FlyBase Allele Report: Dmel\mir-ban[EP3622]

General Information

Symbol

Dmel\mir-ban^EP3622

Species

D. melanogaster

Name

FlyBase ID

FBal0144429

Feature type

allele

Associated gene

Dmel\mir-ban

Associated Insertion(s)

P{EP}EP3622a

P{EP}EP3622b

Carried in Construct

Also Known As

ban^EP3622, EP(3)3622

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hipfner et al., 2002)

Mutagen

P-element activity

(Raisin et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{EP}EP3622a

P{EP}EP3622b

Cytology

Description

The "EP(3)3622" line contains two P{EP} elements (P{EP}ban^EP3622a and P{EP}ban^EP3622b) inserted in a back-to-back orientation at 61C in contig AE003469.

(Hipfner et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Kadener et al., 2009)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Kadener et al., 2009)

decreased body size

(Raisin et al., 2003, Hipfner et al., 2002)

decreased body size (with Df(3L)emc)

(Raisin et al., 2003)

decreased body size (with Df(3L)mir-ban^Δ1)

(Raisin et al., 2003, Hipfner et al., 2002)

decreased body size (with mir-ban^UY3207)

(Raisin et al., 2003)

decreased cell death, with Scer\GAL4^GMR.PF

(Thompson and Cohen, 2006)

decreased cell number

(Raisin et al., 2003)

decreased cell number (with Df(3L)mir-ban^Δ1)

(Raisin et al., 2003, Hipfner et al., 2002)

decreased cell number (with mir-ban^UY3207)

(Raisin et al., 2003)

decreased cell number | adult stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

decreased cell number | adult stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

decreased cell number | cell non-autonomous, with Scer\GAL4^en-e16E

(Resino and Garcia-Bellido, 2004)

decreased cell number | larval stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

decreased cell number | larval stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

decreased cell number | pupal stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

decreased cell number | pupal stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

decreased cell size, with Scer\GAL4^ap-md544

(Resino and Garcia-Bellido, 2004)

decreased cell size, with Scer\GAL4^en-e16E

(Resino and Garcia-Bellido, 2004)

female semi-sterile

(Hipfner et al., 2002)

female semi-sterile (with Df(3L)mir-ban^Δ1)

(Hipfner et al., 2002)

increased cell death

(Jaklevic et al., 2008)

increased cell death (with mir-ban^L1170a)

(Jaklevic et al., 2008)

increased cell number | cell autonomous, with Scer\GAL4^en-e16E

(Resino and Garcia-Bellido, 2004)

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Hipfner et al., 2002)

viable

(Hipfner et al., 2002)

viable (with Df(3L)mir-ban^Δ1)

(Hipfner et al., 2002)

visible, with Scer\GAL4^ap-md544

(Resino and Garcia-Bellido, 2004)

visible, with Scer\GAL4^Bx-MS1096

(Hipfner et al., 2002)

visible, with Scer\GAL4^en-e16E

(Resino and Garcia-Bellido, 2004)

visible, with Scer\GAL4^GMR.PF

(Hipfner et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Thompson and Cohen, 2006, Hipfner et al., 2002)

female germline stem cell | adult stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

female germline stem cell | adult stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

female germline stem cell | larval stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

female germline stem cell | larval stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

female germline stem cell | pupal stage | somatic clone (with Df(3L)mir-ban^Δ1)

(Shcherbata et al., 2007)

female germline stem cell | pupal stage | somatic clone (with mir-ban^L1170a)

(Shcherbata et al., 2007)

interommatidial bristle | increased number, with Scer\GAL4^GMR.PF

(Nolo et al., 2006, Thompson and Cohen, 2006)

mesonotum, with Scer\GAL4^ap-md544

(Resino and Garcia-Bellido, 2004)

microchaeta, with Scer\GAL4^ap-md544

(Resino and Garcia-Bellido, 2004)

pigment cell | increased number, with Scer\GAL4^GMR.PF

(Nolo et al., 2006)

wing

(Raisin et al., 2003)

wing, with Scer\GAL4^Bx-MS1096

(Hipfner et al., 2002)

wing, with Scer\GAL4^en-e16E

(Hipfner et al., 2002)

wing (with Df(3L)emc)

(Raisin et al., 2003)

wing (with Df(3L)mir-ban^Δ1)

(Raisin et al., 2003)

wing (with mir-ban^UY3207)

(Raisin et al., 2003)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Resino and Garcia-Bellido, 2004)

Detailed Description

Statement

Reference

Expression of ban^EP3622 under the control of Scer\GAL4^ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.

(Panneton et al., 2015)

Expression of ban^EP3622 under the control of Scer\GAL4^tim.PE results in a dramatically lengthened circadian period when locomotor activity is assayed (the increase is almost 3 hours).

Expression of ban^EP3622 under the control of Scer\GAL4^P2.4.Pdf results in a lengthened circadian period when locomotor activity is assayed.

(Kadener et al., 2009)

ban^EP3622 eye disc clones show no cell death.

(Tanaka-Matakatsu et al., 2009)

ban^EP3622 homozygotes or ban^EP3622/Df(3L)ban^Δ1 hemizygotes survive to adulthood and show wild-type levels of apoptosis. Imaginal discs in ban^EP3622 homo- and hemizygotes or ban^EP3622/ban^L1170a transheterozygotes show significantly higher levels of AO staining than wild-type, indicating that these mutants undergo IR-induced apoptosis more readily than wild-type. Phenotypes for ban^EP3622 homozygotes are more severe than those of ban^EP3622/Df(3L)ban^Δ1 hemizygotes.

ban^EP3622 homozygotes, ban^EP3622/ban^L1170a transheterozygotes and ban^EP3622/Df(3L)ban^Δ1 hemizygotes exposed to 0-8000 R of X-rays show decreased survival to adulthood after irradiation.

(Jaklevic et al., 2008)

ban^L1170a/ban^EP3622 mutant germline stem cells (GSCs) show maintenance and cell division defects when clones are generated during adult stages. When ban^L1170a/ban^EP3622 clones are generated in larval and pupal stages the GSC loss is less severe.

ban^EP3622/Df(3L)ban^Δ1 mutant germline stem cells (GSCs) show maintenance and cell division defects when clones are generated during adult stages. When ban^EP3622/Df(3L)ban^Δ1 clones are generated in larval and pupal stages the GSC loss is less severe.

(Shcherbata et al., 2007)

Expression of ban^EP3622 in the developing eye, under the control of Scer\GAL4^GMR.PF, causes the generation of extra interommatidial pigment and bristle cells.

(Nolo et al., 2006)

Expression of ban^EP3622 in the eye, driven by Scer\GAL4^GMR.PF, leads to eye overgrowth, due mainly to excess interommatidial cells. The number of cells undergoing apoptosis is reduced in these eyes, but this reduction is not great enough to account for all of the extra cells. The morphology of the eye is not affected.

(Thompson and Cohen, 2006)

Cells in the posterior compartment of the wing are 30.1% smaller than in controls in flies expressing ban^EP3622 under the control of Scer\GAL4^en-e16E. The area of the posterior compartment is increased by 19.1% in these flies compared to controls, and the number of cells in the posterior compartment is increased by 68.7%. The number of cells in the anterior compartment in flies expressing ban^EP3622 under the control of Scer\GAL4^en-e16E is reduced by 12.7% compared to controls. Expression of ban^EP3622 under the control of Scer\GAL4^ap-md544 results in a large increase in notum size and reduction in cell size in the notum. The density of chaetae in the acrostical region is increased by 17.5% compared to controls and is associated with a lower number of cells between microchaetae (4.52 compared to 5.34 in wild type). The total number of chaetae per heminotum is increased by 49% compared to wild type.

(Resino and Garcia-Bellido, 2004)

The body mass of ban^EP3622/Df(3L)emc flies is 68% that of control flies. The wing blade surface area is 84.3% that of controls. The body mass of ban^EP3622/Df(3L)ban^Δ1 flies is 80% that of control flies. The wing blade surface area is 89.6% that of controls. Cell size in the wing blade is 99.2% of controls and cell number in the wing blade is 91.1% that of controls. Heterozygotes have a normal body mass compared to controls. The body mass of homozygotes is 82% that of control flies. The wing blade surface area is 91.2% that of controls. Cell size in the wing blade is 100% of controls and cell number in the wing blade is 91.1% that of controls. The body mass of ban^EP3622/ban^UY3207 flies is 84% that of control flies. The wing blade surface area is 92.6% that of controls. Cell size in the wing blade is 99.3% of controls and cell number in the wing blade is 92.8% that of controls.

(Raisin et al., 2003)

The body mass of ban^EP3622/Df(3L)ban^Δ1 adults is only 78% of that of control flies. The body mass of homozygous adults is only 80% of that of control flies. The wing blade area of ban^EP3622/Df(3L)ban^Δ1 flies is only 90% of that of controls. This decrease is entirely due to a decrease in the number of cells, and is not due to a decrease in cell size. Expression of ban^EP3622 under the control of Scer\GAL4^en-e16E results in a significant increase in the ratio of posterior:anterior area of the wing compared to control wings. There is a statistically significant increase in the overall size of the wing compared to controls. Expression of ban^EP3622 under the control of Scer\GAL4^GMR.PF results in roughening and bulging of the eye, suggesting extensive outgrowth. Clones of cells expressing ban^EP3622 under the control of Scer\GAL4^Act5C.PP show the same distribution of cells in G1, S and G2 phases compared to wild-type cells, but the cells are 3% larger than the control cells. The cell doubling time is significantly reduced compared to controls. Expression of ban^EP3622 under the control of Scer\GAL4^Bx-MS1096 results in significant outgrowth of the entire wing, with the wings curving downwards.

(Hipfner et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mir-ban^EP3622 has radiation sensitive phenotype, enhanceable by Df(3L)Exel6115/+

(Bilak et al., 2014)

mir-ban^EP3622 has radiation sensitive phenotype, enhanceable by Df(3L)Exel9028/+

(Bilak et al., 2014)

Suppressed by

Statement

Reference

mir-ban^EP3622 has radiation sensitive phenotype, suppressible by Df(3L)Exel6086/+

(Bilak et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mir-ban^EP3622 has visible phenotype, non-suppressible by Cdk4³/Cdk4³

(Hipfner et al., 2002)

Enhancer of

Statement

Reference

mir-ban^EP3622 is an enhancer of increased cell death | somatic clone phenotype of Rbf^15aΔ

(Tanaka-Matakatsu et al., 2009)

mir-ban^EP3622/Df(3L)mir-ban^Δ1 is an enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.GMR.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3622 is an enhancer of increased occurrence of cell division | larval stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^ptc-559.1

(Brennecke et al., 2003)

mir-ban^EP3622 is an enhancer of decreased cell death | larval stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^ptc-559.1

(Brennecke et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/mir-ban^EP3622 is a suppressor | partially of abnormal cell size | somatic clone phenotype of yki^B5

(Nolo et al., 2006)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, hpo^UAS.cUa

(Nolo et al., 2006)

mir-ban^EP3622/Scer\GAL4^Tub.PU is a suppressor of cell lethal | somatic clone phenotype of yki^Δ.w

(Thompson and Cohen, 2006)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of hid^Ala5.GMR

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of hid^GMR.PG

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of rpr^GMR.PH

(Brennecke et al., 2003)

Scer\GAL4^ptc-559.1/mir-ban^EP3622 is a suppressor of increased cell death phenotype of Scer\GAL4^ptc-559.1, hid^EP30060

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Other

Statement

Reference

Scer\GAL4^Tub.PU, mir-ban^EP3622, yki^Δ.w has increased cell size | somatic clone phenotype

(Thompson and Cohen, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, mir-ban^EP3622 has interommatidial bristle | increased number phenotype, enhanceable by CycE^UAS.cRa, Scer\GAL4^GMR.PF

(Nolo et al., 2006)

Scer\GAL4^GMR.PF, mir-ban^EP3622 has pigment cell | increased number phenotype, enhanceable by CycE^UAS.cRa, Scer\GAL4^GMR.PF

(Nolo et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mir-ban^EP3622 has wing phenotype, non-suppressible by Cdk4³/Cdk4³

(Hipfner et al., 2002)

Enhancer of

Statement

Reference

mir-ban^EP3622 is an enhancer of eye disc | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Tanaka-Matakatsu et al., 2009)

mir-ban^EP3622/Df(3L)mir-ban^Δ1 is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.GMR.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3622/Df(3L)mir-ban^Δ1 is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.GMR.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3622, Scer\GAL4^ptc-559.1 is an enhancer of wing disc phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^ptc-559.1

(Brennecke et al., 2003)

Suppressor of

Statement

Reference

mir-ban^EP3622/Scer\GAL4^Tub.PU is a suppressor of wing disc | somatic clone | cell autonomous phenotype of yki^B5

(Neto-Silva et al., 2010)

Scer\GAL4^nub-AC-62/mir-ban^EP3622 is a suppressor of wing phenotype of hpo^UAS.cUa

(Nolo et al., 2006)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, hpo^UAS.cUa

(Nolo et al., 2006)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, hpo^UAS.cUa

(Nolo et al., 2006)

Scer\GAL4^hh-Gal4/mir-ban^EP3622 is a suppressor | partially of wing disc | somatic clone phenotype of yki^B5

(Nolo et al., 2006)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hid^Ala5.GMR

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hid^GMR.PG

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of rpr^GMR.PH

(Brennecke et al., 2003)

mir-ban^EP3622/Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

mir-ban^EP3622/Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

Other

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^Tub.PU, mir-ban^EP3622, yki^B5 has wing disc | somatic clone | cell autonomous phenotype

(Neto-Silva et al., 2010)

CycE^UAS.cRa, Diap1^UAS.cHa, Scer\GAL4^GMR.PF, mir-ban^EP3622 has interommatidial bristle | increased number phenotype

(Nolo et al., 2006)

CycE^UAS.cRa, Diap1^UAS.cHa, Scer\GAL4^GMR.PF, mir-ban^EP3622 has cone cell | increased number phenotype

(Nolo et al., 2006)

CycE^UAS.cRa, Diap1^UAS.cHa, Scer\GAL4^GMR.PF, mir-ban^EP3622 has eye photoreceptor cell | increased number phenotype

(Nolo et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Df(3L)Exel6115 suppresses the increased sensitivity to irradiation seen in ban^EP3622 mutants.

One copy of Df(3L)Exel6086 suppresses the increased sensitivity to irradiation seen in ban^EP3622 mutants.

(Bilak et al., 2014)

Expression of ban^EP3622 in wing disc clones (under the control of Scer\GAL4^tub.PU) in yki^B5 mutant animals increases clone size compared to yki^B5 alone. Co-expression of dm^Scer\UAS.cZa in this background fails to rescue the growth defect of yki^B5 mutant clones, and growth rates are similar to when ban^EP3622 is expressed alone in yki^B5 mutant discs. However, despite the small size of the clones, cell and nucleolar size are increased when dm^Scer\UAS.cZa and ban^EP3622 are expressed in yki^B5 wing discs.

(Neto-Silva et al., 2010)

A significant increase in the level of cell death is observed in Rbf^15aΔ;ban^EP3622 double mutant eye disc clones.

(Tanaka-Matakatsu et al., 2009)

Coexpression of ban^EP3622 with hpo^Scer\UAS.cUa, both driven by Scer\GAL4^GMR.PF, significantly suppresses the eye defects and eliminates the apoptosis caused by hpo overexpression.

Coexpression of ban^EP3622 with hpo^Scer\UAS.cUa in the wing, driven by Scer\GAL4^nub-AC-62, suppresses the Scer\GAL4^nub-AC-62>hpo^Scer\UAS.cUa small wing phenotype.

Coexpression of CycE^Scer\UAS.cRa with ban^EP3622, under the control of Scer\GAL4^GMR.PF, enhances the extra ommatidial cell phenotype seen in Scer\GAL4^GMR.PF>ban^EP3622 pupal retinae. This phenotype is not enhanced by th^Scer\UAS.cHa.

Coexpression of ban^EP3622, CycE^Scer\UAS.cRa and th^Scer\UAS.cHa, under the control of Scer\GAL4^GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells.

(Nolo et al., 2006)

Expression of ban^EP3622, under the control of Scer\GAL4^tub, in yki^Δ.w mutant clones generated in the wing disc rescues the lethality of these clones, resulting in clones that are larger than those generated in wild-type controls.

(Thompson and Cohen, 2006)

When E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa are driven by Scer\GAL4^ptc-559.1 cells in the wing disc overproliferate. The addition of ban^EP3622 enhances this phenotype. Fewer cells show pyknotic nuclei, though many cells still drop out of the epithelial layer.

(Brennecke et al., 2003)

EP3219^EP3219 combined with ban^EP3622 has little or no effect on growth. The wing overgrowth phenotype caused by expression of ban^EP3622 under the control of Scer\GAL4^Bx-MS1096 is not altered by Cdk4³/Cdk4³. Co-expression of ban^EP3622 almost completely suppresses the eye phenotype caused by expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev.

(Hipfner et al., 2002)

Xenogenetic Interactions

Statement

Reference

Reduction of ban activity through ban^EP3622/Df(3L)ban^Δ1 enhances Hsap\MJD^{tr.Q78.GMR.T:Ivir\HA1}-induced degeneration, such that flies are now born with more neuronal loss.

(Bilen et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

^EP3622

(Tanaka-Matakatsu et al., 2009)

EP(3)3622

(Resino and Garcia-Bellido, 2004, Brennecke et al., 2003)

EP3622

(Raisin et al., 2003)

EP³3622

(Kadener et al., 2009)

ban^EP3622

(Panneton et al., 2015, Bilak et al., 2014, Yang et al., 2009, Jaklevic et al., 2008, Shcherbata et al., 2007, Meyer et al., 2006)

ban^EP

(Gerlach et al., 2019)

banEP

(Neto-Silva et al., 2010)

mir-ban^EP3622

Name Synonyms

Secondary FlyBase IDs

References (20)

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