fru[M] neurons does not consistently influence the frequency of unilateral wing extensions towards other males in comparison with control strains.

(Asahina et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Ecol\lexA^{fru.P1.T:Hsim\VP16}

Ecol\lexA^fru.P1

Fru^P1.LexA

(Deutsch et al., 2020)

fru-LEXA

(Koh et al., 2014)

fru-LexA

(Liu et al., 2012, Starostina et al., 2012)

fru-P1-LexA

(Meissner et al., 2011)

fru^LexA

(Zhao et al., 2024, Chen et al., 2021, Wu et al., 2019, Ribeiro et al., 2018, Aranha et al., 2017, Meissner et al., 2016, Yilmazer et al., 2016, Zhou et al., 2015, Vaughan et al., 2014, Zhou et al., 2014)

fru^P1.LexA

(Wohl et al., 2020, Asahina et al., 2014, Zhou et al., 2014, Mellert et al., 2010, Robinett et al., 2010)

fru^P1LexA

(Rezaval et al., 2012)

fru^lex

(Devineni et al., 2013, Fan et al., 2013, Fan et al., 2013)

fru^lexA

(Toda et al., 2012)

fruP1.LexA

(Lu et al., 2012)

fruP1.lexA

(Li et al., 2011)

fruP1LexA

(Li et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (56)

Report Sections

Open Close