FlyBase Gene Report: Dmel\CCT1

General Information

Symbol

Dmel\CCT1

Species

D. melanogaster

Name

Chaperonin containing TCP1 subunit 1

Annotation Symbol

CG5374

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003676

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. (UniProt, P12613)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

T-cp1, Tcp1-like, Tcp1, T-cpl, TCP1α

Key Links

Genomic Location

Cytogenetic map

94B1-94B1

Sequence location

3R:22,489,105..22,491,439 [-]

Recombination map

3-75

RefSeq locus

NT_033777 REGION:22489105..22491439

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR002423, InterPro:IPR012715

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR012715

(InterPro Project Members, 2004-)

enables ATP-dependent protein folding chaperone

inferred from electronic annotation with InterPro:IPR017998

(InterPro Project Members, 2004-)

enables unfolded protein binding

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR012715

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN004253040

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Jia et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in protein folding

inferred from biological aspect of ancestor with PANTHER:PTN004253040

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR012715

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of chaperonin-containing T-complex

inferred from biological aspect of ancestor with PANTHER:PTN000144020

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CHAPERONIN CONTAINING TCP-1 COMPLEX

Protein Family (UniProt)

Belongs to the TCP-1 chaperonin family. (P12613)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Group (FlyBase)

CHAPERONIN CONTAINING TCP-1 COMPLEX -: Group II Heat Shock Protein 60 chaperonins are paralogous subunits that assemble to form a multi-subunit ring complex, the TCP-1 Ring Complex (TRiC) or Chaperonin Containing TCP-1 (CCT) complex. Within the cytoplasm, TRiC/CCT catalyses the ATP-dependent folding of approximately 10% of of newly synthesized proteins. (Adapted from FBrf0210519, FBrf0232269 and PMID:11580267).

Protein Function (UniProtKB)

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.

(UniProt, P12613)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

T-cp1: T-complex 1

Codes for a cDNA from Drosophila melanogaster that shares 66% sequence identity with the mouse Tcp-1 gene encoding TCP1 (t-complex polypeptide 1).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CCT1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P12613)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CCT1-RA

FBtr0084291

NM_079726

1980

557

CCT1-RB

FBtr0084290

NM_170016

1916

557

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CCT1-PA

FBpp0083684

59.6

557

6.30

FBpp0083683

59.6

557

6.30

P12613

NP_732748

AAN13906

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

557 aa isoforms: CCT1-PA, CCT1-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

(UniProt, P12613)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CCT1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.37

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

trunk visceral muscle primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

Expression Deduced from Reporters

Reporter: PBac{602.P.SVS-1}CCT1^CPTI000037

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CCT1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CCT1

View exonic expression by tissue for Dmel\CCT1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003676

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42649/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003676

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003676

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003676

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5374

Flygut - An atlas of the Drosophila adult midgut

FBgn0003676

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CCT1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CCT1

Transgenic constructs containing regulatory region of CCT1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6191

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CCT1^GD10468

lethal, with Scer\GAL4^tin.CΔ4

CCT1^GD10468

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CCT1^GD10468

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CCT1^GD10468

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

CCT1^GD10468

lethal - all die during pharate adult stage, with Scer\GAL4^ey.PU

Other Phenotypes

Allele

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

CCT1^HMS00639

some die during pupal stage, with Scer\GAL4^pnr-MD237

CCT1^GD10468

visible | adult stage, with Scer\GAL4^eya.enh1+E+2

CCT1^HMS00639

visible | pharate adult stage, with Scer\GAL4^ey.PU

Phenotype manifest in

Allele

adult head | pharate adult stage, with Scer\GAL4^ey.PU

adult heart

CCT1^c03987

eye, with Scer\GAL4^eya.enh1+E+2

CCT1^HMS00639

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\TCP1

14 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Tcp1

14 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Tcp1

14 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\tcp1

7 of 13

Yes

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (10)

Drer\tcp1

13 of 14

Yes

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\cct6a

1 of 14

Drer\cct8

1 of 14

Drer\hspd1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\cct-1

14 of 14

Yes

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP004238

12 of 12

Yes

4 of 12

4 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\TCP-1

13 of 13

Yes

4 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\TCP1

13 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\cct1

12 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\groL

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CCT1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

CCT1^c03987

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TCP1; t-complex 1

14 of 14

186980

INTELLECTUAL DEVELOPMENTAL DISORDER WITH POLYMICROGYRIA AND SEIZURES; IDDPMGS

CCT2; chaperonin containing TCP1 subunit 2

4 of 14

605139

CCT7; chaperonin containing TCP1 subunit 7

4 of 14

605140

CCT4; chaperonin containing TCP1 subunit 4

3 of 14

CHAPERONIN CONTAINING T-COMPLEX POLYPEPTIDE 1, SUBUNIT 4; CCT4

CCT5; chaperonin containing TCP1 subunit 5

3 of 14

610150

NEUROPATHY, HEREDITARY SENSORY, WITH SPASTIC PARAPLEGIA, AUTOSOMAL RECESSIVE; HSNSP

autosomal recessive sensory neuropathy with spastic paraplegia

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CCT1 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - CCT2

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT4

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT5

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT7

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - Ost1

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - Pten

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - Pyk

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

CCT1 - RagA-B

anti tag coimmunoprecipitation, anti tag western blot

(Zhou et al., 2025)

CCT1 - Rheb

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Kim and Choi, 2019, Vinayagam et al., 2016)

CCT1 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

CCT1 - Vha36-1

anti tag coimmunoprecipitation, anti tag western blot

(Zhou et al., 2025)

CCT1 - csw

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

CCT1 - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - melt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - mst

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry, anti bait coimmunoprecipitation

(Palumbo et al., 2015)

CCT1 - pnut

anti bait coimmunoprecipitation, western blot

(Monzo et al., 2010)

CCT1 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT1 - wal

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

(UniProt, P12613 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67604

DroID - A comprehensive database of gene and protein interactions.

FBgn0003676

MIST (protein-protein) - An integrated Molecular Interaction Database

42649

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-75

Cytogenetic map

94B1-94B1

Sequence location

3R:22,489,105..22,491,439 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B1-94B1

Limits computationally determined from genome sequence between P{PZ}l(3)rQ178^rQ178 and P{lacW}Dph5^L4910

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

94B1-94B2

(determined by in situ hybridisation)

(Ursic and Ganetzky, 1988)

Experimentally Determined Recombination Data

Location

3-75

(FlyBase, 2016)

3-77.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

11325

w¹¹¹⁸; PBac{PB}CCT1^c03987/TM6B, Tb¹

62675

w¹¹¹⁸; PBac{IT.GAL4}CCT1^0157-G4/TM6B, Tb¹

32854

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00639}attP2

93972

w^*; TI{CRIMIC.TG4.1}CCT1^{CR60256-TG4.1}/TM3, Sb¹ Ser¹

Kyoto

115097

w¹¹¹⁸; PBac{602.P.SVS-1}CCT1^CPTI000037

NIG-Fly

5374R-2

P{NIG.5374R}2

HMS00639

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00639}attP2

VDRC

v34070

w¹¹¹⁸; P{GD10468}v34070/TM3

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (143)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE70560

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003676

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003676

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

Area matching Drosophila Tcp-1 gene, Acc. No. M21159.

(Davies, 2001.3.15)

Identified with: GH05123 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

(Zou et al., 2000)

EST GH05123 is chimeric; the 3' portion (BG632209) corresponds to part of T-cp1 on 3R, the 5' portion (AI107438) maps to 3L.

(FlyBase, 1996-)

Codes for a cDNA from D.melanogaster that shares 66% sequence identity with the mouse Tcp-1 gene encoding TCP1 (t-complex polypeptide 1).

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: CCT1 T-cp1

(Monzo et al., 2010)

Nomenclature comments

FlyBase curator comment: Renamed from 'T-cp1' to 'CCT1' to unify the nomenclature used for the CCT/TRiC subunits in FlyBase, following the nomenclature used in this paper (FBrf0210519) and other species.

(Monzo et al., 2010)

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

BEST:GH05123

CCT1

(Tsakiri et al., 2025, Lottes et al., 2024, Heigwer et al., 2023, Eickelberg et al., 2022, Perlegos et al., 2022, Evans et al., 2021, Pang et al., 2021, Hung et al., 2020, Toprak et al., 2020, Kim and Choi, 2019, Gene Disruption Project members, 2018-, Monzo et al., 2010)

CG5374

(Kolkhof et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Moutinho-Pereira et al., 2013, Papatsenko et al., 2011, Monzo et al., 2010, Ni et al., 2010.12.1, Hughes et al., 2008, Goshima et al., 2007)

Cct1

(Kong et al., 2019, Swevers et al., 2018, Kühnlein, 2010)

T-CP1

(Shirasaki et al., 2012)

T-cp1

(Titlow et al., 2023, Clandinin and Owens, 2016-, Ogneva et al., 2016, Rathore et al., 2016, Dent et al., 2015, Jia et al., 2015, Zang et al., 2015, Belozerov et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Kallappagoudar et al., 2010, Kong et al., 2010, Zhang et al., 2010, Geng and MacDonald, 2007, Stuart et al., 2007, Stroschein-Stevenson et al., 2006, Lindsley and Zimm, 1992)

T-cpl

(Kupriyanova et al., 2016, Begun, 2001, Begun and Whitley, 2000)

TCP-1α

(Monzo et al., 2010)

TCP1-alpha

(Palumbo et al., 2015)

TCP1α

(Min et al., 2017, Valakh et al., 2013)

TCPA_DROME

(Storm and Sonnhammer, 2003)

Tcp-1

Tcp1

(Ji et al., 2017, Völker-Albert et al., 2016, Goshima et al., 2007)

Tcp1-alpha

(Fisher et al., 2008)

Tcp1-like

(Moutinho-Pereira et al., 2013, McGraw et al., 2008)

cct1

(Ohhara et al., 2019, Seyres et al., 2016)

gh05123

(Zou et al., 2000)

tcp1

(Gill et al., 2015)

Name Synonyms

Chaperonin containing TCP1 subunit 1

T-complex 1

(Lindsley and Zimm, 1992)

Tcp1-like

(Monzo et al., 2010)

Secondary FlyBase IDs

FBgn0043836

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42649

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P12613

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P12613

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A4V391

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P12613

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003676

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42649/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003676

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003676

FlyMine - An integrated database for Drosophila genomics

FBgn0003676

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5374

MARRVEL_MODEL - MARRVEL (model organism gene)

42649

Linkouts

BioGRID - A database of protein and genetic interactions.

67604

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003676

DroID - A comprehensive database of gene and protein interactions.

FBgn0003676

DRSC - Results frm RNAi screens

FBgn0003676

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003676

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003676

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5374

Flygut - An atlas of the Drosophila adult midgut

FBgn0003676

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003676

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000969

MIST (protein-protein) - An integrated Molecular Interaction Database

42649

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

0.37

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