FlyBase Allele Report: Dmel\tra[KO.Cherry]

General Information

Symbol

Dmel\tra^KO.Cherry

Species

D. melanogaster

Name

FlyBase ID

FBal0325667

Feature type

allele

Associated gene

Dmel\tra

Associated Insertion(s)

TI{mCherry}tra^KO.Cherry

Carried in Construct

Also Known As

tra^KO

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Hudry et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{mCherry}tra^KO.Cherry

Cytology

Description

A deletion has removed 342 nucleotides, starting 13 nucleotides upstream of the transcription start site of tra; the sequence that replaces the deleted region includes mCherry.

(Hudry et al., 2016)

Allele components

Component

Use(s)

Inserted element

TI{mCherry}

promoter trap

Encoded product / tool

mCherry

red fluorescent protein

Tagged with

mCherry

red fluorescent protein

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:16,590,722..16,591,063 [-]

Comment:

A 342 deletion starting 13bp upstream of the tra transcription start site. The deleted region has been replaced with a sequence which includes mCherry.

(Hudry et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sex-determination | female (with tra^P-Gal4)

(Hérault et al., 2024)

abnormal sex-determination | female (with tra^ΔRS)

(Hérault et al., 2024)

abnormal sex-determination | female | recessive

(Hérault et al., 2024)

abnormal size | adult stage (with tra^F.K-IN)

(Hérault et al., 2024)

abnormal size | adult stage | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

atrophy | adult stage | female | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

decreased body weight | adult stage | female (with tra^ΔRS)

(Hérault et al., 2024)

decreased body weight | female | recessive

(Hérault et al., 2024)

decreased body weight | female | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

decreased size | adult stage | female, with Scer\GAL4^{tra-F-KO-Gal4}, tra^ΔRS.UAS.F

(Hérault et al., 2024)

decreased size | adult stage | female (with tra^ΔRS)

(Hérault et al., 2024)

decreased size | adult stage | female | recessive

(Hérault et al., 2024)

female semi-sterile (with tra^KO.Dvir-tra)

(Hérault et al., 2024)

female sterile | adult stage | female (with tra^ΔRS)

(Hérault et al., 2024)

female sterile | recessive

(Hérault et al., 2024, Miguel-Aliaga, 2017.2.22)

female sterile | somatic clone - tissue specific (with tra^{Dmel.FRT.Dvir})

(Hérault et al., 2024)

female sterile | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

increased body weight | adult stage | male (with tra^F.K-IN)

(Hérault et al., 2024)

male fertile

(Miguel-Aliaga, 2017.2.22)

male sterile (with tra^F.K-IN)

(Hérault et al., 2024)

visible | adult stage (with tra^F.K-IN)

(Hérault et al., 2024)

visible | adult stage (with tra^P-Gal4)

(Hérault et al., 2024)

visible | adult stage | female, with Scer\GAL4^{tra-F-KO-Gal4}, tra^ΔRS.UAS.F

(Hérault et al., 2024)

visible | adult stage | female (with tra^ΔRS)

(Hérault et al., 2024)

visible | adult stage | female | recessive

(Hérault et al., 2024)

visible | adult stage | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

Phenotype Manifest In

ovary | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

wing (with tra^F.K-IN)

(Hérault et al., 2024)

wing | female

(Hérault et al., 2024)

wing | female, with Scer\GAL4^{tra-F-KO-Gal4}, tra^ΔRS.UAS.F

(Hérault et al., 2024)

wing | female (with tra^ΔRS)

(Hérault et al., 2024)

wing | somatic clone - tissue specific (with tra^KO.FRT.traF)

(Hérault et al., 2024)

Detailed Description

Statement

Reference

Under control conditions, tra^KO.Cherry/tra¹ transheterozygotes and tra^KO.Cherry/tra¹ transheterozygotes that also express tra^UAS.cFa under the control of Scer\GAL4^esg-NP7397 during adulthood do not show significant changes in the adult posterior midgut mitotic index in females, as compared to controls.

(Hudry et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tra^KO.Cherry has decreased size | recessive | female | adult stage phenotype, suppressible by Dsec\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has abnormal sex-determination | recessive | female phenotype, suppressible by Dsec\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has female sterile | recessive phenotype, suppressible by Dsec\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has decreased size | recessive | female | adult stage phenotype, suppressible by Dvir\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has abnormal sex-determination | recessive | female phenotype, suppressible by Dvir\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has visible | recessive | female | adult stage phenotype, suppressible by Dsec\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has visible | recessive | female | adult stage phenotype, suppressible by Dvir\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has abnormal sex-determination | recessive | female phenotype, suppressible by Dpse\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has decreased size | recessive | female | adult stage phenotype, suppressible by Dpse\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has visible | recessive | female | adult stage phenotype, suppressible by Dpse\tra^tra.WT

(Hérault et al., 2024)

NOT suppressed by

Statement

Reference

tra^KO.Cherry has decreased size | recessive | female | adult stage phenotype, non-suppressible by Tcas\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has female sterile | recessive phenotype, non-suppressible by Tcas\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has abnormal sex-determination | recessive | female phenotype, non-suppressible by Tcas\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has visible | recessive | female | adult stage phenotype, non-suppressible by Tcas\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has female sterile | recessive phenotype, non-suppressible by Dpse\tra^tra.WT

(Hérault et al., 2024)

Suppressor of

Statement

Reference

tra^KO.Cherry/Df(3L)st-j7 is a suppressor of increased occurrence of cell division | female limited | adult stage phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

Other

Statement

Reference

dsx^Fcons/dsx¹, tra^KO.Dvir-tra/tra^KO.Cherry has female semi-sterile phenotype

(Hérault et al., 2024)

Dvir\tra^tra.WT, tra^KO.Cherry has visible | female | adult stage phenotype

(Hérault et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

tra^KO.Cherry has wing | female phenotype, suppressible by Dpse\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has wing | female phenotype, suppressible by Dsec\tra^tra.WT

(Hérault et al., 2024)

tra^KO.Cherry has wing | female phenotype, suppressible by Dvir\tra^tra.WT

(Hérault et al., 2024)

NOT suppressed by

Statement

Reference

tra^KO.Cherry has wing | female phenotype, non-suppressible by Tcas\tra^tra.WT

(Hérault et al., 2024)

Suppressor of

Statement

Reference

tra^KO.Cherry/Df(3L)st-j7 is a suppressor of adult posterior midgut epithelium | female limited phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

Other

Statement

Reference

Dvir\tra^tra.WT, tra^KO.Cherry has adult abdomen | female phenotype

(Hérault et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

tra^KO.Cherry/Df(3L)st-j7 transheterozygosity suppresses the increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of N^KK102890 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression).

(Hudry et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67412

w¹¹¹⁸/Dp(1;Y)Bar^S; TI{mCherry}tra^KO.Cherry/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tra^KO.Cherry

tra^KO;Cherry

tra^KO

(Hérault et al., 2024, Wat et al., 2021, Hudry et al., 2019, Garner et al., 2018, Hudry et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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