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General Information
Symbol
Dmel\wdsRNA.GMR
Species
D. melanogaster
Name
FlyBase ID
FBal0265706
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GMR-wIR, GMR-wIR
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Consists of two copies of the w exon 3 in opposite orientation to each other and separated by a w intron. The construct is terminated with a Hsp70 terminator. The resulting transcript after intron splicing, is predicted to create a perfect hairpin dsRNA of length 629 bp.

The GMR promoter drives expression of a tail-to-tail w repeat. The repeat comprises the 629bp third exon of w ligated to the 74bp second intron of w, ligated to another copy of the w third exon in the opposite orientation to the first copy.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of wdsRNA.GMR/+ leads to pale orange eye color while expression of wdsRNA.GMR/wdsRNA.GMR leads to almost white eye color.

Two copies of the P{GMR-w.IR} transgene leads to an almost complete loss of eye pigmentation, while a single copy results in a less stringent eye phenotype, with a light yellow or orange eye color.

wdsRNA.GMR produces white eyes in a wild-type genetic background.

Flies carrying wdsRNA.GMR show a 90-fold reduction in drosopterin pigment levels compared to wild type.

Expression of wdsRNA.GMR suppresses eye pigmentation.

Flies carrying one copy of wdsRNA.GMR in a wild-type background have yellowish eyes.

Flies carrying wdsRNA.GMR have faint orange eyes.

Adult females transfected with one copy of wdsRNA.GMR have yellow or pale yellow eyes, depending on the insertion. All flies from each line exhibit a uniform eye colour phenotype, indicating strong penetrance and expressivity of the RNAi effect. Adults transfected with two copies of wdsRNA.GMR have white eyes, like flies that carry null w alleles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by E(Pc)[+]/E(Pc)w3

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Psc[+]/Psc1

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Psc[+]/Psce23

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Psce25/Psc[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Psch28/Psc[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2k06344/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)24d1/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2s15/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2[+]/Su(z)2s20

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2s95/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2sM/Su(z)2[+]

Suppressed by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has visible phenotype, suppressible by r2d21

NOT suppressed by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has visible phenotype, non-suppressible by loqs[+]/loqsKO

wdsRNA.GMR/wdsRNA.GMR has visible phenotype, non-suppressible by loqs[+]/loqsPA.Tag:FLAG/loqsKO

wdsRNA.GMR/wdsRNA.GMR has visible phenotype, non-suppressible by loqs[+]/loqsPB.Tag:FLAG/loqsKO

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by E(Pc)[+]/E(Pc)w3

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Psc[+]/Psc1

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Psc[+]/Psce23

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Psce25/Psc[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Psch28/Psc[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2k06344/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)24d1/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2s15/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2[+]/Su(z)2s20

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2s95/Su(z)2[+]

wdsRNA.GMR/wdsRNA.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2sM/Su(z)2[+]

Phenotype Manifest In
NOT Enhanced by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-enhanceable by Snpc00465/Snpc00465

Suppressed by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, suppressible by AGO2414

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, suppressible by Dcr-2G31R

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, suppressible by r2d21

NOT suppressed by
Statement
Reference

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-suppressible by loqs[+]/loqsKO

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-suppressible by loqs[+]/loqsPA.Tag:FLAG/loqsKO

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-suppressible by loqs[+]/loqsPB.Tag:FLAG/loqsKO

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-suppressible by piwi1/piwi1

wdsRNA.GMR/wdsRNA.GMR has eye phenotype, non-suppressible by aubHN2/aubN11

Additional Comments
Genetic Interactions
Statement
Reference

The eyes of wdsRNA.GMR transgenic female flies homozygous for any of AGO2414, Dcr-2L811fsX, Dcr-2G31R or r2d21 are similar to that of wild-type.

The reduction in drosopterin pigment levels seen in flies carrying wdsRNA.GMR is enhanced manyfold if they are also mutant for one of g1, HPS4W515X, p1 or ca1.

The reduction in drosopterin pigment levels seen in flies carrying wdsRNA.GMR is not enhanced by car1.

Flies carrying one copy of wdsRNA.GMR in a homozygous Dcr-2L811fsX background have red eyes.

The yellowish eye colour caused by expression of one copy of wdsRNA.GMR in a wild-type background is not altered if the flies are also homozygous for either piwi1 or piwi06843 or are carrying aubHN2/aubN11.

Dcr-2unspecified/Dcr-2unspecified substantially restores eye pigmentation in flies carrying wdsRNA.GMR.

Snpc00465/Snpc00465 does not enhance the loss of eye pigmentation seen in flies carrying wdsRNA.GMR.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Transfected into flies to study the phenotypic consequences of dsRNA interference (RNAi) of the w gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
wIR.GMR
wdsRNA.GMR
Name Synonyms
Secondary FlyBase IDs
  • FBal0191476
  • FBal0154368
References (28)