FlyBase Allele Report: Dmel\vtd[GL00522]

General Information

Symbol

Dmel\vtd^GL00522

Species

D. melanogaster

Name

FlyBase ID

FBal0266222

Feature type

allele

Associated gene

Dmel\vtd

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00522}

Also Known As

UAS-rad21^RNAi, vtd RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00522}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00522

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Cornelia de Lange syndrome 4

CEA

(MacPherson et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Eggs with expression of vtd^GL00522 driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} do not hatch. Embryos progress through syncytial divisions that become progressively aberrant and asynchronous, with suggestions of precocious chromosome segregation; eggs appear to complete meiosis, with presence of polar body chromosomes that lack centromeric cohesion in embryos.

Mature stage 14 oocytes with expression of vtd^GL00522 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} are indistinguishable from wild type; stage 9-11 oocytes are similar to wild type in terms of arm cohesion during meiosis.

(Guo et al., 2016)

Expression of vtd^GL00522 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not have any effect on the synaptonemal complex assembly and centromere clustering (assessed by number of cid foci) in oocytes.

(Gyuricza et al., 2016)

Expression of vtd^GL00522 (together with UAS-Dicer2 to enhance RNAi efficacy) under the control of Scer\GAL4^en-e16E to induce chromosome instability in the proliferative third instar larval wing disc tissue results in increased frequency of aneuploid metaphasic cells, a marked increase in autophagy (visualized by autophagy markers Atg8 and LysoTracker), oxidative stress (elevated levels of ROS) and cell death (visualized using [*]Casp3).

(Liu et al., 2016)

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) leads to increase in aneuploidy, increase in apoptosis, increased mitochondrial activity and increased oxidative stress in the posterior compartment of the third instar larval wing disc, as compared to controls.

The expression of vtd^GL00522 under the control of Scer\GAL4^Bx-MS1096 (together with Dicer-2, for efficient RNAi) induces low levels of apoptosis in the third instar larval wing pouch, as compared to controls.

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) increases the level of reactive oxygen species in the posterior compartment of the third instar larval wing disc, as compared to controls.

(Liu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, vtd^GL00522 has increased cell death | third instar larval stage phenotype, enhanceable by Scer\GAL4^en-e16E/park^KK107919

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has increased cell death | third instar larval stage phenotype, enhanceable by Atg1^GD7149, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has increased cell death | third instar larval stage phenotype, enhanceable by Atg18a^GD12342, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^Bx-MS1096, vtd^GL00522 has increased cell death | third instar larval stage phenotype, enhanceable by dl^UAS.GFP/Scer\GAL4^Bx-MS1096

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Liu et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by mTor^GL00156/Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^en-e16E/park^{UAS.Tag:FLAG,Tag:Mito(hCOX4I1)}

(Liu et al., 2016)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell number | third instar larval stage phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell number | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/egr^KK103432

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell number | third instar larval stage phenotype, suppressible by bsk^GD10555/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by dl^JF02825/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by Dsp1^JF02732, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/egr^KK103432

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell death | third instar larval stage phenotype, suppressible by bsk^GD10555/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell number | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has increased cell number | third instar larval stage phenotype, suppressible by dl^JF02825/Scer\GAL4^en.PU

(Liu et al., 2015)

NOT Enhancer of

Statement

Reference

vtd^GL00522, Scer\GAL4^{VP16.nanos.UTR} is a non-enhancer of abnormal cell cycle phenotype of SMC3^GL00518, Scer\GAL4^{VP16.nanos.UTR}

(Guo et al., 2016)

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, Sse^{RNAi.147.UASp}, vtd^GL00522 has abnormal cell cycle phenotype

(Guo et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, enhanceable by Scer\GAL4^en-e16E/park^KK107919

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has chromosome | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, enhanceable by Atg1^GD7149, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, enhanceable by Atg18a^GD12342, Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^Bx-MS1096, vtd^GL00522 has wing pouch | third instar larval stage phenotype, enhanceable by dl^UAS.GFP/Scer\GAL4^Bx-MS1096

(Liu et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by mTor^GL00156/Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^en-e16E/park^{UAS.Tag:FLAG,Tag:Mito(hCOX4I1)}

(Liu et al., 2016)

Scer\GAL4^en-e16E, vtd^GL00522 has chromosome | third instar larval stage phenotype, suppressible | partially by mTor^GL00156/Scer\GAL4^en-e16E

(Liu et al., 2016)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible | partially by dl^JF02825/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible | partially by Dsp1^JF02732, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^en.PU/egr^KK103432

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc posterior compartment | third instar larval stage phenotype, suppressible by bsk^GD10555/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by dl^JF02825/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by bsk^GD10555/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, suppressible by Rel^HM05154, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, suppressible by dl^JF02825/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, suppressible by bsk^GD10555/Scer\GAL4^en.PU

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, suppressible by Rel^HM05154, Scer\GAL4^en.PU

(Liu et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^en.PU, vtd^GL00522 has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4^en.PU/egr^KK103432

(Liu et al., 2015)

Scer\GAL4^en.PU, vtd^GL00522 has hemocyte | third instar larval stage phenotype, non-suppressible by Scer\GAL4^en.PU/egr^KK103432

(Liu et al., 2015)

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, Sse^{RNAi.147.UASp}, vtd^GL00522 has polar body nucleus phenotype

(Guo et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has ovary phenotype

(Gyuricza et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has female germline cell phenotype

(Gyuricza et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of vtd^GL00522 does not enhance precocious homologue segregation seen in oocytes with expression of SMC3^GL00518 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Co-expression of vtd^GL00522 leads to polar bodies containing 8 X-cent and 8 X-arm FISH signals in embryos with expression of Sse^{dsRNA.147.Scer\UAS.P\T} driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (polar body chromosomes replicate but fail to release arm cohesion).

(Guo et al., 2016)

Expression of vtd^GL00522 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the ord⁵/ord¹⁰ mutant background results in rudimentary ovaries lacking germ cells.

(Gyuricza et al., 2016)

The dramatic increase in apoptosis (visualized by [*]Casp3 or acridine orange staining) observed in larval wing discs upon expression of vtd^GL00522 driven by Scer\GAL4^en-e16E is increased further by co-expression of Atg1^GD7149 or Atg18a^GD12342 or park^KK107919 and can be suppressed (along with the elevated oxidative stress - monitored by levels of ROS) by co-expression of Tor^GL00156 or park^{Scer\UAS.T:Zzzz\FLAG,T:Hsap\Mito-COX4I1}. The increased frequency of aneuploid metaphases characteristic for vtd^GL00522-expressing wing discs is also significantly ameliorated by expression of Tor^GL00156.

(Liu et al., 2016)

The expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in apoptosis in the posterior compartment of the third instar larval wing disc, which is suppressed by co-expressing either Tl^GL00474, dl^JF02825, Cat^Scer\UAS.cAa, Dsp1^JF02732, egr^KK103432 or bsk^GD10555.

Co-expressing vtd^GL00522 and dl^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Bx-MS1096 (together with Dicer-2, for efficient vtd RNAi) leads to a significant increase in apoptosis in the third instar larval wing pouch.

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) results in increased numbers of hemocytes on the surface of third instar larval wing discs, as compared to controls, which are suppressed by co-expressing either Tl^GL00474, dl^JF02825, Cat^Scer\UAS.cAa, bsk^GD10555 or Rel^HM05154, but not by co-expressing egr^KK103432.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

The increased frequency of aneuploid metaphases observed in third instar larval wing discs expressing vtd^GL00522 under the control of Scer\GAL4^en-e16E is increased further by co-expression of BacA\p35^Scer\UAS.cHa.

(Liu et al., 2016)

The expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) leads to a significant increase in aneuploidy in the posterior compartment of the third instar larval wing disc, which is enhanced by co-expressing BacA\p35^Scer\UAS.cHa.

(Liu et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36786

y¹ sc^* v¹ sev²¹; P{TRiP.GL00522}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rad21^GL522

(Guo et al., 2016)

Rad21^RNAiGL522

(Guo et al., 2016)

vtd^GL00522

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close