FlyBase Allele Report: Dmel\Kdm5[10424]

lid¹⁰⁴²⁴/lid^k06801;lid^{JmjC.T:Ivir\HA1} 'rescue' flies display premature transcriptional reactivation as well as precocious karyosome remodeling (the oocytes display early increase in chromatin openness) in prophase I-arrested oocytes and the eggs show impaired entry into embryogenesis after fertilization resulting in strongly reduced female fertility.

(Navarro-Costa et al., 2016)

lid^k06801/lid¹⁰⁴²⁴ mutant female germlines exhibit abnormal morphology of the karyosome, defective clustering of centromeres, and a failure to maintain the synaptonemal complex.

(Zhaunova et al., 2016)

The positional effect variegation (PEV) at the Sb locus caused by T(2;3)Sb^V is significantly enhanced by single copy of lid¹⁰⁴²⁴.

(Dietz et al., 2015)

lid¹⁰⁴²⁴ homozygotes survive at less than 5% rate, whereas about 50% of lid^k06801/lid¹⁰⁴²⁴ mutants survive.

lid^k06801/lid¹⁰⁴²⁴ mutant flies have irregularly shaped mitochondria that are larger than those of wild-type flies and the flies display reduced climbing ability.

(Liu and Secombe, 2015)

lid¹⁰⁴²⁴/Df(2L)BSC184 is pupal lethal, with rare adult escapers. There is a significant decrease of germline stem cell (GSC) number, the majority of GSCs appear to be detached from the hub cells, and average hub area is significantly larger in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes, compared to wild type.

There is a significant decrease in mitotic activity of GSCs (decreased proliferation and premature differentiation) in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes compared to wild type. Spermatogonial cysts containing up to four interconnected germ cells in direct association with the hub region are detected in approximately one third of lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes and differentiated spermatogonial cysts in direct contact with hub cells are detected. No disintegrating fusome remnants are detected in GSCs from lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes and the percentage of GSCs with misoriented centrosomes is not significantly different from wild type.

(Tarayrah et al., 2015)

lid¹⁰⁴²⁴/+ enhances the variegation at the w locus which is seen in the In(1)w^m4h chromosome and enhances the variegation at the mutant Sb locus which is seen in the T(2;3)Sb^V chromosome.

(Di Stefano et al., 2011)

Heterozygous lid¹⁰⁴²⁴ mutants show normal locomotor activity under a 12 hours of light:12 hours of dark (LD) cycle and total activity is equivalent to that of wild type. Under constant darkness (DD) lid¹⁰⁴²⁴/+ flies exhibit disrupted circadian activity rhythms, with the majority of flies showing either no or weak circadian activity rhythms. In flies that do show detectable rhythms, the period is significantly shortened.

(DiTacchio et al., 2011)

Heterozygous flies show strong enhancement of position effect variegation of the w gene caused by In(1)w^m4h.

(Lloret-Llinares et al., 2008)

lid^k06801/lid¹⁰⁴²⁴ animals eclose at approximately half the expected frequency.

(Secombe et al., 2007)

Some homozygotes and lid^k06801/lid¹⁰⁴²⁴ flies appear to die before hatching, although no obvious defects in the larval cuticle are seen. Most homozygotes appear to die at an early pupal stage. Third instar homozygotes often have a small optic brain lobe and small imaginal discs.

(Gildea et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased occurrence of cell division | third instar larval stage phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Tarayrah et al., 2015)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ has partially lethal phenotype, enhanceable by Myc^P0

(Secombe et al., 2007)

Suppressed by

Statement

Reference

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased cell number | third instar larval stage phenotype, suppressible by Ptp61F[+]/Ptp61F^c05292

(Tarayrah et al., 2015)

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased cell number | third instar larval stage phenotype, suppressible by Scer\GAL4^nanos.PU/Stat92E^UAS.cPa

(Tarayrah et al., 2015)

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible by Scer\GAL4^nanos.PU/Stat92E^UAS.cPa

(Tarayrah et al., 2015)

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased cell number | third instar larval stage phenotype, suppressible by shg^UAS.sgGFP/Scer\GAL4^nanos.PU

(Tarayrah et al., 2015)

Kdm5¹⁰⁴²⁴ has enhancer of variegation | dominant phenotype, suppressible by Su(var)3-9¹/Su(var)3-9[+]

(Di Stefano et al., 2011)

NOT suppressed by

Statement

Reference

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by shg^UAS.sgGFP/Scer\GAL4^nanos.PU

(Tarayrah et al., 2015)

Enhancer of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is an enhancer of visible | dominant phenotype of N^Ax-16

(Di Stefano et al., 2011)

NOT Enhancer of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Kdm5¹⁰⁴²⁴ is a non-enhancer of visible phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Suppressor of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Vashishtha et al., 2013)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor of visible | dominant phenotype of Snr1^E1

(Curtis et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of visible phenotype of Su(var)3-3^ΔN

(Di Stefano et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of lethal | recessive | male phenotype of Su(var)3-3^ΔN

(Di Stefano et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor of visible phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Kdm5¹⁰⁴²⁴ is a suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

NOT Suppressor of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Kdm5¹⁰⁴²⁴ is a non-suppressor of visible phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5¹⁰⁴²⁴, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has male germline stem cell | third instar larval stage phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Tarayrah et al., 2015)

Suppressed by

Statement

Reference

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has male germline stem cell | third instar larval stage phenotype, suppressible by Ptp61F[+]/Ptp61F^c05292

(Tarayrah et al., 2015)

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has male germline stem cell | third instar larval stage phenotype, suppressible by Scer\GAL4^nanos.PU/Stat92E^UAS.cPa

(Tarayrah et al., 2015)

Df(2L)BSC184/Kdm5¹⁰⁴²⁴ has male germline stem cell | third instar larval stage phenotype, suppressible by shg^UAS.sgGFP/Scer\GAL4^nanos.PU

(Tarayrah et al., 2015)

Enhancer of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is an enhancer of wing vein phenotype of N^Ax-16

(Di Stefano et al., 2011)

Kdm5¹⁰⁴²⁴ is an enhancer of eye phenotype of CycE^JP

(Brumby et al., 2002)

NOT Enhancer of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Kdm5¹⁰⁴²⁴ is a non-enhancer of eye phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Suppressor of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Vashishtha et al., 2013)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor of wing vein | ectopic phenotype of Snr1^E1

(Curtis et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor of wing vein | ectopic phenotype of Su(var)3-3^ΔN

(Di Stefano et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of wing phenotype of Su(var)3-3^ΔN

(Di Stefano et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor | partially of follicle cell phenotype of Su(var)3-3^ΔN

(Di Stefano et al., 2011)

lid[+]/Kdm5¹⁰⁴²⁴ is a suppressor of eye phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Kdm5¹⁰⁴²⁴ is a suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

NOT Suppressor of

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

lid[+]/Kdm5¹⁰⁴²⁴ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Kdm5¹⁰⁴²⁴ is a non-suppressor of eye phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

lid[+]/Kdm5¹⁰⁴²⁴, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5¹⁰⁴²⁴, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Combination with a single copy of lid¹⁰⁴²⁴ does not modify the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel nor the massive eye overgrowth in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} with Scer\GAL4^GMR.PF or the disrupted wing vein phenotype of flies expressing smo^{5A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^C-765.

(Shalaby et al., 2017)

Stat92E⁰⁶³⁴⁶/+ enhances germline stem cell (GSC) premature differentiation in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes, with spermatocytes found in direct association with hub cells.

Stat92E^Scer\UAS.cPa driven by Scer\GAL4^nos.PU suppresses the GSC loss and premature differentiation (spermatogonial cysts next to the hub) seen in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes.

shg^{Scer\UAS.T:Avic\GFP-rs} driven by Scer\GAL4^nos.PU suppresses the reduced number of germline stem cells (but not the reduced mitotic activity) seen in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes.

Presence of Ptp61F^c05292/+ suppresses the loss of germline stem cells seen in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes.

(Tarayrah et al., 2015)

lid¹⁰⁴²⁴/+ partially suppresses the held-out wing phenotype and largely suppresses the extra wing vein phenotype which is seen in Su(var)3-3^ΔN homozygotes.

lid¹⁰⁴²⁴/+ partially suppresses the follicle cell defects seen in Su(var)3-3^ΔN females.

Su(var)3-9¹/+ suppresses the ability of lid¹⁰⁴²⁴/+ to enhance the variegation at the w locus which is seen in the In(1)w^m4 chromosome.

The wing vein truncation phenotype which is seen in N^Ax-16/+ flies is enhanced by lid¹⁰⁴²⁴/+.

(Di Stefano et al., 2011)

The small, rough eye phenotype caused by expression of asf1^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH is significantly suppressed by lid¹⁰⁴²⁴/+.

(Moshkin et al., 2009)

The dm^P0 ; lid^k06801/lid¹⁰⁴²⁴ combination results in almost complete lethality.

(Secombe et al., 2007)

lid¹⁰⁴²⁴ ash1²² double heterozygotes show T3 to T2 transformations.

(Gildea et al., 2000)

Xenogenetic Interactions

Statement

Reference

The loss of rhabdomere phenotype seen in flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav.PU is partially suppressed by lid¹⁰⁴²⁴/+.

(Vashishtha et al., 2013)

Complementation and Rescue Data

Fails to complement

Kdm5^k06801

(Gildea et al., 2000)

Kdm5^k06801

(BDGP Project Members, 1994-1999)

Rescued by

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^Tag:HA

(Navarro-Costa et al., 2016)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gJmjC.mut

(Zhaunova et al., 2016)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gWT

(Zhaunova et al., 2016)

Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gWT

(DiTacchio et al., 2011)

Kdm5¹⁰⁴²⁴ is rescued by Scer\GAL4^Act.PU/Kdm5^UASp.cSa

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is rescued by Scer\GAL4^Act.PU/Kdm5^C1296A.UASp

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gWT

(Li et al., 2010)

Partially rescued by

Kdm5¹⁰⁴²⁴ is partially rescued by Kdm5^gJmjC.mut

(DiTacchio et al., 2011)

Kdm5¹⁰⁴²⁴ is partially rescued by Scer\GAL4^Act.PU/Kdm5^ΔPHD1.UASp

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is partially rescued by Scer\GAL4^Act.PU/Kdm5^ΔC5HC2.UASp

(Li et al., 2010)

Not rescued by

Kdm5¹⁰⁴²⁴ is not rescued by Scer\GAL4^Act.PU/Kdm5^{JmjC.mut.UASp}

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is not rescued by Scer\GAL4^Act.PU/Kdm5^ΔJmjN.UASp

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is not rescued by Scer\GAL4^Act.PU/Kdm5^ΔARID.UASp

(Li et al., 2010)

Kdm5¹⁰⁴²⁴ is not rescued by Kdm5^ΔPHD3.UASp/Scer\GAL4^Act.PU

(Li et al., 2010)

Comments

The premature transcriptional reactivation as well as precocious karyosome remodeling (the oocytes display early increase in chromatin openness) in prophase I-arrested lid¹⁰⁴²⁴/lid^k06801 transheterozygous oocytes is rescued by combination with lid^T:Ivir\HA1.

(Navarro-Costa et al., 2016)

Expression of lid^+mDa rescues the locomotor rhythm defects seen in homozygous lid¹⁰⁴²⁴ mutant flies.

Expression of lid^gJmjC.mut partially rescues the locomotor rhythm defects seen in homozygous lid¹⁰⁴²⁴ mutant flies. Under LD (12 hours of light:12 hours of dark) and DD conditions (constant darkness) flies exhibit attenuated day:night differences in activity, with flies showing increased activity around midday and reduced dark anticipation.

(DiTacchio et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12367

cn¹ P{PZ}Kdm5¹⁰⁴²⁴/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Precise excision of the P{PZ} element reverts the lid¹⁰⁴²⁴ phenotype.

(Gildea et al., 2000)

Complements: eIF-4a⁰²⁴³⁹. Complements: eIF-4a^k01801. Complements: eIF-4a^k07120. Complements: eIF-4a^k09319a. Complements: chic^k13321. Complements: eIF-4a^k14518. Complements: eIF-4a^k14610. Complements: eIF-4a^k16006.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Kdm5¹⁰⁴²⁴

(Herz and Bergmann, 2024)

Lid¹⁰⁴²⁴

(Lee and Spradling, 2014)

dkdm5¹⁰⁴²⁴

(Navarro-Costa et al., 2016)

kdm5¹⁰⁴²⁴

(Han et al., 2023, Chen et al., 2019, Drelon et al., 2018, Liu and Secombe, 2015, Liu et al., 2014)

l(2)10424

(Secombe et al., 2007, Gildea et al., 2000, Spradling et al., 1999)

l(2)10424¹⁰⁴²⁴

(BDGP Project Members, 1994-1999)

lid¹⁰⁴²⁴

(Shalaby et al., 2018, Shalaby et al., 2017, Hong and Choi, 2016, Zhaunova et al., 2016, Tarayrah et al., 2015, Liu et al., 2014, Debruhl et al., 2013, Ji et al., 2012, Di Stefano et al., 2011, DiTacchio et al., 2011, Li et al., 2010, Moshkin et al., 2009, Secombe et al., 2007)

lid¹²³⁶⁷

(Lloret-Llinares et al., 2008)

lid¹

(Curtis et al., 2011, Brumby et al., 2002, Gildea et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (30)

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