FlyBase Gene Report: Dmel\cyst

General Information

Symbol

Dmel\cyst

Species

D. melanogaster

Name

cysts

Annotation Symbol

CG10188

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032796

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

RhoGEF that maintains tissue polarity and integrity - together with RhoGEF4 plays roles to couple cytokinesis with de novo junction formation - acts via Rho1, participating in the neighbor mechanosensing response, promoting daughter-daughter cell membrane juxtaposition in preparation to de novo junction formation - Crumbs and its complex partner Cysts support myosin and apical constriction to ensure robust ingression dynamics - recruited to the apico-lateral membrane through interactions with the Crumbs complex and Bazooka/Par3 - required for cell intercalation in the extending ectoderm, where it activates Rho1 specifically at junctions - localization is restricted to adherens junctions and is under Gbeta13F/ Ggamma1 control - Myosin VI promotes recruitment of the heterotrimeric Galpha12 protein to E-cadherin, where it signals for p114 RhoGEF to activate RhoA (Interactive Fly, cyst )

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Dp114RhoGEF

Key Links

Genomic Location

Cytogenetic map

37E4-37E5

Sequence location

2L:19,502,335..19,508,764 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:19502335..19508764

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from direct assay

(di Pietro et al., 2023)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from sequence or structural similarity with FLYBASE:RhoGEF2; FB:FBgn0023172

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002677784

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR000219

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in mitotic cytokinesis, division site positioning

inferred from expression pattern

(di Pietro et al., 2023)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of Rho protein signal transduction

inferred from sequence or structural similarity with FLYBASE:RhoGEF2; FB:FBgn0023172

(Gene Ontology Curators, 2002-)

involved_in regulation of Rho protein signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN002677784

(GO Reference Genome Project, 2011-)

involved_in small GTPase-mediated signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN002677784

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

is_active_in cleavage furrow rim

inferred from direct assay

(di Pietro et al., 2023)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -: RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\cyst for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PBE1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.44

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

cyst-RA

FBtr0081283

NM_165305

5302

1309

cyst-RB

FBtr0081282

NM_136133

5216

1309

cyst-RC

FBtr0329963

NM_001273659

5242

1319

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

cyst-PA

FBpp0080821

145.0

1309

7.73

FBpp0080820

145.0

1309

7.73

FBpp0302998

146.1

1319

7.63

M9PBE1

NP_001260588

AGB93123

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1309 aa isoforms: cyst-PA, cyst-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cyst using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10188_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.05

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P3

leg | ubiquitous

(Greenberg and Hatini, 2011)

Additional Descriptive Data

Expression in stage P3 pupal leg discs is ubiquitous.

(Greenberg and Hatini, 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in cleavage furrow rim

inferred from direct assay

(di Pietro et al., 2023)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\cyst in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\cyst

View exonic expression by tissue for Dmel\cyst

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35237/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032796

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032796

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032796

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10188

Flygut - An atlas of the Drosophila adult midgut

FBgn0032796

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 27 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of cyst

Transgenic constructs containing regulatory region of cyst

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)Exel7076

(Ryder, 2004.4.26)

Df(2L)Exel8041

(Ryder, 2004.4.26)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

cyst¹

lethal - all die during embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^GL00651

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

cyst^GD6891

viable, with Scer\GAL4^Mef2.PR

cyst^GD6891

viable, with Scer\GAL4^pnr-MD237

cyst^GD6891

viable, with Scer\GAL4^tin.CΔ4

cyst^GD6891

Other Phenotypes

Allele

abnormal cell polarity | embryonic stage | maternal effect, with Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

some die during embryonic stage | maternal effect

cyst¹

Phenotype manifest in

Allele

adherens junction | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

ectoderm | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^GL00651

embryo, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^GL00651

embryonic/larval cuticle | embryonic stage | maternal effect

cyst¹

embryonic/larval cuticle | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

embryonic epidermis | maternal effect, with Df(2L)BSC301, Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

embryonic epidermis | maternal effect | progressive, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

embryonic head | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^UASp.R

ventral ectoderm | embryonic stage | maternal effect | progressive, with Scer\GAL4^{VP16.mat.αTub67C}

cyst^HMS01750

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (52)

Hsap\ARHGEF2

10 of 14

Yes

Hsap\ARHGEF18

10 of 14

Yes

Hsap\ARHGEF28

9 of 14

Yes

Hsap\AKAP13

6 of 14

Yes

Hsap\ARHGEF3

3 of 14

Yes

Hsap\ARHGEF11

3 of 14

Hsap\ARHGEF12

3 of 14

Hsap\NET1

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Arhgef2

10 of 14

Yes

Rnor\Arhgef18

9 of 14

Yes

Rnor\Arhgef28

9 of 14

Yes

Rnor\Akap13

6 of 14

Yes

Rnor\Arhgef3

3 of 14

Yes

Rnor\Arhgef11

3 of 14

Rnor\Arhgef12

3 of 14

Rnor\Net1

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (33)

Mmus\Arhgef2

10 of 14

Yes

Mmus\Arhgef18

10 of 14

Yes

Mmus\Arhgef28

9 of 14

Yes

Mmus\Akap13

5 of 14

Yes

Mmus\Arhgef1

3 of 14

Mmus\Arhgef3

3 of 14

Yes

Mmus\Arhgef11

3 of 14

Mmus\Arhgef12

3 of 14

Mmus\Net1

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (32)

Xtro\arhgef18

8 of 13

Yes

Xtro\arhgef2

5 of 13

Yes

Xtro\arhgef28

5 of 13

Yes

Xtro\akap13

2 of 13

Yes

Xtro\asb13

2 of 13

Yes

Xtro\arhgef3.1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\c16orf87

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\mcf2l

1 of 13

Xtro\mcts1

1 of 13

Xtro\net1

1 of 13

Yes

Xtro\ngef

1 of 13

Xtro\nrip1

1 of 13

Yes

Xtro\plekhg2

1 of 13

Xtro\plekhg5

1 of 13

Xtro\plekhg7

1 of 13

Yes

Xtro\prex1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (44)

Drer\arhgef2

10 of 14

Yes

Drer\arhgef18a

10 of 14

Yes

Drer\arhgef1a

9 of 14

Yes

Drer\arhgef18b

9 of 14

Yes

Drer\arhgef28a

9 of 14

Yes

Drer\akap13

5 of 14

Yes

Drer\si:dkey-172h23.2

4 of 14

Yes

Drer\arhgef1b

3 of 14

Drer\arhgef12a

3 of 14

Drer\net1

3 of 14

Yes

Drer\akap13

2 of 14

Yes

Drer\arhgef3

2 of 14

Yes

Drer\arhgef3l

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC110438753

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\Y105E8A.25

4 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (13)

Agam\AgaP_AGAP007712

12 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\ROM1

3 of 13

Yes

Scer\ROM2

3 of 13

Yes

Scer\TUS1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\rgf1

3 of 12

Yes

Spom\rgf3

3 of 12

Yes

Spom\rgf2

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:cyst. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

RhoGEF2

4 of 13

RhoGEF4

3 of 13

CG42674

2 of 13

Exn

2 of 13

RhoGEF64C

2 of 13

CG43102

1 of 13

GEFmeso

1 of 13

pbl

1 of 13

RhoGEF3

1 of 13

sif

1 of 13

Vav

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

ARHGEF18; Rho/Rac guanine nucleotide exchange factor 18

model of retinitis pigmentosa 78

IEA

(FlyBase, 2019-)

ARHGEF2; Rho/Rac guanine nucleotide exchange factor 2

model of neurodevelopmental disorder with midbrain and hindbrain malformations

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

cyst^HMS01750

exacerbates amyotrophic lateral sclerosis type 21

modeled by Hsap\MATR3^{S85C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{F115C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

cyst^GLC01356

exacerbates amyotrophic lateral sclerosis type 21

modeled by Hsap\MATR3^{S85C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{F115C.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

modeled by Hsap\MATR3^{T622A.UAS.cZa.Tag:FLAG}

(Zhao et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARHGEF2; Rho/Rac guanine nucleotide exchange factor 2

10 of 14

607560

NEURODEVELOPMENTAL DISORDER WITH MIDBRAIN AND HINDBRAIN MALFORMATIONS; NEDMHM

neurodevelopmental disorder with midbrain and hindbrain malformations

ARHGEF18; Rho/Rac guanine nucleotide exchange factor 18

10 of 14

616432

RETINITIS PIGMENTOSA 78; RP78

retinitis pigmentosa 78

ARHGEF28; Rho guanine nucleotide exchange factor 28

9 of 14

612790

AKAP13; A-kinase anchoring protein 13

6 of 14

604686

ARHGEF3; Rho guanine nucleotide exchange factor 3

3 of 14

612115

ARHGEF11; Rho guanine nucleotide exchange factor 11

3 of 14

605708

ARHGEF12; Rho guanine nucleotide exchange factor 12

3 of 14

604763

NET1; neuroepithelial cell transforming 1

3 of 14

606450

PLEKHG6; pleckstrin homology and RhoGEF domain containing G6

3 of 14

611743

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cyst

anti tag coimmunoprecipitation, anti tag western blot

(Silver et al., 2019)

cyst - Patj

anti tag coimmunoprecipitation, anti tag western blot

(Silver et al., 2019)

cyst - baz

anti tag coimmunoprecipitation, anti tag western blot

(Silver et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cyst

enhanceable

aPKC

(Silver et al., 2019)

cyst

suppressible

l(2)gl

(Silver et al., 2019)

cyst

suppressible

scrib

(Silver et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brm

suppressible

cyst

(Tegeder et al., 2019)

Rho1

suppressible

cyst

(Silver et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61214

DroID - A comprehensive database of gene and protein interactions.

FBgn0032796

MIST (protein-protein) - An integrated Molecular Interaction Database

35237

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

37E4-37E5

Sequence location

2L:19,502,335..19,508,764 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37E4-37E5

Limits computationally determined from genome sequence between P{EP}dnt^EP2158 and P{EP}EP623&P{PZ}spi⁰¹⁰⁶⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-53.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33047

y¹ v¹; P{TRiP.HMS00713}attP2

79309

y¹ w^*; TI{CRIMIC.TG4.1}cyst^{CR00834-TG4.1}/SM6a

86414

y¹ w^*; TI{CRIMIC.TG4.1}cyst^{CR01482-TG4.1}/SM6a

41578

y¹ sc^* v¹ sev²¹; P{TRiP.GL00650}attP2

41579

y¹ sc^* v¹ sev²¹; P{TRiP.GL00651}attP40

38292

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01750}attP40

38942

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01856}attP40

NIG-Fly

HMS00713

y¹ v¹; P{TRiP.HMS00713}attP2

M2L-1013

y¹ w¹¹¹⁸; cyst^SK3 P{neoFRT}40A/CyO-Tb

M2L-1014

y¹ w¹¹¹⁸; cyst^SK5 P{neoFRT}40A/CyO-Tb

VDRC

v330275

P{VSH330275}attP40

v103391

P{KK100404}VIE-260B

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (128)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH26723

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032796

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032796

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: cyst CG10188

(Silver et al., 2019)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Name Synonyms

cysts

(Silver et al., 2019)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35237

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PBE1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PBE1

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35237/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032796

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032796

FlyMine - An integrated database for Drosophila genomics

FBgn0032796

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10188

MARRVEL_MODEL - MARRVEL (model organism gene)

35237

Linkouts

BioGRID - A database of protein and genetic interactions.

61214

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032796

DroID - A comprehensive database of gene and protein interactions.

FBgn0032796

DRSC - Results frm RNAi screens

FBgn0032796

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10188_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032796

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032796

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10188

Flygut - An atlas of the Drosophila adult midgut

FBgn0032796

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032796

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009420

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

35237

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (53)

The following external sites may use different assemblies or annotations than FlyBase.

-1.05

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