FB2020_06, released Dec 22, 2020

Gene: Dmel\Ca-Ma2d

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General Information
Symbol
Dmel\Ca-Ma2d
Species
D. melanogaster
Name
Ca2+ channel Muscle-specific alpha2/delta subunit
Annotation Symbol
CG42817
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261999
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

BG:DS07473.1

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:16,231,475..16,241,527 [+]
Recombination map

2-52

RefSeq locus
NT_033779 REGION:16231475..16241527
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables voltage-gated calcium channel activity
inferred from biological aspect of ancestor with PANTHER:PTN000018330
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in sarcoplasmic reticulum
inferred from direct assay
(Reuveny et al., 2018)
located_in T-tubule
inferred from direct assay
(Reuveny et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of voltage-gated calcium channel complex
inferred from biological aspect of ancestor with PANTHER:PTN000018330
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
VOLTAGE-GATED CALCIUM CHANNEL SUBUNITS -
Voltage-gated Ca2+ channels (CACN) allow the influx of extracellular Ca2+ in response to plasma membrane depolarization. CACNs are hetero-oligomeric assemblies of a pore-forming α1 subunit plus other structural and accessory subunits. (Adapted from FBrf0218524 and PMID:21746798).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Ca-Ma2d for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
(FlyBase Genome Annotators, 2010)
protein_coding_gene
(FlyBase Genome Annotators, 2010)
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ca-Ma2d-RA
FBtr0303797
NM_165149
4919
1215
Ca-Ma2d-RB
FBtr0332356
NM_001273557
5790
1215
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Ca-Ma2d-PA
FBpp0292804
140.6
1215
4.85
NP_723955
AAN10933
Ca-Ma2d-PB
FBpp0304633
140.6
1215
4.85
NP_001260486
AGB93021
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1215 aa isoforms: Ca-Ma2d-PA, Ca-Ma2d-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ca-Ma2d using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval muscle system
(Reuveny et al., 2018)
embryonic/larval muscle system
(Reuveny et al., 2018)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Ca-Ma2d protein localizes to the sarcoplasmic reticulum and T-tubules, and accumulates at the myonuclear membrane.

(Reuveny et al., 2018)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in sarcoplasmic reticulum
inferred from direct assay
(Reuveny et al., 2018)
located_in T-tubule
inferred from direct assay
(Reuveny et al., 2018)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ca-Ma2d in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ca-Ma2d
Transgenic constructs containing regulatory region of Ca-Ma2d
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\CACNA2D3
9 of 15
Yes
No
Hsap\CACNA2D4
9 of 15
Yes
No
Hsap\CACNA2D1
4 of 15
No
No
Hsap\CACNA2D2
4 of 15
No
Yes
Hsap\CACHD1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Cacna2d3
8 of 15
Yes
No
Mmus\Cacna2d4
8 of 15
Yes
No
Mmus\Cacna2d2
4 of 15
No
Yes
Mmus\Cacna2d1
3 of 15
No
No
Mmus\Cachd1
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
Rnor\Cacna2d3
9 of 13
Yes
No
Rnor\Cacna2d4
5 of 13
No
No
Rnor\Cacna2d2
4 of 13
No
Yes
Rnor\Cacna2d1
3 of 13
No
Yes
Rnor\Cachd1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
Xtro\cacna2d4
5 of 12
Yes
No
Xtro\cacna2d1
3 of 12
No
Yes
Xtro\cacna2d2
3 of 12
No
Yes
Xtro\cachd1
1 of 12
No
No
Xtro\cacna2d3
1 of 12
No
No
Danio rerio (Zebrafish) (9)
Drer\cacna2d3
8 of 15
Yes
No
Drer\cacna2d4b
8 of 15
Yes
No
Drer\cacna2d4a
5 of 15
No
No
Drer\cacna2d1a
4 of 15
No
Yes
Drer\cacna2d2b
4 of 15
No
Yes
Drer\cacna2d2a
3 of 15
No
Yes
Drer\cacna2d3a
3 of 15
No
No
Drer\cachd1
1 of 15
No
No
Drer\cacna2d1
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\unc-36
8 of 15
Yes
No
Cele\tag-180
2 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900WL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000957
Drosophila simulans
Dsim\GD24043
Drosophila sechellia
Dsec\GM18657
Drosophila erecta
Dere\GG25194
Drosophila yakuba
Dyak\GE21365
Drosophila ananassae
Dana\GF14941
Drosophila pseudoobscura pseudoobscura
Dpse\GA11645
Drosophila persimilis
Dper\GL21046
Drosophila willistoni
Dwil\GK18111
Drosophila virilis
Dvir\GJ18056
Drosophila mojavensis
Dmoj\GI17380
Drosophila grimshawi
Dgri\GH10400
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500GL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013047
Glossina morsitans
Tsetse fly
Gmor\GMOY001039
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02561
Mayetiola destructor
Hessian fly
Mdes\Mdes001071
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005392
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011040
Anopheles darlingi
American malaria mosquito
Adar\ADAC001980
Anopheles gambiae
Malaria mosquito
Agam\AGAP009579
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001793
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0107 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA003639
Bombyx mori
Silkmoth
Bmor\BGIBMGA003640
Danaus plexippus
Monarch butterfly
Dple\DPOGS215762-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL014118
Apis florea
Little honeybee
Aflo\3877647348
Apis florea
Little honeybee
Aflo\2704346340
Apis mellifera
Western honey bee
Amel\GB44648
Apis mellifera
Western honey bee
Amel\GB44647
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP19319
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP19318
Bombus terrestris
Buff-tailed bumblebee
Bter\15216667
Bombus terrestris
Buff-tailed bumblebee
Bter\15216662
Linepithema humile
Argentine ant
Lhum\LH20278
Linepithema humile
Argentine ant
Lhum\LH20279
Linepithema humile
Argentine ant
Lhum\LH20276
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002723
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006028
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006025
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10154
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06616
Tribolium castaneum
Red flour beetle
Tcas\TC034736
Tribolium castaneum
Red flour beetle
Tcas\TC004591
Pediculus humanus
Human body louse
Phum\PHUM308330
Pediculus humanus
Human body louse
Phum\PHUM308320
Rhodnius prolixus
Kissing bug
Rpro\RPRC013647
Rhodnius prolixus
Kissing bug
Rpro\RPRC015038
Cimex lectularius
Bed bug
Clec\CLEC013620
Cimex lectularius
Bed bug
Clec\CLEC008838
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005076
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002652
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10863
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10864
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00YH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR000568
Ixodes scapularis
Black-legged tick
Isca\ISCW019599
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63763.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63764.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63765.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63762.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65775.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM74283.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM74284.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur28g00940
Tetranychus urticae
Two-spotted spider mite
Turt\tetur23g01660
Tetranychus urticae
Two-spotted spider mite
Turt\tetur23g01920
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_317041
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
stj
4 of 10
stol
4 of 10
CG16868
2 of 10
CG42818
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CACNA2D3; calcium voltage-gated channel auxiliary subunit alpha2delta 3
    9 of 15
    606399
        CACNA2D4; calcium voltage-gated channel auxiliary subunit alpha2delta 4
        9 of 15
        608171
        CACNA2D1; calcium voltage-gated channel auxiliary subunit alpha2delta 1
        4 of 15
        114204
            CACNA2D2; calcium voltage-gated channel auxiliary subunit alpha2delta 2
            4 of 15
            607082
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Interactions Browser
              View in Interactions Browser
              Please see the Physical Interaction reports below for full details
              protein-protein
              Physical Interaction
              Assay
              References
              Ca-Ma2d - Amph
              anti tag coimmunoprecipitation, western blot
              (Reuveny et al., 2018)
              Summary of Genetic Interactions
              esyN Network Diagram
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              External Data
              Linkouts
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              Pathways
              Signaling Pathways (FlyBase)
              Metabolic Pathways
              External Data
              Linkouts
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              2L
              Recombination map

              2-52

              Cytogenetic map
              35F1-35F1
              Sequence location
              2L:16,231,475..16,241,527 [+]
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              35F1-35F1
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              Experimentally Determined Recombination Data
              Location

              2-52

              (FlyBase, 2016)

              2-49.3

              (Comeron et al., 2012)
              Left of (cM)
              Right of (cM)
              Notes

              Gene order: In direction of increasing cytology: CG31819+ CG12455+ PRL-1-

              (Ashburner et al., 1999)
              Stocks and Reagents
              Stocks (4)
              Genomic Clones (25)
              cDNA Clones (15)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequenced
              BDGP DGC clones
              Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                 

                monoclonal

                (Reuveny et al., 2018)

                polyclonal

                (Reuveny et al., 2018)
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of

                Source for identity of: Ca-Ma2d CG42817

                (Reuveny et al., 2018)
                Source for database merge of

                Source for merge of: BG:DS07473.1 CG12455

                (Bayraktaroglu, 2000.4.8)

                Source for merge of: CG12455 anon-WO0138359.9

                (FlyBase, 1996-)
                Additional comments

                Source for split of: CG12455

                (FlyBase Genome Annotators, 2010)

                Annotation CG12455 split into CG42817 and CG42818 in release 5.30 of the genome annotation.

                (FlyBase Genome Annotators, 2010)

                Source for merge of CG12455 anon-WO0138359.9 was sequence comparison ( date:051113 ).

                (FlyBase, 1996-)
                Other Comments
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 24 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyMine - An integrated database for Drosophila genomics
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                modMine - A data warehouse for the modENCODE project
                Linkouts
                DRSC - Results frm RNAi screens
                InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                Synonyms and Secondary IDs (8)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (34)