FlyBase Allele Report: Dmel\fru[P1.LexA]

General Information

Symbol

Dmel\fru^P1.LexA

Species

D. melanogaster

Name

FlyBase ID

FBal0245975

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

TI{lexA::VP16}fru^P1.LexA

Carried in Construct

Also Known As

fru-LexA, fru^LexA, FruP1-LexA, fru^P1-LexA, FruLexA

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Mellert et al., 2010)

I-SceI

(Mellert et al., 2010)

FLPase

(Mellert et al., 2010)

ends-out gene targeting

(Mellert et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{lexA::VP16}fru^P1.LexA

Cytology

Description

Sequence encoding a lexA::VP16 driver has been inserted at the first codon of the fru[M] open reading frame. This allows for expression of the lexA::VP16 driver in the same pattern as transcripts from the fru[M] (P1) promoter, which is transcribed in both males and females.

(Mellert et al., 2010)

Allele components

Component

Use(s)

Inserted element

TI{lexA::VP16}

promoter trap

Encoded product / tool

lexA::VP16

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior (with fru^4-40)

(Chen et al., 2021)

abnormal neuroanatomy | adult stage | female (with fru^sat15), with Scer\GAL4^Poxn.14, fru^{MC.UAS.Tag:AU1}

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male (with fru^4-40)

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male (with fru^sat15)

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male (with fru^ΔC)

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male limited (with fru^4-40)

(Zhou et al., 2015)

abnormal neuroanatomy | male limited (with fru^FLP)

(Tran et al., 2014)

decreased cell number | adult stage | male limited (with fru^4-40)

(Zhou et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

fru^4-40/fru^P1.LexA adult males do not have the male-specific ventrolateral protocerebrum projection neuron 1; there is no effect on its absence from the female.

(Zhou et al., 2015)

mSG neuron projections in the subesophageal ganglion (SOG) are defective in the brains of fru^FLP/fru^P1.LexA adult males; the bilateral projections that usually extend toward the tritocerebrum are significantly reduced, similar to those seen in fru^FLP/+ females.

(Tran et al., 2014)

fru^P1.LexA has the expected characteristics of a "fru[M]" null allele.

Midline crossing by foreleg gustatory receptor neuron (GRN) axons is nearly absent in fru^P1.LexA/fru^P1.LexA, fru^P1.LexA/fru^4-40 and fru^P1.LexA/fru^sat15 XY animals.

Nearly all XY fru^P1.LexA/fru^ΔC animals show complete loss of midline crossing by foreleg GRN axons.

(Mellert et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

dsx^Δ, fru^P1.LexA, dsx¹, Df(3R)Exel6179 is a non-suppressor of increased occurrence of cell division | female limited | adult stage phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

Other

Statement

Reference

Dper\dsx^+t31M5, Dper\fru^+t36N18, dsx^Δ/dsx¹, fru^P1.LexA/fru^AJ96u3 has abnormal song phenotype

(Cande et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

dsx^Δ, fru^P1.LexA, dsx¹, Df(3R)Exel6179 is a non-suppressor of adult posterior midgut epithelium | female limited phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

dsx^Δ/dsx¹, fru^P1.LexA/Df(3R)Exel6179 individuals do not show significant changes in mitotic index in the female and male adult posterior midgut, as compared to controls.

fru^P1.LexA/Df(3R)Exel6179, dsx¹/dsx^Δ double transheterozygosity does not suppress the increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of N^KK102890 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression).

(Hudry et al., 2016)

The normal order of behaviours is seen in the courtship behaviours elicited in intact solitary males expressing TrpA1^{Scer\UAS.(B).cKa} under the control of either Scer\GAL4^fru.P1.B, Scer\GAL4^fru.P1.D, Scer\GAL4^dsx-GAL4 or Scer\GAL4^dsx.Δ2 at 27 or 29[o]C, in that wing extension is always induced before abdomen bending. This ordering is lost if the males are also carrying fru^P1.LexA/fru^4-40.

Solitary males expressing TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^dsx.Δ2 in a fru^P1.LexA/fru^4-40 background at 27[o]C show approximately equal unilateral and bilateral wing extension.

(Pan et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fru^P1.LexA/fru^sat15 is rescued by fru^{MC.UAS.Tag:AU1}/Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Comments

Male-typical crossing of the midline by foreleg gustatory receptor neuron (GRN) axons is restored in XY fru^P1.LexA/fru^sat15 animals by expression of fru^{MC.Scer\UAS.T:BPV-1\AU1} under the control of Scer\GAL4^Poxn.14.

(Mellert et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66698

w^*; wg^Sp-1/CyO; TI{lexA::VP16}fru^P1.LexA/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

fru^LexA

(Chen et al., 2021, Sun et al., 2020, Wu et al., 2019, Machado et al., 2017, Huang et al., 2016, Hueston et al., 2016, Meissner et al., 2016, Zhou et al., 2015, Tran et al., 2014, Thistle et al., 2012, Pan et al., 2011)

fru^P-LexA

(Tran et al., 2014)

fru^P1-LexA

(Hudry et al., 2016)

fru^P1.LexA

(Wohl et al., 2020, Mellert et al., 2010)

fru^P1.lexA

(Feng et al., 2014)

fru^P1-LexA

(Thistle et al., 2012, Thistle et al., 2012)

fru^P1lexA

(Cande et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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