FlyBase Gene Report: Dmel\Pgk

General Information

Symbol

Dmel\Pgk

Species

D. melanogaster

Name

Phosphoglycerate kinase

Annotation Symbol

CG3127

Feature Type

protein_coding_gene

FlyBase ID

FBgn0250906

Gene Model Status

Current

Stock Availability

26 publicly available

Enzyme Name (EC)

phosphoglycerate kinase (2.7.2.3)

Gene Summary

Phosphoglycerate kinase (Pgk) encodes an enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate producing ATP. It is involved in glycolysis and while the reverse reaction is involved in gluconeogenesis. [Date last reviewed: 2024-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

l(2)23Ac

Key Links

Genomic Location

Cytogenetic map

23A3-23A3

Sequence location

2L:2,746,880..2,753,068 [-]

Recombination map

2-7

RefSeq locus

NT_033779 REGION:2746880..2753068

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ADP binding

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P00559

(Gene Ontology Curators, 2002-)

enables phosphoglycerate kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001576, InterPro:IPR015824, InterPro:IPR015911, InterPro:IPR036043

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P00560

(Gene Ontology Curators, 2002-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in canonical glycolysis

inferred from mutant phenotype

(Zappia et al., 2023)

involved_in chemical synaptic transmission

inferred from direct assay

(Wang et al., 2004)

involved_in muscle cell cellular homeostasis

inferred from genetic interaction with UniProtKB:A0A0C4DHF6

(Kucherenko et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in gluconeogenesis

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P16617

(Gene Ontology Curators, 2002-)

involved_in glycolytic process

inferred from sequence or structural similarity with UniProtKB:P00560

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001576, InterPro:IPR015824, InterPro:IPR015911, InterPro:IPR036043

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in M band

inferred from direct assay

(Sullivan et al., 2003)

located_in Z disc

inferred from direct assay

(Sullivan et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000150497

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P09411

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

UNCLASSIFIED KINASES

Pathway (FlyBase)

GLYCOLYSIS

Protein Family (UniProt)

Belongs to the phosphoglycerate kinase family. (Q01604)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

phosphoglycerate kinase activity

(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP (2.7.2.3)

RHEA 14801:

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED KINASES -: This group comprises kinases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

GLYCOLYSIS -: Glycolysis converts glucose into ATP and pyruvate in the cytosol. The overall reaction is glucose + 2 ADP + 2Pi + 2 NAD+ -> 2 pyruvate + 2 ATP + 2 NADH 2H+ + 2 H2O and involves 10 enzymatic steps. In the presence of oxygen, pyruvate is transported into the mitochondrion where, after oxidation into acetyl-CoA, it enters the tricarboxylic acid cycle (TCA cycle). In anaerobic conditions, pyruvate is reduced to lactate in the cytosol. Although glucose, trehalose and glycogen are the main entry points for glycolysis in D. melanogaster, fructose, galactose, mannose and glycerol can also enter the glycolytic pathway to be converted into pyruvate. (Adapted from PMID:33941515 and FBrf0144936.)

Protein Function (UniProtKB)

Enzyme of the glycolytic pathway (Probable). Glycolysis is essential in glial cells but not in neurons; neurons rely on the citric acid cycle for their energy needs, and on lactate and alanine secreted into the hemolymph by glial cells to fuel it (PubMed:26235423).

(UniProt, Q01604)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Pgk: Phosphoglycerate kinase

Structural gene for 3-phosphoglycerate kinase [3-PGK (E.C. 2.7.2.3)]. Three electrophoretic variants (fast, intermediate, slow) isolated by Chew and Cooper (1973), the heterozygotes showing no hybrid band; two electrophoretic variants isolated by Volker et al. (1979).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pgk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q01604)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pgk-RA

FBtr0077739

NM_057328

1486

415

Pgk-RB

FBtr0330716

NM_001273027

1860

415

Pgk-RC

FBtr0330717

NM_001273028

1867

415

Pgk-RD

FBtr0330718

NM_001273029

1865

415

Pgk-RE

FBtr0334162

NM_001273030

1893

415

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Roselli-Rehfuss et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pgk-PA

FBpp0077419

43.9

415

7.50

FBpp0303560

43.9

415

7.50

FBpp0303561

43.9

415

7.50

FBpp0303562

43.9

415

7.50

FBpp0306279

43.9

415

7.50

Q01604

NP_001259959

AGB92496

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

415 aa isoforms: Pgk-PA, Pgk-PB, Pgk-PC, Pgk-PD, Pgk-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

415 (aa); 44 (kD)

(Roselli-Rehfuss et al., 1992)

Comments

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, Q01604)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pgk using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.92

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

embryonic stage 15

somatic muscle cell

(Tixier et al., 2013)

Additional Descriptive Data

Maternally derived Pgk transcripts are found at substantial levels in early embryos. Transcript levels decline until mid-embryogenesis and then rise. Transcript levels remain high during larval stages, decline at pupariation, rise again at emergence, and remain high in the adult.

(Roselli-Rehfuss et al., 1992)

Transcript levels are unaltered by dietary variations in the level of glucose, fructose, starch, or ethanol. A small increase is seen on 5% sucrose vs. 0.3% sucrose.

(Lissemore et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

muscle cell

(Tixier et al., 2013)

adult stage

flight muscle cell

• myofibril | restricted

(Sullivan et al., 2003)

flight muscle cell

• sarcomere | restricted

(Sullivan et al., 2003)

flight muscle cell

• M band

(Sullivan et al., 2003)

flight muscle cell

• Z disc

(Sullivan et al., 2003)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Glycolytic enzymes, including Pgk protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands.

(Sullivan et al., 2003)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in M band

inferred from direct assay

(Sullivan et al., 2003)

located_in Z disc

inferred from direct assay

(Sullivan et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pgk in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pgk

View exonic expression by tissue for Dmel\Pgk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250906

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33461/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250906

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250906

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3127

Flygut - An atlas of the Drosophila adult midgut

FBgn0250906

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Pgk

Stages(s) 7-8
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pgk

Transgenic constructs containing regulatory region of Pgk

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2L)C144

(Wang et al., 2004, DiAntonio et al., 1993)

Df(2L)DTD2

(DiAntonio et al., 1993)

Df(2L)Exel6008

(Ryder, 2004.4.26)

Df(2L)Exel6277

(Ryder, 2004.4.26)

Df(2L)N19

(Littleton and Bellen, 1994)

Gene not disrupted in

Df(2L)dpp-H34

(Littleton and Bellen, 1994, DiAntonio et al., 1993)

Df(2L)N6

(Littleton and Bellen, 1994)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^da.PU

Pgk^KK102941

lethal - all die before end of pupal stage | recessive

Pgk^T63

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Pgk^GD10211

partially lethal, with Scer\GAL4^tin.CΔ4

Pgk^GD10211

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pgk^GD10211

viable, with Scer\GAL4^nSyb.PS

Pgk^KK102941

viable, with Scer\GAL4^pnr-MD237

Pgk^GD10211

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^apolpp.PU

Pgk^HMS00030

abnormal locomotor behavior

Pgk^nubian

abnormal locomotor behavior, with Scer\GAL4^repo.PU

Pgk^KK102941

abnormal locomotor behavior | adult stage, with Scer\GAL4^ple.PF

Pgk^GL00101

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^ple.PF

Pgk^KK102941

abnormal locomotor behavior | larval stage, with Scer\GAL4^ple.PF

abnormal neurophysiology | heat sensitive

Pgk^nubian

decreased cell number | adult stage, with Scer\GAL4^ple.PF

Pgk^KK102941

decreased cell number | adult stage | progressive, with Scer\GAL4^ple.PF

Pgk^GL00101

decreased cell size | larval stage, with Scer\GAL4^apolpp.PU

Pgk^HMS00030

decreased size | adult stage, with Scer\GAL4^C-765

decreased sleep, with Scer\GAL4^ple.PF

Pgk^GL00101

decreased sleep | progressive, with Scer\GAL4^ple.PF

Pgk^KK102941

electrophoretic variant

Pgk⁴

electrophoretic variant | fast

Pgk³

electrophoretic variant | intermediate

Pgk²

electrophoretic variant | slow

Pgk¹

flightless, with Scer\GAL4^repo.PU

Pgk^KK102941

paralytic

Pgk^nubian

short lived

Pgk^nubian

some die during pupal stage | recessive

Pgk^T63

Phenotype manifest in

Allele

dopaminergic PPL1 neuron | adult stage | decreased number, with Scer\GAL4^ple.PF

Pgk^KK102941

dopaminergic PPM1 neuron | adult stage | decreased number, with Scer\GAL4^ple.PF

dopaminergic PPM2 neuron | adult stage | decreased number, with Scer\GAL4^ple.PF

embryonic/larval fat body | larval stage, with Scer\GAL4^apolpp.PU

Pgk^HMS00030

embryonic myoblast, with Scer\GAL4^kirre.PU

Pgk^KK102941

larval somatic muscle cell, with Scer\GAL4^kirre.PU

Pgk^KK102941

mitochondrion, with Scer\GAL4^en-e16E

Pgk^NIG.3127R

rhabdomere | adult stage, with Scer\GAL4^GMR.PU

Pgk^RNAi.UAS.cUa

synapse

Pgk^nubian

type I bouton

Pgk^nubian

visceral muscle fiber

Pgk^nubian

wing, with Scer\GAL4^C-765

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PGK1

14 of 14

Yes

Hsap\PGK2

13 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Pgk1

13 of 14

Yes

Rnor\Pgk2

13 of 14

Yes

Rnor\RGD1560402

10 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Pgk1

14 of 14

Yes

Mmus\Pgk2

12 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\pgk1

8 of 13

Yes

Danio rerio (Zebrafish) (1)

Drer\pgk1

14 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\pgk-1

14 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP008802

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (3)

Atha\AT1G56190

11 of 13

Yes

Atha\PGK1

11 of 13

Yes

Atha\PGK

10 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\PGK1

13 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pgk1

12 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\pgk

10 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pgk. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Pgk2

12 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Parkinson-like model, PGK-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Pgk^GL00101

model of Parkinson's disease

CEA

(Shimizu et al., 2020)

Pgk^KK102941

model of Parkinson's disease

CEA

(Shimizu et al., 2020)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PGK1; phosphoglycerate kinase 1

model of phosphoglycerate kinase 1 deficiency

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Pgk^KG07478

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011)

Pgk^KK102941

model of Parkinson's disease

is ameliorated by Sod1^UAS.cWa

(Shimizu et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PGK1; phosphoglycerate kinase 1

14 of 14

PHOSPHOGLYCERATE KINASE 1; PGK1

PHOSPHOGLYCERATE KINASE 1 DEFICIENCY

phosphoglycerate kinase 1 deficiency

PGK2; phosphoglycerate kinase 2

13 of 14

172270

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pgk - Adh

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Aldo

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - AnxB10

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Ar2

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Argk1

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG14715

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG16826

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG31343

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG32473

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG4598

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG5500

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - CG5506

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Dld

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Eno

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Fkbp14

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Gapdh2

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Glyp

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Gpdh1

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Gtpx

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Lsp2

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Mdh2

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Moe

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Pgk - Pgam1

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Prx6c

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Scp1

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Sh3β

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Stip1

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - TER94

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Uch

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - Yp2

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - aub

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

Pgk - awd

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - cactin

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Chen et al., 2024)

Pgk - chic

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - ftz-f1

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Pgk - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

Pgk - scu

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pgk

suppressible

Sod1

(Shimizu et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Pgk

(Kucherenko et al., 2008)

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, Q01604 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59686

DroID - A comprehensive database of gene and protein interactions.

FBgn0250906

MIST (protein-protein) - An integrated Molecular Interaction Database

33461

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

GLYCOLYSIS

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-7

Cytogenetic map

23A3-23A3

Sequence location

2L:2,746,880..2,753,068 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23A3-23A3

Limits computationally determined from genome sequence between P{PZ}dpp¹⁰⁶³⁸ and P{EP}EP832

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

23A-23B

(determined by in situ hybridisation)

(Louis et al., 1997)

23A-23B

(EDGP Project Members, 1994-)

23A1-23A2

(determined by in situ hybridisation)

(Roselli-Rehfuss et al., 1992)

Experimentally Determined Recombination Data

Location

2-7

(FlyBase, 2016)

2-6.2

(Comeron et al., 2012)

(Choudhary et al., 1992)

2-5.9

(Voelker et al., 1979)

Left of (cM)

(Voelker et al., 1979)

Right of (cM)

(Voelker et al., 1979)

Notes

Stocks and Reagents

Stocks (26)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33632

y¹ v¹; P{TRiP.HMS00030}attP2

65476

w¹¹¹⁸; PBac{IT.GAL4}Pgk^1147-G4/CyO

14487

y¹; P{SUPor-P}Pgk^KG06443/CyO; ry⁵⁰⁶

15113

y¹; P{SUPor-P}Pgk^KG07478/CyO; ry⁵⁰⁶

10887

11505

3127R-3

3127R-4

HMS00030

y¹ v¹; P{TRiP.HMS00030}attP2

HMS00031

y¹ v¹; P{TRiP.HMS00031}attP2

VDRC

v110081

P{KK102941}VIE-260B

v33797

w¹¹¹⁸; P{GD10211}v33797

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (54)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH07208

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0250906

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0250906

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Sullivan et al., 2003)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Pgk mutants display reduced lifespan, abnormal motor behavior, altered synaptic structure, and defective neurotransmitter release.

(Wang et al., 2004)

Comparative analysis of Pgk sequences from other species.

(Fitzgerald et al., 1996)

Identification: Screen for lethals heterozygous with Df(2L)C144.

(DiAntonio et al., 1993)

Pgk gene cloned by homology to human Pgk. DNA sequence and temporal pattern of expression determined.

(Roselli-Rehfuss et al., 1992)

Modulation of Gpdh, Ald, Pgk and RpL32 proteins and their transcript levels was investigated in larvae. No increase in transcript level or PGK activity was seen with an increase in sucrose, glucose, fructose, starch or dietary ethanol levels.

(Lissemore et al., 1990)

No null alleles reported out of a total of 702 alleles collected in North Carolina (Langley et al., 1981).

Structural gene for 3-phosphoglycerate kinase (3-PGK). Three electrophoretic variants (fast, intermediate, slow) isolated by Chew and Cooper (1973), the heterozygotes showing no hybrid band; two electrophoretic variants isolated by Voelker et al. (1979).

Relationship to Other Genes

Source for database merge of

Source for merge of: Pgk l(2)23AB3

(Wang et al., 2004)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

169C9S

(Louis et al., 1997)

CG3127

(Choo et al., 2015, Cammarato et al., 2011, Ni et al., 2011.7.19, O'Keefe et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009)

ESTS:169C9S

PGK

(Serebrovska et al., 2025, Beghelli et al., 2024, Böttcher et al., 2014, Saj et al., 2010, Sullivan et al., 2003, Choudhary et al., 1992)

Pgk

(Zaru and Marygold, 2025, Aziz et al., 2024, Cheng et al., 2024, De Groef et al., 2024, Li et al., 2024, Rodríguez-Vázquez et al., 2024, Rojas-Ríos et al., 2024, Vesala et al., 2024, Pang et al., 2023, Zappia et al., 2023, Hunt and Demontis, 2022, Zhu et al., 2022, Lebo et al., 2021, Hope et al., 2020, Li et al., 2020, Shimizu et al., 2020, Cai et al., 2019, Hudry et al., 2019, Luhur et al., 2019, van den Ameele and Brand, 2019, Wong et al., 2019, Ding et al., 2018, Park et al., 2018, Cogni et al., 2017, Transgenic RNAi Project members, 2017-, Yoon et al., 2017, Clandinin and Owens, 2016-, Elgart et al., 2016, Kockel et al., 2016, Choo et al., 2015, Chen et al., 2014, Lavington et al., 2014, Luck et al., 2014, Mukherjee et al., 2014, Yasunaga et al., 2014, Beckett et al., 2013, Kwon et al., 2013, Nirala et al., 2013, Tixier et al., 2013, Tixier et al., 2013, Eanes, 2011, Kucherenko et al., 2011, Marrone et al., 2011, O'Keefe et al., 2011, Kallappagoudar et al., 2010, Meadows et al., 2010, Dworkin et al., 2009, Kucherenko et al., 2008, Flowers et al., 2007, Zhang et al., 2007, Rehwinkel et al., 2006, Sandmann et al., 2006)

anon-sts18

l(2)23AB3

l(2)23Ac

(Wang et al., 2004, Littleton and Bellen, 1994)

pgk

(Sharma et al., 2017, Kanda et al., 2011, Wang et al., 2004, Juge et al., 2002, Wilhelm et al., 2000)

pgk1

(Martínez-Morentin et al., 2015)

Name Synonyms

Phosphoglycerate kinase

(Rice et al., 2020, Reid and O'Brochta, 2016, Zheng et al., 2016, Feng et al., 2015, Volkenhoff et al., 2015, Cammarato et al., 2011, Kucherenko et al., 2011, Varma and Mishra, 2011, Sambandan et al., 2006)

lethal (2) 23AB3

phosphoglycerate kinase

(Kanda et al., 2011, O'Keefe et al., 2011, Wang et al., 2004, Sullivan et al., 2003)

phosphoglycerokinase

(Pierce et al., 2001)

Secondary FlyBase IDs

FBgn0003075
FBgn0014416
FBgn0013525

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 75 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33461

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q01604

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q01604

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PCE0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q01604

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250906

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33461/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250906

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250906

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3127

MARRVEL_MODEL - MARRVEL (model organism gene)

33461

Linkouts

BioGRID - A database of protein and genetic interactions.

59686

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0250906

DroID - A comprehensive database of gene and protein interactions.

FBgn0250906

DRSC - Results frm RNAi screens

FBgn0250906

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0250906

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3127

Flygut - An atlas of the Drosophila adult midgut

FBgn0250906

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0250906

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015540

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

33461

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (151)

The following external sites may use different assemblies or annotations than FlyBase.

-0.92

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