FlyBase Allele Report: Dmel\nanos[L7]

General Information

Symbol

Dmel\nanos^L7

Species

D. melanogaster

Name

FlyBase ID

FBal0013153

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

Also Known As

nos^L7

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Kraut and Levine, 1991)

loss of function allele

(Eldon and Pirrotta, 1991)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Deletes nucleotides 2009-2029, inclusive, eliminating seven codons (ITMEDAI). These seven residues fall just outside the second CCHC domain.

(Curtis et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:19,159,242..19,159,262 [+]

Comment:

Deletion of seven codons (ITMEDAI).

(Curtis et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | maternal effect | recessive

(Lehmann and Nusslein-Volhard, 1991)

Phenotype Manifest In

abdomen

(Wang and Lehmann, 1991, Lehmann and Frohnhofer, 1989)

abdominal segment

(Curtis et al., 1995)

embryonic/first instar larval cuticle

(Cockerill et al., 1993)

embryonic abdominal segment

(Bajusz et al., 2001)

embryonic abdominal segment 1

(Lehmann, 1988)

embryonic abdominal segment 2

(Lehmann, 1988)

embryonic abdominal segment 3

(Lehmann, 1988)

embryonic abdominal segment 4

(Lehmann, 1988)

embryonic abdominal segment 5

(Lehmann, 1988)

embryonic abdominal segment 6

(Lehmann, 1988)

embryonic abdominal segment 7

(Lehmann, 1988)

embryonic abdominal segment 8

(Lehmann, 1988)

embryonic abdominal segment 9

(Lehmann, 1988)

embryonic abdominal segment 10

(Lehmann, 1988)

embryonic abdominal segment 11

(Lehmann, 1988)

germline cell

(Heller and Steinmann-Zwicky, 1998)

mitotic domain 1 | extended germ band stage

(Irvine and Wieschaus, 1994)

Detailed Description

Statement

Reference

Embryos derived from homozygous females have less than 3 abdominal segments.

(Bajusz et al., 2001)

Embryos form a wildtype number of germ cells at blastoderm but not all of theses germ cells enter the mutant embryos. Cells that remain outside degenerate.

(Heller and Steinmann-Zwicky, 1998)

Germ cells are present in embryos derived from homozygous females.

(Heller and Steinmann-Zwicky, 1998)

Has no effect on the posterior accumulation of G-iα65A protein.

(Wolfgang and Forte, 1995)

bcd, nos double mutants show reduced rate of germ band extension and cell intercalation.

(Irvine and Wieschaus, 1994)

Embryos from homozygous mothers produce extra pole cells at the posterior pole.

(Bardsley et al., 1993)

Embryos derived from homozygous mothers lack abdominal cuticle. 22% of embryos carrying nub^hs.PC and derived from homozygous mothers produce 2-3 abdominal denticle belts when heat shocked at 1.75 to 2.75 hours of development. 4% of embryos carrying pdm2^hs.PC and derived from homozygous mothers produce 2-3 abdominal denticle belts when heat shocked at 1.75 to 2.75 hours of development.

(Cockerill et al., 1993)

Embryos carrying six copies of osk (6xP{osk⁺108}) examined at the cellular blastoderm stage exhibit ectopic pole cell formation, this ectopic formation is enhanced in a nos^L7 mutant background.

(Smith et al., 1992)

Uniform distribution of maternal hb protein. The posterior gt stripe fails to appear, while the anterior pattern appears normal.

(Eldon and Pirrotta, 1991)

Strong abdominal defects.

(Ephrussi et al., 1991)

Posterior gt domain is usually absent.

(Kraut and Levine, 1991)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Homozygous embryos lack all abdominal segments. Extreme embryos exhibit a bic phenotype.

(Wang and Lehmann, 1991)

Large amounts of vas protein are expressed in early stages of oogenesis. vas protein localizes to the posterior pole at the same stage of egg development as wild type.

(Lasko and Ashburner, 1990)

nos^L7/nos⁵³ transheterozygous embryos derived from homozygous females have polar granules and pole cells and have deletions of abdominal structures. CycB and vas transcript localization and accumulation is as in wild type.

(Raff et al., 1990)

Abdominal segments deleted, head, thorax and telson are normal. Posterior transplantation of anterior cytoplasm from wild type embryos induced high rate of anterior structure duplication.

(Sugiyama and Okada, 1990)

No abdominal segmentation is formed but head, thorax and telson are normal. Injection of wild type posterior pole plasm induces formation of an almost complete set of abdominal segments in normal anteroposterior orientation.

(Lehmann and Frohnhofer, 1989)

All abdominal segments are deleted in embryos derived from homozygous females.

(Lehmann, 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a suppressor | partially of abnormal size | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of increased cell number | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Df(3R)Exel6183/nanos^L7 is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^en.PU, yki^UAS.cHa

(Zhang et al., 2015)

NOT Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a non-suppressor | partially of abnormal size | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of increased cell number | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Other

Statement

Reference

E2f2^JF02828, Scer\GAL4^nub.PU, nanos^L7 has visible | adult stage phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, mip130^HMS00462, nanos^L7 has visible | adult stage phenotype

(Miles et al., 2014)

bcd⁶, nanos^L7, tsl³ has abnormal cell polarity | maternal effect | gastrula stage phenotype

(Zallen and Wieschaus, 2004)

hb¹⁵, nanos^L7 has viable phenotype

(Arrizabalaga and Lehmann, 1999)

hb¹⁵, nanos^L7 has fertile phenotype

(Arrizabalaga and Lehmann, 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

nanos^L7 has embryonic abdominal segment phenotype, suppressible by hb⁴

(Bajusz et al., 2001)

hb⁴, nanos^L7 has embryonic abdominal segment phenotype, suppressible by Df(2R)vg-B

(Saget et al., 1998)

hb⁴, nanos^L7 has embryonic abdominal segment phenotype, suppressible | partially by pho^b

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal tergite phenotype, suppressible by mxc^G48

(Saget et al., 1998)

hb⁴, nanos^L7 has embryonic abdominal segment phenotype, suppressible by mxc^G48

(Saget et al., 1998)

nanos^L7 has phenotype, suppressible by Dvir\nanos^tCa

(Curtis et al., 1995)

Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a suppressor | partially of egg chamber phenotype of lky^KO

(Antel et al., 2022)

nanos^BN/nanos^L7 is a suppressor | partially of ovary phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of oocyte | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Df(3R)Exel6183/nanos^L7 is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en.PU, yki^UAS.cHa

(Zhang et al., 2015)

NOT Suppressor of

Statement

Reference

nanos^L7/nos[+] is a non-suppressor of ovary phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of oocyte | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^L7/nos[+] is a non-suppressor of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Other

Statement

Reference

E2f2^JF02828, Scer\GAL4^nub.PU, nanos^L7 has wing vein | ectopic phenotype

(Miles et al., 2014)

E2f2^JF02828, Scer\GAL4^nub.PU, nanos^L7 has wing blade phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, mip130^HMS00462, nanos^L7 has wing vein | ectopic phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, mip130^HMS00462, nanos^L7 has wing blade phenotype

(Miles et al., 2014)

bcd⁶, nanos^L7, tsl³ has embryo | embryonic stage 4 phenotype

(Blankenship and Wieschaus, 2001)

hb¹⁵, nanos^L7 has germline cell phenotype

(Arrizabalaga and Lehmann, 1999)

apt¹⁶⁷, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

apt⁴¹, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

apt^tdf-PΔ3, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

apt^tdf-PΔ4, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

hb⁴, nanos^L7 has embryonic abdominal segment phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 4 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 5 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 8 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal tergite phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 1 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 2 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 3 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 6 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, nanos^L7 has abdominal 7 ventral denticle belt phenotype

(Saget et al., 1998)

hb⁴, mxc^G48, nanos^L7 has abdominal tergite phenotype

(Saget et al., 1998)

bcd⁶, nanos^L7 has telson phenotype

(Lehmann and Frohnhofer, 1989)

bcd⁶, nanos^L7, tll¹ has epidermis phenotype

(Lehmann and Frohnhofer, 1989)

bcd⁶, nanos^L7, tll¹ has telson phenotype

(Lehmann and Frohnhofer, 1989)

bcd⁶, nanos^L7, tsl³ has epidermis phenotype

(Lehmann and Frohnhofer, 1989)

Additional Comments

Genetic Interactions

Statement

Reference

nos^L7/Df(3R)Exel6183 partially suppresses the wing disc posterior compartment overgrowth of larvae expressing yki^Scer\UAS.cHa under the control of Scer\GAL4^en.PU.

(Zhang et al., 2015)

Expression of either E2f2^JF02828 or mip130^HMS00462 under the control of Scer\GAL4^nub.PU (using UAS-Dicer2 to enhance RNAi efficiency) in the nos^L7 mutant background results in adult wing defects (minor ectopic venation). No wing phenotype is observed upon expression of either Rbf^HMS03004 or mip120^HMS00461 in the nos^L7 mutants.

(Miles et al., 2014)

Anisotropic epithelial constriction still occurs in bcd⁶ nos^L7 tsl³ triple mutant embryos that lack anterior-posterior polarity.

(Martin et al., 2010)

wah¹, nos^BN/nos^L7 females do not exhibit age-dependent loss of fecundity.

(Yu et al., 2010)

Anterior-posterior polarization of cells in the extending germband is disrupted in embryos from bcd⁶ nos^L7 tsl³ homozygous mothers.

(Zallen and Wieschaus, 2004)

3.69% of embryos derived from hb⁴ nos^L7/nos^L7 females have at least 3 abdominal segments.

(Bajusz et al., 2001)

Embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females have uniform yolk stalk diameters (in contrast to wild type where there are three domains of varying yolk stalk diameter along the anterior-posterior axis of the embryo). The shallow cellularisation front of the anterior domain and the greater depth of the pre-cephalic furrow domain are also lost. Other aspects of cellularisation are normal in these embryos. Nuclear spacing is uniform along the anterior-posterior axis in embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females (in contrast to wild type where there is an anterior domain of lower nuclear density). The larger diameter of the actin caps seen in the anterior of the wild-type embryo in cycle 11 and 12 is not seen in embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females.

(Blankenship and Wieschaus, 2001)

nos^L7 homozygous embryos derived from nos^L7 hb¹⁵ homozygous female germline clones form germ cells normally, but show defects after stage 10 of embryogenesis. At this stage, many germ cells fail to leave the gut (in contrast to wild type) and tightly associate into clusters of cells. Some germ cells follow a normal migratory pattern, the morphology of the germ cells is normal and the embryos develop into fertile flies.

(Arrizabalaga and Lehmann, 1999)

Embryos derived from hb⁴ nos^L7/nos^L7 females differentiate only a few abdominal denticle belts. No rescue of abdominal segments is seen if the females also carry Df(1)10-70d, Df(1)mxc-1, mxc^22a-6 or mxc¹. Rescue of abdominal segments in embryos derived from hb⁴ nos^L7/nos^L7 females is seen if the females are also carrying mxc^G48. Adult escapers have abnormal tergites and are mostly fertile. Rescue of abdominal segments in embryos derived from hb⁴ nos^L7/nos^L7 females is seen if the females are also carrying Df(2R)vg-B or pho^b.

(Saget et al., 1998)

bcd⁶ nos^L7 embryos exhibit two telsons in mirror image. Injection of wild type posterior pole plasm induces formation of two mirror image abdomens. bcd⁶ nos^L7 tll¹ embryos exhibit two telsons in mirror image. Injection of wild type posterior pole plasm induces formation of two mirror image abdomens. bcd⁶ nos^L7 tsl³ embryos develop a cuticle but no segmental pattern. Injection of wild type posterior pole plasm induces formation of anterior abdominal segments towards the end of the embryo.

(Lehmann and Frohnhofer, 1989)

Xenogenetic Interactions

Statement

Reference

Two copies of P{Dvnos} partially rescues the mutant phenotype.

(Curtis et al., 1995)

Complementation and Rescue Data

Rescued by

nanos^L7 is rescued by nanos^+4.4.Tag:HA

(Curtis et al., 1995)

Not rescued by

nanos^L7 is not rescued by nanos^{108.αTub84B.UTR.Tag:HA}

(Arrizabalaga and Lehmann, 1999)

Comments

One copy of P{Dmnos} is sufficient to completely rescue the nos^L7 abdominal segmentation phenotype.

(Curtis et al., 1995)

Abdominal segmentation defect can be rescued by injection of nos^N5 RNA before pole cell formation, embryos hatch and develop into fertile adults. Injection into the head region causes an anterior shift in the postion of the cephalic furrow and reduction of head skeletal structures.

(Wang and Lehmann, 1991)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

3285

st¹ nanos^L7 e¹/TM3, Sb¹ Ser¹

Kyoto

106942

st¹ pum⁶⁸⁰ nanos^L7 e¹

106957

st¹ nanos^L7 e¹/TM3, Sb¹

106945

st¹ kni^ri-1 bcd⁶ rn^roe-1 p^p nanos^L7 e¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Lehmann.

Comments

hb protein expression in early nos^L7 mutant embryos has been studied.

(Tautz, 1988)

Posterior cytoplasm from embryos derived from nos^L7 females can rescue both anterior and posterior defects when transplanted into the anterior or posterior end respectively of embryos derived from tor⁴ females.

(Sugiyama and Okada, 1990)

Pole plasm is present and functional in germ cell determination. Cytoplasmic transplantation of wild type pole plasm into the abdominal region restores normal abdominal development: surviving adults are fertile.

(Lehmann and Nusslein-Volhard, 1991)

The posterior localization of tud product is unaffected in mutant embryos.

(Bardsley et al., 1993)

Cytoplasm from the posterior pole of D.pseudoobscura.pseudoobscura, D.virilis, D.hydei, M.domestica and C.samoensis embryos is injected into the abdominal region of homozygous early cleavage stage embryos. Injected embryos displayed partial or complete abdominal segmentation, the frequency of strong rescue decreases with increasing evolutionary distance of the donor.

(Curtis et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nanos^L7

nos^L7

(Antel et al., 2022, Coux et al., 2018, Wharton et al., 2018, Suresh et al., 2017, Zhang et al., 2015, Miles et al., 2014, Yu et al., 2010, Sofola et al., 2007, Tadros et al., 2007)

nos^l7

(Singh et al., 2022, Hannon et al., 2017, Zhang et al., 2015)

Name Synonyms

nanosL7

(Martin et al., 2010)

Secondary FlyBase IDs

References (73)

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