FlyBase Allele Report: Dmel\cno[mis1]

General Information

Symbol

Dmel\cno^mis1

Species

D. melanogaster

Name

misty 1

FlyBase ID

FBal0042918

Feature type

allele

Associated gene

Dmel\cno

Associated Insertion(s)

P{BmΔ-w}cno^mis1

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Matsuo et al., 1997)

Mutagen

Delta2-3 transposase

(Miyamoto et al., 1995)

Progenitor genotype

Associated Insertion(s)

P{BmΔ-w}cno^mis1

Cytology

Description

P{BmΔ-w} insertion 442bp upstream of the tentative cno translation start site.

(Miyamoto et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage (with cno²)

(Boettner and Van Aelst, 2007)

abnormal cell polarity

(Gaengel and Mlodzik, 2003)

abnormal cell polarity (with cno²)

(Gaengel and Mlodzik, 2003)

some die during embryonic stage (with cno²)

(Boettner and Van Aelst, 2007, Boettner et al., 2003)

visible

(Miyamoto et al., 1995)

visible (with cno²)

(Chen et al., 2000)

visible | recessive

(Chen et al., 2000)

Phenotype Manifest In

cone cell

(Matsuo et al., 1997, Miyamoto et al., 1995)

ectoderm | embryonic stage (with cno²)

(Boettner and Van Aelst, 2007)

embryo (with cno²)

(Boettner and Van Aelst, 2007)

embryo | dorsal closure stage (with cno²)

(Boettner et al., 2003)

embryo | embryonic stage 14 (with cno²)

(Boettner et al., 2003)

embryonic/first instar larval cuticle | embryonic stage (with cno²)

(Boettner and Van Aelst, 2007)

eye

(Chen et al., 2000)

eye photoreceptor cell

(Matsuo et al., 1997)

eye photoreceptor cell (with cno²)

(Chen et al., 2000)

macrochaeta & postpronotum

(Miyamoto et al., 1995)

macrochaeta & scutellum

(Miyamoto et al., 1995)

macrochaeta & scutum

(Miyamoto et al., 1995)

macrochaeta & wing

(Miyamoto et al., 1995)

ommatidium

(Gaengel and Mlodzik, 2003, Chen et al., 2000, Matsuo et al., 1997, Miyamoto et al., 1995)

ommatidium (with cno²)

(Fetting et al., 2009, Gaengel and Mlodzik, 2003, Chen et al., 2000)

photoreceptor cell R7

(Matsuo et al., 1999)

photoreceptor cell R8

(Matsuo et al., 1999)

pigment cell

(Matsuo et al., 1997)

primary pigment cell

(Miyamoto et al., 1995)

retina

(Chen et al., 2000)

rhabdomere

(Matsuo et al., 1999, Matsuo et al., 1997)

rhabdomere R1 & microvillus

(Matsuo et al., 1999)

rhabdomere R2

(Matsuo et al., 1999)

rhabdomere R3

(Matsuo et al., 1999)

rhabdomere R4 & microvillus

(Matsuo et al., 1999)

Detailed Description

Statement

Reference

Many cno²/cno^mis1 ommatidia mis-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. Most of the ommatidia over rotate.

(Fetting et al., 2009)

54% of cno²/cno^mis1 embryos hatch, 23% have their cuticle closed dorsally (with 16% having a head involution defect), 22% have the cuticle partially open and 1% have a large dorsal hole in the cuticle (covering at least half of the dorsal aspect).

(Boettner et al., 2003)

Homozygous mutants and cno^mis1/cno² animals exhibit an ommatidial rotation phenotype in the adult eye which is also seen in third instar eye imaginal discs.

(Gaengel and Mlodzik, 2003)

Homozygotes have an extremely rough eye phenotype and the retinas are quite disorganised. cno^mis1/cno² flies have mostly normal facets in the eye, with a small proportion of facets missing photoreceptors.

(Chen et al., 2000)

Photoreceptor rhabdomeres appear twisted, branched and fused to each other in homozygous flies. Some rhabdomeres do not reach the apical surface of the retina. A considerable fraction of the rhabdomeres fall beneath the fenestrated membrane (which in wild type forms a clear boundary between the retina and brain). The positions of the photoreceptors are aberrant in homozygous ommatidia; the R7 and R8 photoreceptors appear in the same tangential cross section in mutant flies (this is never seen in wild type). The rhabdomeres of R2 and R3 are fused to each other, although the boundary between them can still be seen. The arrangement of microvilli is normal in all rhabdomeres except the R1 and R4 rhabdomeres which show a slight disturbance.

(Matsuo et al., 1999)

Homozygous embryos develop normally. cno^mis1/cno³ embryos have no apparent defects in dorsal closure.

(Takahashi et al., 1998)

Rough eye phenotype; ommatidia have variable size and shape (rhabdomeres are of variable number and have distorted shapes in unusual positions in the ommatidia), ommatidial fusions are also exhibited. In pupal retina the alignment of ommatidial rows is disrupted, photoreceptors occupy unusual positions probably because the mutant discs fail to form a correct mesh of pigment cells that shape ommatidia into regular hexagonal structures. Ommatidia may also have an additional cone cell or be missing 1-3 cone cells.

(Matsuo et al., 1997)

Homozygotes display a severe rough eye phenotype, facets have irregular size and shape and two or three adjacent ommatidia are fused. Ommatidia have irregular number of cone cells and extra primary pigment cells. Some homozygotes have extra macrochaetae on the head, notum, humerus and/or scutellum. Hemizygotes show wing phenotypes of a notched blade and loss of crossvein. Transheterozygotes cno^mis1/cno² and cno^mis1/cno³ have a roughened eye structure.

(Miyamoto et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has some die during embryonic stage phenotype, enhanceable by Rap1^CD5/Rap1^CD5

(Boettner et al., 2003)

Enhancer of

Statement

Reference

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

Other

Statement

Reference

cno^mis1/cno², zip^Ebr has visible | adult stage phenotype

(Boettner and Van Aelst, 2007)

cno^mis1, pyd^tam has lethal | dominant phenotype

(Takahashi et al., 1998)

cno^mis1, mys⁸ has partially lethal phenotype

(Miyamoto et al., 1995)

cno^mis1, sgg³² has visible phenotype

(Miyamoto et al., 1995)

Phenotype Manifest In

Enhanced by

Statement

Reference

cno^mis1/cno² has ommatidium phenotype, enhanceable by ed[+]/ed^1X5

(Fetting et al., 2009)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo | dorsal closure stage phenotype, enhanceable by Rap1^CD5/Rap1^CD5

(Boettner et al., 2003)

NOT Enhanced by

Statement

Reference

cno^mis1 has ommatidium phenotype, non-enhanceable by faf^FO8/faf[+]

(Chen et al., 2000)

cno^mis1/cno² has ommatidium phenotype, non-enhanceable by faf^FO8/faf[+]

(Chen et al., 2000)

cno^mis1 has phenotype, non-enhanceable by a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Suppressed by

Statement

Reference

cno^mis1/cno² has ommatidium phenotype, suppressible by fred[+]/fred^H24

(Fetting et al., 2009)

NOT suppressed by

Statement

Reference

cno^mis1 has phenotype, non-suppressible by a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Enhancer of

Statement

Reference

cno^mis1/cno² is an enhancer of embryo phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of embryo phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

NOT Enhancer of

Statement

Reference

cno^mis1/cno^mis1 is a non-enhancer of phenotype of a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Suppressor of

Statement

Reference

cno^mis1 is a suppressor of wing vein phenotype of N^Ax-1

(Miyamoto et al., 1995)

NOT Suppressor of

Statement

Reference

cno^mis1/cno^mis1 is a non-suppressor of phenotype of a⁷/Df(2R)a^EX1

(Liu and Lengyel, 2000)

Other

Statement

Reference

cno^mis1/cno², zip^Ebr has wing phenotype

(Boettner and Van Aelst, 2007)

cno^mis1, pyd^tam/pyd[+] has embryonic/first instar larval cuticle | dorsal phenotype

(Takahashi et al., 1998)

cno^mis1/cno³, hep¹ has embryonic/first instar larval cuticle | dorsal phenotype

(Takahashi et al., 1998)

cno^mis1/cno[+], pyd^tam has embryonic/first instar larval cuticle phenotype

(Takahashi et al., 1998)

bsk², cno^mis1/cno³ has embryonic/first instar larval cuticle | dorsal phenotype

(Takahashi et al., 1998)

cno^mis1/cno³, hep[+]/hep¹ has embryonic/first instar larval cuticle | dorsal phenotype

(Takahashi et al., 1998)

bsk²/bsk[+], cno^mis1/cno³ has embryonic/first instar larval cuticle | dorsal phenotype

(Takahashi et al., 1998)

N^spl-1, cno^mis1 has macrochaeta phenotype

(Miyamoto et al., 1995)

cno^mis1, mys¹¹ has wing vein phenotype

(Miyamoto et al., 1995)

cno^mis1, mys⁸ has wing vein phenotype

(Miyamoto et al., 1995)

N^spl-1, cno^mis1 has wing phenotype

(Miyamoto et al., 1995)

cno^mis1, sca¹ has wing phenotype

(Miyamoto et al., 1995)

cno^mis1, sca¹ has ommatidium phenotype

(Miyamoto et al., 1995)

N^spl-1, cno^mis1 has ommatidium phenotype

(Miyamoto et al., 1995)

cno^mis1, sgg³² has wing vein phenotype

(Miyamoto et al., 1995)

cno^mis1, sca¹ has macrochaeta phenotype

(Miyamoto et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

ed^1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in cno²/cno^mis1 animals.

fred^H24 dominantly suppresses the phenotype of mis-rotation of ommatidia that is seen in cno²/cno^mis1 animals.

(Fetting et al., 2009)

Homozygosity for R^CD5 enhances the cno²/cno^mis1 phenotype; only 4% of cno^mis1 R^CD5/cno² R^CD5 double mutant embryos develop into larvae or have a completely closed cuticle, 37% have large anterior holes in the cuticle and 59% are completely open dorsally.

(Boettner et al., 2003)

The addition of cno^mis1/cno^mis1 has no effect on a⁷/Df(2R)a^EX1 flies.

(Liu and Lengyel, 2000)

hep¹ and bsk² dominantly enhance the cno^mis1/cno³ phenotype; hep¹/+; cno^mis1/cno³ and bsk²/+; cno^mis1/cno³ embryos have a dorsal open phenotype. pyd^tam cno^mis1 double heterozygotes die as embryos with a dorsal open phenotype. The leading edge cells show little elongation during stage 15 (at or slightly before completion of dorsal closure).

(Takahashi et al., 1998)

Double mutants with sca¹ and with N^spl-1 always have rumpled wings curved downwards, rough eye phenotype and extra bristles. cno^mis1 causes dominant suppression of N^Ax-1 wing vein gaps, mutant phenotype is fully recovered in cno^mis1 homozygotes. Individuals in combination with mys¹¹ exhibit incomplete lethality, few escapers have normal wings or an extra wing vein phenotype.

(Miyamoto et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Kyoto

109031

w^*; P{BmΔ-w}cno^mis1 / TM3

109030

P{BmΔ-w}cno^mis1 P{BmΔ-w}pyd^tam / TM3, P{ftz-lacZ.ry⁺}TM3, Sb¹ ry^*

Notes on Origin

Discoverer

Comments

Excision of the P{BmΔ-w} element reverts the mutant phenotype to wild type.

(Miyamoto et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

canoe^mis1

(Matsuo et al., 1999)

cno^Mis1

(Gaengel and Mlodzik, 2003)

cno^mis1

(Fetting et al., 2009, Boettner and Van Aelst, 2007)

cno^misty1

(Matsuo et al., 1995)

mis¹

Name Synonyms

misty 1

(Miyamoto et al., 1995)

Secondary FlyBase IDs

References (11)

Report Sections

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