FlyBase Allele Report: Dmel\nej[EP1179]

General Information

Symbol

Dmel\nej^EP1179

Species

D. melanogaster

Name

FlyBase ID

FBal0241474

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

P{EP}nej^EP1179

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Marek et al., 2000)

Mutagen

P-element activity

(Taylor et al., 2003, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Marek et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}nej^EP1179

Cytology

Description

P{EP} element insertion approximately 3kb upstream of the nej open reading frame, in the 5' UTR. The orientation of the P{EP} element is such that the 3' end of the P{EP} element is proximal to the nej open reading frame, allowing overexpression of nej under the control of the Scer\UAS regulatory sequences present in the P{EP} element.

(Marek et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:9,685,850..9,685,850 [-]

(Stofanko et al., 2008, Levine et al., 2007, Singh et al., 2005, Taylor et al., 2003, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Marek et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Jung and Bonini, 2007)

modeled by Hsap\ATXN3^tr.Q46.UAS

(Jung and Bonini, 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

(Stofanko et al., 2008)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PF

(Müller et al., 2005)

neuromuscular junction

(Marek et al., 2000)

neuromuscular junction, with Scer\GAL4^elav.PLu

(Marek et al., 2000)

neuromuscular junction, with Scer\GAL4^Mhc.PW, Scer\GAL4^elav.PLu

(Marek et al., 2000)

neuromuscular junction & bouton

(Marek et al., 2000)

neuromuscular junction & bouton, with Scer\GAL4^elav.PLu

(Marek et al., 2000)

Detailed Description

Statement

Reference

Third instar larvae expressing nej^EP1179 under the control of Scer\GAL4^Pxn.PS have a decreased number of sessile hemocytes. The number of hemocytes in circulation is also decreased. The dorsal sessile hemocyte department is disrupted. Sessile hemocytes accumulate along the dorsal vessel but do not spread through the cuticle. The lymph gland is not enlarged. Most hemocytes appear wild type in shape, although a few larger cells can also be detected.

(Stofanko et al., 2008)

Expression of nej^EP1179 under the control of Scer\GAL4^GMR.PF results in a small eye phenotype.

(Müller et al., 2005)

Expression of CG15321^EP1179 under the control of Scer\GAL4^ey.PH results in eyes that are somewhat reduced in size. Flies expressing CG15321^EP1179 under the control of Scer\GAL4^hs.2sev have glassy eyes.

(Tseng and Hariharan, 2002)

nej^EP1179/nej^TA57 or nej^EP1179/nej^S342 transheterozygotes are semi-viable as third instar larvae. These larvae are developmentally delayed by approximately 1 day and are sluggish. Homozygous larvae show no change in synaptic structure or function compared to wild type. nej^EP1179/nej^TA57 or nej^EP1179/nej^S342 larvae show an approximately 50% decrease in quantal content without any change in quantal size at the neuromuscular junction. No change in the average muscle resting potential or in the average muscle input resistance is seen. Overexpression of nej^EP1179 under the control of Scer\GAL4^elav.PLu (in nej^EP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size. There is a significant decrease in the number of boutons at the neuromuscular junction. Overexpression of nej^EP1179 under the control of both Scer\GAL4^Mhc.PW and Scer\GAL4^elav.PLu (in nej^EP1179 heterozygotes) results in a decrease in presynaptic transmitter release at the neuromuscular junction, without any change quantal size or muscle physiological parameters. Synaptic ultrastructure at neuromuscular boutons appears normal. There is a significant (although moderate) increase in the number of boutons at the neuromuscular junction of muscles 4, 6 and 7 compared to wild type in nej^EP1179/nej^S342, nej^EP1179/nej^Q7 or nej^EP1179/nej^TA57 larvae. No alteration in axon guidance in the embryonic central nervous system is seen.

(Marek et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, nej^EP1179 has visible phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

nej^EP1179, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Scer\GAL4^ey.PH/nej^EP1179 is an enhancer of visible | dominant phenotype of L²

(Singh et al., 2005)

NOT Enhancer of

Statement

Reference

nej^EP1179, Scer\GAL4^{repo.Switch.2301} is a non-enhancer of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

NOT Suppressor of

Statement

Reference

nej^EP1179, Scer\GAL4^{repo.Switch.2301} is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, nej^EP1179 has eye phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

nej^EP1179, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Scer\GAL4^ey.PH/nej^EP1179 is an enhancer of eye phenotype of L²

(Singh et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/nej^EP1179 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Taylor et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of CG15321^EP1179 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

nej^S342

(Marek et al., 2000)

nej^TA57

(Marek et al., 2000)

Comments

nej^EP1179 fails to complement nej^TA57 and nej^S342 for synaptic transmission defects.

(Marek et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30733

w^* P{EP}nej^EP1179

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG15321^EP1179

EP(X)1179

(Abdelilah-Seyfried et al., 2000)

EP¹¹⁷⁹

(Taylor et al., 2003)

dCBP^EP1179

(Jung and Bonini, 2007)

nej-GOF

(Martin et al., 2021)

nej^EP1179

(Alfonso-Gonzalez et al., 2023, Martin et al., 2021, Stofanko et al., 2008)

nej^EP

(Alfonso-Gonzalez et al., 2023)

Name Synonyms

Secondary FlyBase IDs

FBal0104983
FBal0141926

References (14)

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