The clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU does not induce ectopic polar/stalk cells on the main body region in stage 4 egg chambers.
The expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood, leads to an increase in the number of basal stalk cells, associated with basal stalk defects, as compared to controls.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4ato.3.6 strongly reduces the number of medulla axons in the adult brain compared to wild type, while the initial outgrowth of these axons towards the medulla (at 32 hours after puparium formation) is not inhibited.
Type II neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4wor.PA contain mostly supernumerary neuroblasts and a few immature intermediate neural progenitor cells when analysed 72 hours after clone induction.
Type I neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of six neuroblasts per clone, although only 60% of the clones contain more than one neuroblast per clone.
Tracheal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4btl.PS prevents tracheal fusion, but does not arrest outgrowth or stall cells near the base of the ganglionic branch.
Expression of Nintra.GS.Scer\UAS in the eye disc under the control of Scer\GAL4Act5C.PP results in clones that overproliferate compared to wild type cells.
Expression of Nintra.GS.Scer\UAS using Scer\GAL4CG31670.R9D11 transforms Intermediate Neural Progenitors into ectopic type II neuroblasts in larval brains.
Follicle stem cell clones expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4tub, Scer\GAL4Act and Scer\GAL4e22c simultaneously are lost more rapidly from the ovarioles than control clones over time.
Follicle stem cell clones expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4tub are lost more rapidly from the ovarioles than control clones over time.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4C587 results in an increased number of cap cells in the female germarium. Extra cap cells are seen both at the normal location close to the terminal filament cells and also in ectopic locations away from the germarial tip. In germaria with increased numbers of cap cells in the normal location, there is also an increase in the number of spectrosome-containing single germ cells, which behave like germline stem cells (GSCs), at the germarial tip. The ectopically located cap cells appear to be functional as spectrosome-containing single germ cells, which behave as GSCs, are also closely associated with them. These ectopic GSCs anchor their spectrosome on the side that anchors the ectopic cap cell, as occurs in a normal GSC context. Some of the ectopic cap cells are surrounded by inner germarial sheath (IGS) cells or somatic follicle cells. Germ cells lying one cell away from the ectopic GSCs are often germline cysts (as they contain branched fusomes), indicating that the progeny of the ectopic GSCs can probably undergo normal differentiation. However, too many cap cells (more than seven) at either the normal or ectopic location often cause the accumulation of spectrosome-containing single germ cells (which resemble GSCs) located two or more cell diameters away.
When Nintra.GS.Scer\UAS is expressed under the control of Scer\GAL4Act5C.PI in inner germarial sheath cells of the adult ovary does not affect the differentiation status of the underlying germ cells.
Nintra.GS.Scer\UAS; Scer\GAL4fkh.14-3 causes ectopic cell movements in the developing proventriculus, but no obvious changes in endodermal or ectodermal cell fate.
Nintra.GS.Scer\UAS; Scer\GAL4dpp.blk1 results in overgrowth of the wing pouch and notum and an increase in BrdU incorporation in both of these regions. Similar results are seen in Nintra.GS.Scer\UAS; Scer\GAL4αTub84B.PL somatic clones. These clones also induce overgrowth non-autonomously. Flow cytometry analysis of cell cycle progression in the mutant cells shows no difference in profile compared to wild-type cells.
Nintra.GS.Scer\UAS; Scer\GAL4pros.PMG 30% of RP2 neurons are transformed into RP2 sib neurons, 99% of dMP2 neurons are converted to vMP2, 92% of hemi-segments exhibit an increase in U neurons and there is am overall decrease in EL neurons.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PI results in the formation of ectopic polar cells near the two poles of the egg chambers.
Embryos expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4arm.PS show loss of peripheral nervous system neurons and the central nervous system is reduced to irregular patches of tissue.
When Nintra.GS.Scer\UAS is driven by Scer\GAL4hs.PB and heatshocked between 8-12 hours APF no effect is seen on bristle pattern, but a high number of bristles lack bracts. Heat shock at earlier stages strongly reduces the number of bristles. Heatshock at later stages, causes many bristles to lack both shaft and bract.
Nintra.GS.Scer\UAS when driven by Scer\GAL4F442A.αTub84B.T:Hsim\VP16 suppresses neuroblast segregation in the embryo.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4ato.3.6 results in a severe inhibition of axonal branching over the lobula and a complete failure of innervation of the medulla.
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has increased cell number | larval stage phenotype, enhanceable by Df(2L)erm2/+
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has increased cell number | larval stage phenotype, enhanceable by Df(2L)Exel7071/+
Nintra.GS.UAS, Scer\GAL4Act5C.PP has abnormal neuroanatomy | larval stage | somatic clone phenotype, enhanceable | somatic clone by kluUAS.Tag:HA, Scer\GAL4Act5C.PP
Nintra.GS.UAS, Scer\GAL4eg-Mz360 has decreased cell number | larval stage phenotype, non-enhanceable by hbUAS.cWa, Scer\GAL4eg-Mz360
Nintra.GS.UAS, Scer\GAL4wor.PA has abnormal neuroanatomy | larval stage phenotype, suppressible by kluunspecified/kluunspecified
Nintra.GS.UAS, Scer\GAL4Act5C.PP has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible by kluunspecified/kluunspecified
Nintra.GS.UAS, Scer\GAL4GMR9D11 has abnormal neuroanatomy phenotype, suppressible by ermUAS.Tag:HA, Scer\GAL4GMR9D11
Nintra.GS.UAS, Scer\GAL4eg-Mz360 has decreased cell number | larval stage phenotype, non-suppressible by hbUAS.cWa, Scer\GAL4eg-Mz360
Nintra.GS.UAS, Scer\GAL4109-30 has increased cell number | oogenesis phenotype, non-suppressible by Six4HM05254, Scer\GAL4109-30
Nintra.GS.UAS, Scer\GAL4Act5C.PP has increased cell number | somatic clone | larval stage phenotype, non-suppressible by Vha55j2E9
Nintra.GS.UAS has abnormal size | somatic clone | larval stage phenotype, non-suppressible by Vha55j2E9
Nintra.GS.UAS, Scer\GAL4Bx-MS1096 is an enhancer of increased size | larval stage phenotype of NkapGD11807, Scer\GAL4Bx-MS1096
Scer\GAL4Act.PU/Nintra.GS.UAS is an enhancer of increased cell number | oogenesis | somatic clone phenotype of AxnS044230
Scer\GAL4GMR45D11/Nintra.GS.UAS is a suppressor | partially of abnormal cell number | oogenesis phenotype of RpS5bCRISPR
Scer\GAL4Act5C.PP/Nintra.GS.UAS is a suppressor of decreased cell number | somatic clone | larval stage phenotype of Vha55j2E9
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has increased cell number | larval stage phenotype
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has increased cell number | larval stage phenotype
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has type II neuroblast | larval stage | increased number phenotype, enhanceable by Df(2L)erm2/+
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has larval brain | larval stage phenotype, enhanceable by Df(2L)erm2/+
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has type II neuroblast | larval stage | increased number phenotype, enhanceable by Df(2L)Exel7071/+
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has larval brain | larval stage phenotype, enhanceable by Df(2L)Exel7071/+
Nintra.GS.UAS, Scer\GAL4Act5C.PP has type I neuroblast | larval stage | increased number | somatic clone phenotype, enhanceable | somatic clone by kluUAS.Tag:HA, Scer\GAL4Act5C.PP
Nintra.GS.UAS, Scer\GAL4eg-Mz360 has larval ventral nerve cord phenotype, non-enhanceable by hbUAS.cWa, Scer\GAL4eg-Mz360
Nintra.GS.UAS, Scer\GAL4pros.PMG has larval EL neuron phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has larval RP2 motor neuron phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has RP2sib neuron | increased number phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has U neuron | increased number phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has Usib neuron phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has dMP2 neuron phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has vMP2 neuron | increased number phenotype, non-enhanceable by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4ato.3.6 has lobula phenotype, non-enhanceable by atoUAS.cJa, Scer\GAL4ato.3.6
Nintra.GS.UAS, Scer\GAL4ato.3.6 has medulla phenotype, non-enhanceable by atoUAS.cJa, Scer\GAL4ato.3.6
Nintra.GS.UAS, Scer\GAL4wor.PA has type II neuroblast | increased number | larval stage phenotype, suppressible by kluunspecified/kluunspecified
Nintra.GS.UAS, Scer\GAL4Act5C.PP has type I neuroblast | increased number | somatic clone | larval stage phenotype, suppressible by kluunspecified/kluunspecified
Nintra.GS.UAS, Scer\GAL4GMR9D11 has larval neuroblast | ectopic phenotype, suppressible by ermUAS.Tag:HA, Scer\GAL4GMR9D11
Nintra.GS.UAS, Scer\GAL4eg-Mz360 has larval ventral nerve cord phenotype, non-suppressible by hbUAS.cWa, Scer\GAL4eg-Mz360
Nintra.GS.UAS, Scer\GAL4109-30 has stalk follicle cell phenotype, non-suppressible by Six4HM05254, Scer\GAL4109-30
Nintra.GS.UAS, Scer\GAL4109-30 has basal stalk phenotype, non-suppressible by Six4HM05254, Scer\GAL4109-30
Nintra.GS.UAS, Scer\GAL4Act5C.PP has eye disc | somatic clone phenotype, non-suppressible by Vha55j2E9
Nintra.GS.UAS, Scer\GAL4pros.PMG has larval EL neuron phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has RP2sib neuron | increased number phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has U neuron | increased number phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has Usib neuron phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has dMP2 neuron phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has vMP2 neuron | increased number phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4pros.PMG has larval RP2 motor neuron phenotype, non-suppressible by spdoG104/spdoG104
Nintra.GS.UAS, Scer\GAL4ato.3.6 has lobula phenotype, non-suppressible by atoUAS.cJa, Scer\GAL4ato.3.6
Nintra.GS.UAS, Scer\GAL4ato.3.6 has medulla phenotype, non-suppressible by atoUAS.cJa, Scer\GAL4ato.3.6
Nintra.GS.UAS, Scer\GAL4Bx-MS1096 is an enhancer of wing disc | larval stage phenotype of NkapGD11807, Scer\GAL4Bx-MS1096
Scer\GAL4Act.PU/Nintra.GS.UAS is an enhancer of polar follicle cell | increased number | somatic clone phenotype of AxnS044230
Scer\GAL4Act.PU/Nintra.GS.UAS is an enhancer of stalk follicle cell | increased number | somatic clone phenotype of AxnS044230
Scer\GAL4Act.PU/Nintra.GS.UAS is an enhancer of basal stalk | somatic clone phenotype of AxnS044230
Nintra.GS.UAS, Scer\GAL4dpp.blk1 is a suppressor of wing disc phenotype of Pvrλ.UASp.Tag:MYC, Scer\GAL4dpp.blk1
Scer\GAL4GMR45D11/Nintra.GS.UAS is a suppressor | partially of posterior terminal follicle cell | oogenesis stage S7 phenotype of RpS5bCRISPR
Scer\GAL4GMR45D11/Nintra.GS.UAS is a suppressor | partially of posterior terminal follicle cell | oogenesis stage S8 phenotype of RpS5bCRISPR
Scer\GAL4Act5C.PP/Nintra.GS.UAS is a suppressor of eye disc | somatic clone phenotype of Vha55j2E9
Nintra.GS.UAS is a suppressor of macrochaeta & wing | ectopic phenotype of Mmus\Neurog1UAS.cQa, Scer\GAL4dpp.blk1
Scer\GAL4VP16.F442A.αTub84B/Nintra.GS.UAS is a suppressor of embryonic neuroblast | increased number phenotype of PsnC2
Scer\GAL4btl.PS/Nintra.GS.UAS is a non-suppressor of embryonic/larval ganglionic tracheal branch phenotype of seqZ1241
Scer\GAL4Tub.PU/Nintra.GS.UAS is a non-suppressor of follicle stem cell | somatic clone phenotype of mam8
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has larval brain | larval stage phenotype
Df(2L)erm2, Nintra.GS.UAS, Scer\GAL4erm.PU has type II neuroblast | increased number | larval stage phenotype
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has larval brain | larval stage phenotype
Df(2L)Exel7071, Nintra.GS.UAS, Scer\GAL4erm.PU has type II neuroblast | increased number | larval stage phenotype
Nintra.GS.UAS, PsnC2, Scer\GAL4VP16.F442A.αTub84B has wing | ectopic phenotype
The additional clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU enhances the supernumerary polar/stalk cell phenotype induced by AxnS044230 homozygous follicle cell clones.
The co-expression of Six4HM05254, does not suppress the increased number of basal stalk cells and the basal stalk defects induced by the expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood.
Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts and restores intermediate neural progenitor cells and ganglion mother cells in type II neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4wor.PA (analysed 72 hours after clone induction).
Type I neuroblast clones in the larval brain co-expressing kluScer\UAS.T:Ivir\HA1 and Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP contain an average of 18 neuroblasts per clone and 100% of the clones show the supernumerary neuroblast phenotype.
Homozygosity for kluunspecified significantly reduces the number of supernumerary neuroblasts per clone as well as the frequency of the clones containing more than one neuroblast in type I neuroblast clones in the larval brain expressing Nintra.GS.Scer\UAS under the control of Scer\GAL4Act5C.PP.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4btl.PS in seqZ1241 mutant trachea is not sufficient to suppress the ganglionic branch bifurcation phenotype. Activation of N in tracheal cells does not inhibit migration of ganglionic branch cells and is not able to overcome the effects of higher FGF in the ganglionic branches surrounding tissues of seqZ1241 mutants.
A Vha55j2E9 mutant clone background does not suppress the overproliferation phenotype seen when Nintra.GS.Scer\UAS is expressed in eye disc clones under the control of Scer\GAL4Act5C.PP.
Coexpression of ermScer\UAS.T:Ivir\HA under the control of Scer\GAL4CG31670.R9D11 suppresses the ectopic neuroblasts induced by expression of Nintra.GS.Scer\UAS.
Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub has no effect on the maintenance of mam8 follicle stem cell clones.
The ability of ptcS2 to induce follicle stem cell duplications in clones and for the clones to take over the whole ovariole is suppressed by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub. The loss of follicle stem cell clones caused by expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4tub is not substantially suppressed by ptcS2.
Nintra.GS.Scer\UAS; Scer\GAL4αTub84B.PL; wgl-17/wgl-17 somatic clones in the wing disc overproliferate, and non-autonomously induce overproliferation. However, unlike when such clones are wg+, this phenotype is mainly restricted to clones in the hinge and pleura, and is rarely seen in clones located in proximal regions of the wing pouch. Flow cytometry analysis of cell cycle progression in the mutant cells shows no difference in profile compared to wild-type cells.
The transformation of neurons (RP2 to RP2sib; dMP2 to vMP2; Usib to U) seen in Nintra.GS.Scer\UAS; Scer\GAL4pros.PMG embryos is unaffected by spdoG104/spdoG104.
PsnC2 clones that also express Nintra.GS.Scer\UAS lead to double dorsal wing outgrowth.
G. Struhl