FlyBase Allele Report: Dmel\cnc[UAS.cSa]

General Information

Symbol

Dmel\cnc^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sykiotis

FlyBase ID

FBal0263741

Feature type

allele

Associated gene

Dmel\cnc

Associated Insertion(s)

Carried in Construct

P{UAS-cnc.C}

Also Known As

UAS-cncC, UAS cncC, UAS-Cnc

Key Links

Transgenic product class

wild_type

(Sykiotis and Bohmann, 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sykiotis and Bohmann, 2008)

Mutagen

in vitro construct

(Sykiotis and Bohmann, 2008)

Progenitor genotype

Carried in construct

P{UAS-cnc.C}

Cytology

Description

UASt regulatory sequences drive expression of the longest isoform (C) of wild-type cnc.

(Sykiotis and Bohmann, 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cnc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by l(2)gl^HMS01905

(Chatterjee et al., 2022)

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cTa

(Barone et al., 2011)

ameliorates intestinal cancer

modeled by N^RNAi.P.UAS

(Chen et al., 2021)

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}, Hsap\BACE1^UAS.Exel

(Tsakiri et al., 2021)

ameliorates Parkinson's disease 2

modeled by park^HMS01800

(Gumeni et al., 2021)

ameliorates Parkinson's disease 6

modeled by Pink1^HMC04160

(Gumeni et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

abnormal oxidative stress response, with Scer\GAL4^{αTub84B.Switch.PK}

(Sykiotis and Bohmann, 2008)

abnormal sleep | adult stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

short lived, with Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

visible, with Scer\GAL4^sev.EP

(Rahman et al., 2013, Sykiotis and Bohmann, 2008)

Phenotype Manifest In

embryonic/larval Malpighian tubule | larval stage, with Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

eye, with Scer\GAL4^sev.EP

(Rahman et al., 2013)

midgut | adult stage, with Scer\GAL4^NP5130

(Wang et al., 2014)

midgut | adult stage | somatic clone, with Scer\GAL4^Act.PU

(Wang et al., 2014)

ommatidium, with Scer\GAL4^sev.EP

(Sykiotis and Bohmann, 2008)

Detailed Description

Statement

Reference

Expression of cnc^UAS.cSa under the control of Scer\GAL4^elav-C155 leads to significant changes in neurophysiology of third instar larval neuromuscular junctions: potently increased spontaneous EJC (mEJC) amplitudes, decreased evoked EJC (eEJC) amplitudes, decreased quantal content (QC) and reduced number of releasable quanta (without any changes in paired-pulse ratios or mEJC frequencies) while it does not lead to any changes in third instar larval crawling distances or the life span of adults compared to controls.

Expression of cnc^UAS.cSa under the control of Scer\GAL4^elav-C155 leads to a significant decrease in sleep bout length and a significant increase in sleep bout number during day but not night time when compared to control adults. It does not lead to any changes in the total activity of adults as compared to controls.

(Spiers et al., 2019)

The expression for 6 days of cnc^UAS.cSa, either under the control of Scer\GAL4^NP5130 and temporally regulated by Gal80[ts], or in clones under the control of Scer\GAL4^Act.PU, leads to strong decreases in the proportion of mitotic cells in the adult midgut, as compared to controls.

(Wang et al., 2014)

Expression of cnc^Scer\UAS.cSa under the control of Scer\GAL4^sev.EP results in ommatidial loss and a rough eye phenotype.

(Rahman et al., 2013)

Expression of cnc^Scer\UAS.cSa under the control of Scer\GAL4^ple.PF has no effect on locomotor performance.

(Barone et al., 2011)

Overexpression of cnc^Scer\UAS.cSa in the eye under the control of Scer\GAL4^sev.EP disrupts the ommatidial arrangement, resulting in a rough eye appearance.

Overexpression of cnc^Scer\UAS.cSa under the control of Scer\GAL4^Switch.tub significantly increases the survival of both female and male flies after exposure to oxidative stress.

(Sykiotis and Bohmann, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sev.EP, cnc^UAS.cSa has visible phenotype, enhanceable by Scer\GAL4^sev.EP/maf-S^UAS.Tag:HA

(Rahman et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^sev.EP, cnc^UAS.cSa has visible phenotype, suppressible | partially by maf-S^RNAi.UAS/Scer\GAL4^sev.EP

(Rahman et al., 2013)

Scer\GAL4^sev.EP, cnc^UAS.cSa has visible phenotype, suppressible by Keap1^UAS.cSa, Scer\GAL4^sev.EP

(Sykiotis and Bohmann, 2008)

Suppressor of

Statement

Reference

cnc^UAS.cSa, Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | P-stage phenotype of Atg8a^HMS06041, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Scer\GAL4^NP5130/cnc^UAS.cSa is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, TER94^GL00448

(Liu et al., 2022)

Scer\GAL4^NP5130/cnc^UAS.cSa is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, TER94^HMS00656

(Liu et al., 2022)

cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of visible | adult stage | RU486 conditional phenotype of Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^elav.PLu

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^elav.PLu is a suppressor of decreased cell number | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^elav.PLu

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^elav.PLu is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^elav.PLu

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa is a suppressor of visible | adult stage | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800

(Gumeni et al., 2021)

cnc^UAS.cSa/Scer\GAL4^elav.PLu is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PLu, park^HMS01800

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Ero1L^GD3577, Scer\GAL4^NP5130

(Wang et al., 2015)

cnc^UAS.cSa, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^UAS.cTa, Scer\GAL4^ple.PF

(Barone et al., 2011)

cnc^UAS.cSa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^UAS.cTa, Scer\GAL4^ple.PF

(Barone et al., 2011)

NOT Suppressor of

Statement

Reference

cnc^UAS.cSa/Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^elav.PLu, park^HMS01800

(Gumeni et al., 2021)

cnc^UAS.cSa/Scer\GAL4^elav.PLu is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^elav.PLu, park^HMS01800

(Gumeni et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sev.EP, cnc^UAS.cSa has eye phenotype, enhanceable by Scer\GAL4^sev.EP/maf-S^UAS.Tag:HA

(Rahman et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^sev.EP, cnc^UAS.cSa has eye phenotype, suppressible | partially by maf-S^RNAi.UAS/Scer\GAL4^sev.EP

(Rahman et al., 2013)

Scer\GAL4^sev.EP, cnc^UAS.cSa has ommatidium phenotype, suppressible by Keap1^UAS.cSa, Scer\GAL4^sev.EP

(Sykiotis and Bohmann, 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, cnc^UAS.cSa has midgut | adult stage phenotype, non-suppressible by Xbp1^GD4745/Scer\GAL4^NP5130

(Wang et al., 2014)

Scer\GAL4^Act.PU, cnc^UAS.cSa has midgut | somatic clone | adult stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/Xbp1^GD4745

(Wang et al., 2014)

Enhancer of

Statement

Reference

cnc^UAS.cSa, Scer\GAL4^Mef2.PR is an enhancer of lysosome | adult stage | increased number phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

Suppressor of

Statement

Reference

cnc^UAS.cSa, Scer\GAL4^ppk.PU is a suppressor | partially of larval dorsal multidendritic neuron ddaC | P-stage phenotype of Atg8a^HMS06041, Scer\GAL4^ppk.PU

(Tan et al., 2024)

cnc^UAS.cSa, Scer\GAL4^ppk.PU is a suppressor | partially of dendrite | P-stage phenotype of Atg8a^HMS06041, Scer\GAL4^ppk.PU

(Tan et al., 2024)

Scer\GAL4^NP5130/cnc^UAS.cSa is a suppressor of adult midgut progenitor cell | increased number phenotype of Scer\GAL4^NP5130, TER94^GL00448

(Liu et al., 2022)

Scer\GAL4^NP5130/cnc^UAS.cSa is a suppressor of adult midgut progenitor cell | increased number phenotype of Scer\GAL4^NP5130, TER94^HMS00656

(Liu et al., 2022)

cnc^UAS.cSa, Scer\GAL4^Mef2.PR is a suppressor of adult somatic muscle cell phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^{αTub84B.Switch.PK} is a suppressor of wing | RU486 conditional phenotype of Pink1^HMC04160, Scer\GAL4^{αTub84B.Switch.PK}

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of mitochondrion | adult stage phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of adult somatic muscle cell phenotype of Pink1^HMC04160, Scer\GAL4^Mef2.PR

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^elav.PLu is a suppressor of dopaminergic PPL1 neuron | decreased number phenotype of Pink1^HMC04160, Scer\GAL4^elav.PLu

(Gumeni et al., 2021)

Scer\GAL4^Mef2.PR/cnc^UAS.cSa is a suppressor of adult somatic muscle cell phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

Scer\GAL4^Mef2.PR/cnc^UAS.cSa is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

Scer\GAL4^{αTub84B.Switch.PK}/cnc^UAS.cSa is a suppressor of wing | RU486 conditional phenotype of Scer\GAL4^{αTub84B.Switch.PK}, park^HMS01800

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

ref(2)P^HMS00551, Scer\GAL4^{αTub84B.Switch.PK}, cnc^UAS.cSa is a suppressor of adult somatic muscle cell phenotype of Scer\GAL4^Mef2.PR, park^HMS01800

(Gumeni et al., 2021)

cnc^UAS.cSa, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of Ero1L^GD3577, Scer\GAL4^NP5130

(Wang et al., 2015)

Scer\GAL4^NP5130/cnc^UAS.cSa is a suppressor of midgut | adult stage phenotype of Scer\GAL4^NP5130, Xbp1^GD4745

(Wang et al., 2014)

Scer\GAL4^Act.PU/cnc^UAS.cSa is a suppressor | somatic clone | partially of midgut | somatic clone | adult stage phenotype of Scer\GAL4^Act.PU, Xbp1^GD4745

(Wang et al., 2014)

NOT Suppressor of

Statement

Reference

cnc^UAS.cSa/Scer\GAL4^elav.PLu is a non-suppressor of dopaminergic PPL1 neuron | decreased number phenotype of Scer\GAL4^elav.PLu, park^HMS01800

(Gumeni et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell proliferation in the adult gut observed in flies expressing Ero1L^GD3577 under the control of Scer\GAL4^esg-NP5130 (and using tub-Gal80[ts] to limit the time of expression) is suppressed by co-expression of cnc^Scer\UAS.cSa.

(Wang et al., 2015)

Co-expression of maf-S^{Scer\UAS.T:Ivir\HA1} enhances the rough eye phenotype caused by expression of cnc^Scer\UAS.cSa under the control of Scer\GAL4^sev.EP.

Co-expression of maf-S^{dsRNA.Scer\UAS} suppresses the rough eye phenotype caused by expression of cnc^Scer\UAS.cSa under the control of Scer\GAL4^sev.EP.

(Rahman et al., 2013)

Co-expression of Keap1^Scer\UAS.cSa under the control of Scer\GAL4^sev.EP completely suppresses the cnc^Scer\UAS.cSa gain-of-function phenotype, restoring the eye to a wild-type morphology.

(Sykiotis and Bohmann, 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of cnc^Scer\UAS.cSa with Hsap\SNCA^Scer\UAS.cTa (specifically at 2-5 days after eclosion), under the control of Scer\GAL4^ple.PF, prevents the deficit in locomotor performance induced by Hsap\SNCA^Scer\UAS.cTa expression.

Expression of cnc^Scer\UAS.cSa at 25[o]C 2-5 days after eclosion has no effect on the age-associated decline in negative geotaxis found in flies expressing Hsap\SNCA^Scer\UAS.cTa under the control of Scer\GAL4^ple.PF.

(Barone et al., 2011)

Complementation and Rescue Data

Rescues

Scer\GAL4^ppk.PU/cnc^UAS.cSa rescues cnc¹²²³

(Chew et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

95246