FlyBase Allele Report: Dmel\Pi3K92E[A2860C.UAS]

General Information

Symbol

Dmel\Pi3K92E^A2860C.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156079

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K92E.A2860C}

Also Known As

UAS-PI3K^DN, PI3K^DN, UAS-PI3K92E.A2860C, UAS-PI3K-DN

Key Links

Transgenic product class

dominant_negative_variant

(Parks, 2004.5.12)

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Parks, 2004.5.12)

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{UAS-Pi3K92E.A2860C}

Cytology

Description

Nucleotide substitution: A2860C. Amino acid replacement: D?A. Pi3K92E sequences were cloned into P{Express-UAS}. The A2860C mutation changes an Asp to Ala in the ATP binding site and results in a dominant negative allele. Expression is governed by UAS regulatory sequences.

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K92E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage, with Scer\GAL4^elav-C155

(Saedi et al., 2024)

chemical resistant, with Scer\GAL4^elav-C155

(Eddison et al., 2011)

chemical resistant | adult stage | heat sensitive, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

(Eddison et al., 2011)

decreased cell size | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

decreased cell size | larval stage, with Scer\GAL4^sns.PU

(Gass et al., 2022)

decreased cell size | larval stage | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Cinnamon et al., 2016)

decreased occurrence of cell division | larval stage, with Scer\GAL4^nab

(Sousa-Nunes et al., 2011)

decreased occurrence of cell division | second instar larval stage, with Scer\GAL4^repo.PU

(Sousa-Nunes et al., 2011)

visible, with Scer\GAL4^ap-md544

(Lee et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Cliffe et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Somatic clones in the larval fat body expressing Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} display a decrease in fat body cell size, but do not display significant difference in lipid droplet content, as compared to controls.

Larval oenocyte clones expressing Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} display significantly decreased size of oenocytes, as compared to controls.

Expression of Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^Desat1.PB in larvae results in decreased size of oenocytes in both fed and starvation conditions.

(Cinnamon et al., 2016)

Neuronal expression of Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^elav-C155 decreases sensitivity to ethanol sedation.

Neuronal expression of Pi3K92E^{A2860C.Scer\UAS} (under the control of Scer\GAL4^elav-C155) throughout development (until eclosion of the adult fly, when Scer\GAL80^ts.αTub84B is induced) results in reduced ethanol sensitivity. The converse experiment, with over-expression in neurons only after eclosion, does not alter ethanol sensitivity.

(Eddison et al., 2011)

Central nervous system neuroblasts of fed larvae expressing Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^nab show a reduction in EdU incorporation compared to controls.

Central nervous system neuroblasts of fed second instar larvae expressing Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^repo.PU show a decrease in EdU incorporation compared to controls.

(Sousa-Nunes et al., 2011)

Expression of Pi3K92E^{A2860C.Scer\UAS} driven by Scer\GAL4^ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.

(Lee et al., 2010)

Expression of Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 results in an up-curled wing phenotype in 27.5% of flies and a down-curled wing phenotype in 10.5% of flies.

(Cliffe et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

NOT Enhanced by

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^elav-C155 has abnormal starvation stress response | adult stage phenotype, non-enhanceable by mir-137^CR

(Saedi et al., 2024)

Pi3K92E^A2860C.UAS, Scer\GAL4^elav-C155 has abnormal starvation stress response | adult stage phenotype, non-enhanceable by mir-137^KO

(Saedi et al., 2024)

Suppressed by

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB has decreased cell size | larval stage phenotype, suppressible by FarO^GD3202, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Pi3K92E^A2860C.UAS, Scer\GAL4^elav-C155 has chemical resistant phenotype, suppressible by aru^8.128

(Eddison et al., 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/Pi3K92E^A2860C.UAS is a non-enhancer of abnormal starvation stress response | adult stage | semidominant phenotype of mir-137^CR

(Saedi et al., 2024)

Scer\GAL4^elav-C155/Pi3K92E^A2860C.UAS is a non-enhancer of abnormal starvation stress response | adult stage | semidominant phenotype of mir-137^KO

(Saedi et al., 2024)

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB is a non-enhancer of abnormal starvation stress response | larval stage phenotype of Scer\GAL4^Desat1.PB, spidey^NIG.1444R

(Cinnamon et al., 2016)

Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^{Act5C.Switch.PR} is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087

(Chen et al., 2022)

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB is a suppressor | partially of increased cell size | larval stage phenotype of Scer\GAL4^Desat1.PB, spidey^NIG.1444R

(Cinnamon et al., 2016)

NOT Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB is a non-suppressor of abnormal starvation stress response | larval stage phenotype of Scer\GAL4^Desat1.PB, spidey^NIG.1444R

(Cinnamon et al., 2016)

Scer\GAL4^elav-C155/Pi3K92E^A2860C.UAS is a non-suppressor of chemical sensitive phenotype of aru^8.128

(Eddison et al., 2011)

Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a non-suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Other

Statement

Reference

Ent1^GD783, Pi3K92E^A2860C.UAS, Scer\GAL4^Bx-MS1096 has visible phenotype

(Cliffe et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^ap-md544 has wing blade phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

Suppressed by

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB has embryonic/larval oenocyte | larval stage phenotype, suppressible by FarO^GD3202, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

NOT Enhancer of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB is a non-enhancer of embryonic/larval oenocyte | larval stage phenotype of Scer\GAL4^Desat1.PB, spidey^NIG.1444R

(Cinnamon et al., 2016)

Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB is a suppressor | partially of embryonic/larval oenocyte | larval stage phenotype of Scer\GAL4^Desat1.PB, spidey^NIG.1444R

(Cinnamon et al., 2016)

NOT Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a non-suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Other

Statement

Reference

Ent1^GD783, Pi3K92E^A2860C.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype

(Cliffe et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of FarO^GD3202 suppresses the increased lipid droplet content of oenocytes seen in fed larvae, and suppresses the decreased oenocyte size of both fed and starved larvae expressing Pi3K92E^{A2860C.Scer\UAS} under the control of Scer\GAL4^Desat1.PB.

Co-expression of Pi3K92E^{A2860C.Scer\UAS} does not enhance or suppress the increase in lipid droplets in larval oenocytes seen in starved larvae, but it partially suppresses the increased oenocyte size in both fed and starved larvae expressing spidey^NIG.1444R under the control of Scer\GAL4^Desat1.PB.

(Cinnamon et al., 2016)

Flies over-expressing Pi3K92E^{A2860C.Scer\UAS} in neurons (with Scer\GAL4^elav-C155) in an aru^8.128 background show increased ethanol sensitivity, indicating suppression of the Pi3K92E^{A2860C.Scer\UAS} phenotype.

(Eddison et al., 2011)

Co-expression of Sesn^d04539 with Pi3K92E^{A2860C.Scer\UAS} driven by Scer\GAL4^ap-md544 enhances the wing-bending phenotype of Pi3K92E^{A2860C.Scer\UAS}.

(Lee et al., 2010)

100% of flies co-expressing Pi3K92E^{A2860C.Scer\UAS} and Ent1^GD783 under the control of Scer\GAL4^Bx-MS1096 have a strongly up-curled phenotype.

(Cliffe et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8288

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.A2860C}1

8289

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.A2860C}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pi3K92E^{A2860C.Scer\UAS}

Pi3K92E^A2860C.UAS

p110^DN

(Sousa-Nunes et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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